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Method for determining contents of jujuboside A and jujuboside B in Tianwang heart-tonifying preparation

A technology of jujube seed saponin and Tianwang Buxin, which is applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of low content and failure to detect jujube seed saponin B, and achieve high recovery rate and perfect quality inspection standards , measure the exact effect

Pending Publication Date: 2022-05-13
广西壮族自治区食品药品检验所
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] There have been many reports on the content determination of jujuboside in Chinese herbal decoction pieces, but there are few reports on the analysis of the content of jujuboside A and saponin B in Tianwangbuxin preparations. Relevant literature such as: He Xinrong et al. The content of jujuboside A in Tianwangbuxin pills was determined by liquid chromatography combined with evaporative light scattering detector (HPLC⁃ELSD). , 0.2750 mg / g, the linear range of jujuboside A is 22.5~360ug / mL (R 2 =0.9996), and the average recovery rate was 99.24% (RSD=1.37%). At the same time, jujuboside B in the sample was also detected, but this method may be used due to its low content and limited sensitivity of evaporative light scattering detection. Unable to detect jujuboside B content

Method used

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  • Method for determining contents of jujuboside A and jujuboside B in Tianwang heart-tonifying preparation
  • Method for determining contents of jujuboside A and jujuboside B in Tianwang heart-tonifying preparation
  • Method for determining contents of jujuboside A and jujuboside B in Tianwang heart-tonifying preparation

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Experimental program
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Effect test

Embodiment 1

[0024] 1 Instruments and reagents

[0025] Instruments: Agilent 1290 ultra-high performance liquid phase system; Agilent 6490 triple quadrupole MS system.

[0026] ML204 electronic analytical balance (Mettler-Toledo Instruments Shanghai Co., Ltd.). Jujube seed saponin A reference substance (provided by the Chinese Academy of Sciences, for content testing, batch number: 110734, content: 96.0%) jujube seed saponin B (supplied by the Chinese Academy of Sciences, for content testing, batch number: 110814-201909, content: 96.2%) ); acetonitrile was chromatographically pure, and other reagents were analytically pure. The 159 batches of Tianwang Buxin Pills are samples for the national evaluation random inspection in 2021.

[0027] 2 Investigation and methodological verification of experimental conditions of the present invention

[0028] 2.1 Selection of mobile phase: with Waters CORTECS The C18 column (2.7 µm, 4.6 mm × 150 mm) is a chromatographic column, the column temperatur...

Embodiment 2

[0082] Example 2 Sample Determination

[0083] A method for determining the content of jujube seed saponin A and saponin B in Tianwangbuxin preparations, the Tianwangbuxin preparations include Tianwangbuxin pills and Tianwangbuxin pills (concentrated pills), which are carried out by high performance liquid chromatography-mass spectrometry Determination, this assay method comprises the following steps:

[0084] (1) Chromatographic conditions: use octadecylsilane bonded silica gel as filler (4.6 mm×150mm); use 0.1% formic acid solution as mobile phase A, and use acetonitrile as mobile phase B, according to the regulations in Table 1 gradient elution;

[0085]

[0086] (2) Mass spectrometry conditions and system suitability test: Electrospray ionization source (ESI), negative ion mode, multiple reaction monitoring (MRM); drying gas temperature: 200°C; drying gas flow rate: 12L / min; atomizing gas pressure: 20 (psi); sheath gas temperature: 350°C; sheath gas flow rate: 11L / min...

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Abstract

The invention discloses a method for determining the contents of jujuboside A and jujuboside B in Tianwang heart-tonifying preparation, and belongs to the technical field of traditional Chinese medicine detection. According to the method, the contents of jujuboside A and jujuboside B in the Tianwang heart tonifying preparation are simultaneously determined by adopting a high performance liquid chromatography-mass spectrometry method. The detection method is simple to operate, accurate in determination result, high in detection efficiency, high in sensitivity and strong in specificity, and can be used for conveniently and quickly detecting the contents of jujuboside A and jujuboside B in the Tianwang heart-tonifying preparation, so that the quality of the Tianwang heart-tonifying preparation is effectively controlled, the clinical efficacy is ensured, and the clinical application prospect is broad. The quality of the Tianwang heart-tonifying preparation can be further accurately and comprehensively controlled.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine detection, and in particular relates to a method for determining the contents of jujube seed saponin A and saponin B in Tianwangbuxin preparation. Background technique [0002] Tianwang Heart-Burn preparation has the effect of nourishing yin and nourishing blood, nourishing the heart and calming the nerves; it is clinically used for heart-yin deficiency, palpitation, forgetfulness, insomnia, dreaminess, and dry stool. Tianwang Buxin preparations include Tianwang Buxin Pills and Tianwang Buxin Pills (concentrated pills), both of which are formulated with Danshen, Angelica, Shichangpu, Codonopsis, Poria, Schisandra, Ophiopogon japonicus, Asparagus, Dihuang, Scrophulariae, Composed of Polygala (made), Suanzaoren (stir-fried), Baiziren, Campanulaceae, Licorice, Cinnabar and other 16 herbs. Tianwang Buxin pills are brown-black water honey pills, brown-black small honey pills or bi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/72G01N30/86
CPCG01N30/02G01N30/34G01N30/06G01N30/7266G01N30/8606G01N2030/042
Inventor 李丽莉何颂华钟妍朱韬文丽庾延和唐萍
Owner 广西壮族自治区食品药品检验所
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