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Separation method of disodium 5 '-inosinate

A disodium inosinate and separation method technology, applied in chemical instruments and methods, sugar derivatives, organic chemistry, etc., to achieve high purity, ensure economical efficiency, and improve the effect of crystal color

Pending Publication Date: 2022-04-08
CJ CHEILJEDANG CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Regarding the method of cultivating microorganisms and producing disodium 5'-inosinate from the culture thereof, an organic solvent can be used in the crystallization step, but a large amount of the organic solvent should be used, thereby requiring explosion-proof equipment and workers' equipment

Method used

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  • Separation method of disodium 5 '-inosinate

Examples

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Comparison scheme
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Embodiment

[0034] Example: IMP Fermentation and Isolation of IMP from Culture

[0035] 1. IMP production and microbial cultivation

[0036] Corynebacterium glutamicum was inoculated into a 30L fermentation incubator containing 18L of medium, and cultured at 31°C for 140 hours to produce 5'-inosinic acid (IMP) in the culture. The 5'-inosinic acid is secreted extracellularly and exists in the culture medium. The initial pH value of the medium is 7.2, based on 1L of distilled water, including 46g glucose, 30g fructose, 10g yeast extract, 18g KH 2 PO 4 , 42g KH 2 PO 4 , 6g urea, 10g MgSO 4 ·7H 2 O, 30 μg biotin and 5 mg thiamine hydrochloride. The pH of the final resulting culture was 6.8.

[0037] After adding NaOH at a content of 50 w / w% to the resulting culture to adjust the pH to 10.5, it was filtered through a microfilter to remove bacterial cells and obtain a supernatant. The concentration of 5'-IMP in the supernatant was measured using HPLC. The supernatant contained 140 g / L...

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Abstract

The invention provides a method for separating 5 '-inosinic acid from a microbial culture solution containing 5'-inosinic acid.

Description

technical field [0001] The invention relates to a method for isolating disodium 5'-inosinate from microbial culture. Background technique [0002] Purine derivative nucleotides such as inosinate disodium (disodium 5'-inosinate, IMP2Na) are used in the pharmaceutical sector as raw materials for the prevention and treatment of metabolic disorders, growth promoters, anticancer agents, etc., and are used in food Important substances used as food additives such as condiments in the field. Disodium inosinate is prepared by fish meat extraction method of inosinic acid (IMP), RNase decomposition method, microbial fermentation method, combination of fermentation method and synthesis method, combination of RNA chemical decomposition and synthesis, etc., said Inosinic acid is a substance naturally present in meat, fish, etc. [0003] Inosinic acid is a precursor substance used to prepare disodium inosinate, and a lot of research has been done on its preparation method. In order to ob...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/06C07H19/20
CPCC07H1/08C07H19/20C12P19/40C12N1/20C12P19/32
Inventor 崔祯化金旻锺吴昌烨林和姸金俊宇兪裁宪姜锡贤金庾賮金日出
Owner CJ CHEILJEDANG CORP