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Method for evaluating antioxidant effects of cosmetic products and raw materials

An anti-oxidation and cosmetic technology, applied in analytical materials, instruments, measuring devices, etc., can solve the problems of inability to achieve close, intuitive, and rapid evaluation of anti-oxidation effects, large differences in human skin physiological functions, and unstable detection indicators. Objective antioxidant efficacy, small individual differences, and small workload

Pending Publication Date: 2022-06-28
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Such as DPPH scavenging ability evaluation method, SOD-like activity evaluation method, hydrogen peroxide scavenging ability evaluation method, and lipid peroxide production inhibition evaluation method, but according to biochemical experiments, it is impossible for antioxidant / antioxidant-containing cosmetic substrates to be used on the human body Nearly, intuitively and quickly evaluate the antioxidant effect after use
The existing detection technology for the antioxidant efficacy of cosmetics and raw materials has the problems of unstable detection indicators, large individual differences in the detection of cosmetic raw materials and cosmetic product carriers, large sampling workload, and large differences with the physiological functions of human skin.

Method used

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  • Method for evaluating antioxidant effects of cosmetic products and raw materials
  • Method for evaluating antioxidant effects of cosmetic products and raw materials
  • Method for evaluating antioxidant effects of cosmetic products and raw materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The method for evaluating the antioxidant efficacy of the product in this example is as follows: using light to induce the oxidation of the carrier (using the 3D skin model as the carrier), and using the content of oxidized protein in the carrier and the thickness of the stratum corneum as indicators to evaluate the antioxidant efficacy of the product.

[0048] Experimental reagents: 2-(N-morpholine)ethanesulfonic acid (MES) (Beijing Soleibao Technology Co., Ltd.); Fluorescein-5-Thiosemicarbazide (FTSC) (Thermo fisher); Vitamins C magnesium phosphate (APM) (Shanghai McLean Biochemical Technology Co., Ltd.); xylene (Bai Lingwei Technology Co., Ltd.); paraformaldehyde, VanGieson (VG) staining solution, hematoxylin staining solution, eosin staining solution, D- PBS (Beijing Soleibo Technology Co., Ltd.); SkinEthic TM RHE skin model, SkinEthic TM In vitro artificial skin model maintenance solution (Shanghai Sianfunuo Biotechnology Co., Ltd.).

[0049] Experimental equipm...

Embodiment 2

[0071] The method for evaluating the antioxidative effect of the product in this embodiment is as follows: detecting the antioxidative effect of the product through the effect of the product on the human stratum corneum.

[0072] Experimental reagents: D-PBS, 2-(N-morpholine)ethanesulfonic acid (MES) (Beijing Soleibo Technology Co., Ltd.); Vitamin C Magnesium Phosphate (APM) (Shanghai McLean Biochemical Technology Co., Ltd.); Fluorescein - 5-Thiosemicarbazide, FTSC (Thermo fisher); Xylene (Aladdin Reagent Co., Ltd.); Dimethyl sulfoxide (DMSO), Sigma Company.

[0073] Experimental equipment: ME204E electronic analytical balance, pH meter (METTLER TOLEDO); fluorescence inverted microscope (Zeiss), UV light meter (Luchang, Taiwan).

[0074] Reagent preparation: prepare 0.1M MES-Na buffer (pH 5.5), FTSC solution (dissolve FTSC in DMSO, prepare 20mM, and store at -20°C for later use. Dilute 1000 times with 0.1M MES-Na buffer before use , the final concentration is 20 μM).

[0075...

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Abstract

The invention belongs to the technical field of cosmetics, and discloses a method for evaluating the antioxidant efficacy of cosmetic products and raw materials. The method comprises the following steps: (1) respectively mixing a product, a solvent and a skin model, and incubating to obtain a product group and a blank group; (2) respectively carrying out light irradiation on the product group and the blank group to obtain a product light irradiation group and a blank light irradiation group; and (3) respectively incubating the skin models in the product light irradiation group and the blank light irradiation group, and then detecting indexes of the skin models in the product light irradiation group and the blank light irradiation group, the indexes being at least one of oxidized protein content, cuticle thickness and tissue morphology. According to the method, at least one of the oxidized protein content, the cuticle thickness and the tissue form is taken as an index, the anti-oxidation and skin barrier protection effects of the product are evaluated, and the result is more objective; compared with an anti-oxidation free radical detection method, the method has the advantage that the anti-oxidation effect of the product on the skin can be directly reflected.

Description

technical field [0001] The invention belongs to the technical field of cosmetics, and in particular relates to a method for evaluating the antioxidant efficacy of cosmetic products and raw materials. Background technique [0002] Skin photoaging is a process of slow development over time. Different light wavelengths, irradiation doses, physiological factors, pathological factors, etc. can affect the occurrence of skin photoaging. [0003] In order to resist the oxidative damage to the skin caused by the environment such as light, cosmetics companies add antioxidant substances to cosmetics, and at the same time hope that some method can be used to understand the antioxidant capacity of antioxidant agents or cosmetics when they are directly applied to the skin. The methods for detecting antioxidant capacity generally include pure chemical detection methods and biological tissue detection methods. Such as DPPH scavenging ability evaluation method, SOD-like activity evaluation ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N17/00G01N33/00
CPCG01N17/004G01N33/00
Inventor 汤丹戴结玲刘贤钊陈炎欢孙红梅
Owner INFINITUS (CHINA) CO LTD
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