Mineralized collagen gel loaded with gingival mesenchymal stem cell exosome and preparation method thereof

Abstract

The invention discloses mineralized collagen gel loaded with gingival mesenchymal stem cell exosomes and a preparation method of the mineralized collagen gel. The gel is prepared from the gingival mesenchymal stem cell exosome, mineralized collagen and chitosan/beta-sodium glycerophosphate. According to the invention, the exosome has anti-inflammatory and osteogenesis promoting effects, the mineralized collagen has osteoinductivity, the chitosan is antibacterial and has temperature-sensitive characteristic after the beta-sodium glycerophosphate is introduced, and the gel synthesized from the components can be injected into a bone defect area in a periodontitis state so as to promote local bone repair or bone amplification. The gel can improve the treatment effect of the exosome, significantly improve the drug absorption rate of the body and the local residence rate of the drug, has good biocompatibility and biological activity, provides a novel administration approach for macromolecular drugs, is an injectable use mode, and has good application prospects.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to an exosome-loaded mineralized collagen gel for periodontal bone tissue repair and a preparation method thereof. Background technique [0002] Periodontitis is a disease characterized by the destruction of periodontal supporting tissue, and alveolar bone resorption is one of its main clinical manifestations. Periodontitis has a high incidence in adults and is the leading cause of tooth loss. Flap bone grafting is one of the effective methods for the treatment of alveolar bone defects. However, conventional bone graft materials do not contain bioactive components that promote bone tissue regeneration and lack osteoinductivity. Therefore, it is difficult to obtain bone repair using only bone graft materials. Ideal repair effect, while bone remodeling is a complex mechanism involving the interaction of osteoblasts, osteoclasts, stem cells, macrophages and other cells. [0003] Gingival...

AI Technical Summary

Problems solved by technology

Flap bone grafting is one of the effective methods for the treatment of alveolar bone defects, but conventional bone graft materials do not contain bioactive components that promote bone tissue regeneration and lack osteoinductivity, so it is difficult to achieve bone repair with bone graft materials alone. Ideal repair effect, while bone remodeling is a complex mechanism involving the interaction of various cells such as osteoblasts, osteoclasts, stem cells, macrophages, etc.

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