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EGFR (epidermal growth factor receptor) gene mutation multiple detection primer probe and kit thereof

A multiplex detection, primer-probe technology, applied in the biological field, can solve problems such as inability to detect, and achieve the effect of accurate results

Pending Publication Date: 2022-04-05
普瑞斯新(上海)生物医疗科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The percentage of tumor-derived DNA fraction in total cfDNA is individually variable and often too low to detect
Finally, previous

Method used

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  • EGFR (epidermal growth factor receptor) gene mutation multiple detection primer probe and kit thereof
  • EGFR (epidermal growth factor receptor) gene mutation multiple detection primer probe and kit thereof
  • EGFR (epidermal growth factor receptor) gene mutation multiple detection primer probe and kit thereof

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Example Embodiment

[0030] Example

[0031] The EGFR gene mutation multi-retrimentary test kit of the present embodiment includes an upper primer F-T790M, downstream primer R-T790M, downstream primer R-T790M, a wild-type probe WP-T790, and a mutant probe MP-T790M, and a mutant probe. The upstream primer F-L858R, downstream primer R-L858R, wild-type probe WP-L858 and mutant probe MP-L858R, wild-type probe WP-L858 and mutant probe MP-L858R were detected, and the upper reaches of exon fraction of EGFR gene 19 Primer F-E19DEL, downstream primer R-E19DEL, wild-type probe WP-E19 and mutant probe MP-E19Del, plasmid with EGFR gene T790M mutation, plasmid with EGFR gene L858R mutation, with EGFR gene 19 Old exon fragment deleted E19DEL mutation plasmid.

[0032] The design of the primers, probes, and plasmids involved in this embodiment have the technical staff of Prissovic (Shanghai) Biomedical Technology Co., Ltd., the synthetic unit is the completion of Nanjing Jinsi Biotechnology Co., Ltd. The nucleotide ...

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Abstract

The invention relates to a multiplex detection primer probe for EGFR (epidermal growth factor receptor) gene mutation and a kit thereof. The multiplex detection primer probe comprises an upstream primer F-T790M, a downstream primer R-T790M, a wild type probe WP-T790 and a mutant type probe MP-T790M which are used for detecting the mutation of an exon T790M No. 20 of an EGFR gene, and an upstream primer F-L858R, a downstream primer R-L858R, a wild type probe WP-L858 and a mutant type probe MP-L858R which are used for detecting the mutation of an exon L858R No. 21 of the EGFR gene, the upstream primer F-E19del, the downstream primer R-E19del, the wild type probe WP-E19 and the mutant type probe MP-E19del are used for detecting deletion mutation of a No.19 exon fragment of an EGFR (epidermal growth factor receptor) gene. The EGFR gene mutation multiple detection primer probe and the kit thereof have the advantages of rapidness, high efficiency, simple preparation of a standard substance, high specificity and sensitivity of a detection system, high utilization rate of free DNA, and high accuracy.

Description

technical field [0001] The invention relates to an EGFR gene mutation detection product, which belongs to the field of biotechnology. Background technique [0002] Mutations in the EGFR gene are common in several cancer types, and these mutations often result in altered expression and activity. This type of activating mutation often occurs in epithelial cancers, especially lung cancer and glioblastoma, and is also the main driver gene of lung adenocarcinoma in my country. The morbidity and mortality of lung cancer rank first among all malignant tumors. Non-small cell lung cancer (NSCLC) is one of the most common cancers in the world and is often associated with mutations in EGFR. Upregulation and overexpression of EGFR can lead to uncontrolled cell division, leading to malignant growth of tissues and leading to cancer. A common example is a point mutation (c.2573T>G) in exon 21 of EGFR, commonly referred to as the L858R mutation, and another high-frequency mutation is t...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/686C12N15/11
Inventor 洪专潘文健
Owner 普瑞斯新(上海)生物医疗科技有限公司
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