Kit for rapidly detecting hybrid pilose antler based on RAA amplification

Abstract

The invention relates to primers for detecting hybrid cornu cervi pantotrichum based on an RAA method and a kit containing the primers. The primers comprise an upstream primer 014F2, a red deer specific downstream primer 014R2 and a sika deer specific downstream primer 014S2, the 5 '-end of the upstream primer is labeled with biotin, and the 5'-end of the downstream primer is labeled with digoxin; the kit comprises the primers, a reaction reagent and a colloidal gold test strip. The allele-specific amplification primer is designed based on the SNP site, the colloidal gold test strip SNP typing detection method based on allele-specific recombinase-mediated isothermal amplification is established, and a simple and effective scientific means is provided for identification of the flower-horse hybrid deer.

Description

technical field [0001] The invention belongs to the field of molecular biology, and specifically relates to a method for identifying hybrid antlers based on RAA amplification. Background technique [0002] China is the third largest deer-raising country in the world, mainly producing velvet. Generally speaking, deer antlers are stably harvested from 3-year-old sika deer and red deer, once a year in July, twice in April and September, and the weight of Ergang antlers does not exceed 120g, and the weight of Sancha antlers does not exceed 400g. Long-term low yields have limited the production of deer antlers. In recent years, with the integration of the world economy and the globalization of the deer industry market, deer farming countries that mainly use meat have introduced a large number of deer antlers and their by-products into the Chinese market, which has brought enormous pressure to the Chinese deer industry. In order to improve the international competitive advantage...

AI Technical Summary

Problems solved by technology

Although hybridization technology has promoted the development of the antler industry, it has also added difficulties to the supervision of the antler market

In addition, since hybrid velvet antler is not included in the scope of medicinal velvet velvet stipulated in the "Chinese Pharmacopoeia", its me

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