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Double-palindromic DNAzyme nanowire sensor as well as preparation method and application thereof

A nanowire and sensor technology, applied in the field of double palindromic DNAzyme nanowire sensor and its preparation, can solve the problems of high cost and operational complexity, insufficiently satisfying target signal transduction, limited background suppression, etc. Simple operation method and low cost effect

Pending Publication Date: 2022-07-08
江苏艾唯德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But in some cases, rational arrangement of F / Q pairs alone to reduce background of DNAzyme is not enough for target signaling, because background suppression is limited in these cases
Often needs to be combined with other signal transduction strategies (e.g., isothermal amplification of nucleic acids using enzymes) to improve their detection performance, but this brings high cost and operational complexity

Method used

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  • Double-palindromic DNAzyme nanowire sensor as well as preparation method and application thereof
  • Double-palindromic DNAzyme nanowire sensor as well as preparation method and application thereof
  • Double-palindromic DNAzyme nanowire sensor as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Detect the copper ions in the spiked tap water (the copper ion concentration is 50 nmol / L), and the specific steps are as follows:

[0042]S1. Preparation of double palindromic DNAzyme nanowire sensor:

[0043] Add 2μL, 10μM substrate chain, 2μL, 10μM double palindrome chain and 6μL HEPES buffer to the PCR tube, mix well, heat and denature at 95°C for 5min, and then reduce the temperature by 1°C for 1min to cool down to 25°C, maintained at a constant temperature of 25°C for 30 minutes, so that the formed double palindromic DNAzyme nanowire structure is more stable, and then stored at 4°C for later use.

[0044] S2. Feasible steps for double palindromic DNAzyme nanowire sensor to detect copper ions:

[0045] The 20 μL copper ion detection system includes 10 μL double palindromic DNAzyme nanowire structure mixture, 2.5 μL tap water composite sample containing spiked copper ions, and 7.5 μL HEPES buffer, mixed well and incubated at 37°C for 40 min.

[0046] S3. Detection...

Embodiment 2

[0061] Detect the copper ions in the spiked lake water (the copper ion concentration is 50nmol / L), and the specific steps are as follows:

[0062] S1. Preparation of double palindromic DNAzyme nanowire sensor:

[0063] Add 2μL, 10μM substrate chain, 2μL, 10μM double palindrome chain and 6μL HEPES buffer to the PCR tube, mix well, heat and denature at 95°C for 5min, and then reduce the temperature by 1°C for 1min to cool down to 25°C, maintained at a constant temperature of 25°C for 30 minutes, so that the formed double palindromic DNAzyme nanowire structure is more stable, and then stored at 4°C for later use.

[0064] S2. Feasible steps for double palindromic DNAzyme nanowire sensor to detect copper ions:

[0065] The 20 μL copper ion detection system includes 10 μL double palindromic DNAzyme nanowire structure mixture, 2.5 μL tap water composite sample containing spiked copper ions, and 7.5 μL HEPES buffer, mixed well and incubated at 37°C for 40 min.

[0066] S3. Detectio...

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Abstract

The invention discloses a double-palindromic DNAzyme nanowire sensor as well as a preparation method and application thereof, and the double-palindromic DNAzyme nanowire sensor is formed by assembling a plurality of double-palindromic DNAzyme nanowire monomers under the action of a palindromic sequence through the inverted readability of the palindromic sequence; the double-palindromic DNAzyme nanowire monomer comprises a double-palindromic enzyme chain and a substrate chain; the fluorophore is labeled on a substrate chain, and the quenching group is labeled on a double-palinase chain; the 5'end and the 3 'end of the double-palindromic enzyme chain are respectively connected with a section of palindromic sequence; according to the invention, double palindromic chain reaction is introduced into simple engineered DNA nano assembly, and a palindromic sequence is grafted to a common oligosaccharide nucleotide probe, so that intermolecular nucleic acid hybridization can be easily realized by only one probe; meanwhile, the double-palindromic DNAzyme nanowire sensor can sensitively detect heavy metal ions in one step through a palindromic-induced fluorescence co-quenching effect and specific cutting of a substrate chain.

Description

technical field [0001] The present invention relates to the technical field of a DNAzyme nanometer sensor, and more particularly to a double palindromic DNAzyme nanowire sensor and a preparation method and application thereof. Background technique [0002] The natural background content of heavy metals in the water body does not have obvious harm, but with the expansion of the city and the acceleration of the industrialization process, a large amount of industrial wastewater and domestic sewage are discharged into the water body. The food chain and other means have a series of toxic effects on the organisms in the water body, and finally endanger human health. Heavy metals are very dangerous pollutants, which are often accumulated in organisms for a long time and cannot be degraded, and even in extremely small amounts can have adverse consequences. Various ecosystems are affected by heavy metals to varying degrees. Heavy metals are deposited into the human body through the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6421G01N2021/6432G01N2021/6439
Inventor 徐建国尚慧杰姚丽黄纯尹余波
Owner 江苏艾唯德生物科技有限公司
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