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Method for rejuvenating trichoderma viride degenerated strain through trace element compound amino acid

A compound amino acid, Trichoderma viride technology, applied in microorganism-based methods, biochemical equipment and methods, fungi and other directions, can solve the problems of difficult popularization and application, time-consuming and labor-intensive, low success rate, etc., and achieves remarkable rejuvenation effect. Time consuming and low success rate

Pending Publication Date: 2022-07-12
JILIN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The rejuvenation method in the host needs to find a suitable host for co-cultivation and then isolate and purify the target strain, which is time-consuming and labor-intensive
The pure species isolation method selects the single cells that still retain the typical traits in the degenerate bacterial population and isolates them, and then expands the culture. For filamentous fungi that do not grow spores, it is necessary to use a sterile knife to cut the top part of the hyphae for isolation and transplantation. This method Multiple transfer cultures of degenerated strains are required, which takes a long time and has a low success rate
Antibiotic law, although feasible, but the current national control of antibiotics is very strict, it is not easy to promote and apply

Method used

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  • Method for rejuvenating trichoderma viride degenerated strain through trace element compound amino acid
  • Method for rejuvenating trichoderma viride degenerated strain through trace element compound amino acid
  • Method for rejuvenating trichoderma viride degenerated strain through trace element compound amino acid

Examples

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Embodiment 1

[0035] Example 1 Isolation and identification of endophytic fungi of ginseng

[0036] 1. Bacterial isolation and purification

[0037] The ginseng used for separation was collected from four-year-old Datian ginseng in Houjiadian Village, Longquan Town, Jingyu County, Baishan City, Jilin Province.

[0038]The specific operation of separation is as follows: take the root and stem samples of ginseng plant, wash the surface soil with tap water, rinse with sterile water 3-4 times, and then soak in 75% ethanol for 1 min in the ultra-clean workbench for the first disinfection. After rinsing with sterile water for 3-4 times, use 25% sodium hypochlorite solution to immerse for 20 minutes for the second disinfection. After disinfection, rinse the ginseng with sterile water for 3-4 times, and dry it with sterile filter paper. Smear the sterile water of the last rinse sample on the PDA plate, repeat 3 times; incubate at 25°C for 5 days, observe the growth of no colonies, if no colonies a...

Embodiment 2

[0051] Embodiment 2 A kind of method of trace element compound amino acid rejuvenation Trichoderma viride degenerated strain

[0052] Example 1 Trichoderma viride strains are degraded when subcultured or unsuitable culture conditions (factors such as medium components, temperature, etc.) isolated and extracted, such as reduction or loss of spore production, and obvious reduction of spore pigments. The present embodiment provides a kind of rejuvenation method for the Trichoderma viride that degenerates after subculture 15 times for PDA medium (potato glucose water medium finished product 26g / L, agar 15g / L, distilled water 1L), and the specific content is as follows:

[0053] A method for rejuvenating the degenerated strain of Trichoderma viride with trace element compound amino acids, the method is: the Trichoderma viride ZXF-LSMM-1 strain (see figure 2 ) was transferred to the rejuvenation medium, and cultivated at 28°C for 7 days; then, the relatively robust Trichoderma viri...

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Abstract

The invention relates to a method for rejuvenating a degenerated trichoderma viride strain by trace element compound amino acid, which comprises the following steps: inoculating hyphae of the degenerated trichoderma viride strain on a rejuvenation culture medium, culturing at 28 DEG C for 7-10 days, and transferring for 1-2 times, wherein the rejuvenation culture medium comprises an improved PDA (potato dextrose agar) culture medium and 3-7mL / L of 5mmol / L lysine; the improved PDA culture medium is prepared from 26 g / L of a potato glucose water culture medium finished product (PDW), 15 g / L of agar, 0.3-1 g / L of MgSO4. 7H2O, 5-10 mg / L of CuSO4. 5H2O, 5-10 mg / L of FeSO4. 7H2O and 1 L of distilled water. According to the method, a traditional strain rejuvenation method is broken through, simple, economical and harmless amino acid and a culture medium with a proper nutrient element ratio are adopted, and the robust green trichoderma viride strain which is large in sporulation quantity and dark green in spore color is obtained.

Description

technical field [0001] The invention belongs to the technical field of fungal rejuvenation, in particular to a method for rejuvenating a degenerate strain of Trichoderma viride with a trace element compound amino acid. Background technique [0002] Trichoderma viride belongs to Hyphomycetes, Moniliales, Moniliales, Trichoderma, and is an important biocontrol fungus in the biological field. It plays a positive role in promoting plant growth, preventing plant diseases and insect pests, and degrading pesticide residues. Trichoderma viride is an environment-friendly fungus that is widely used in the production of microbial fertilizers. Since chemical fertilizers and pesticides cause great harm to human health and the environment, microbial fertilizers dominated by Trichoderma viride are considered to be chemical fertilizers. replacement of. [0003] As a multifunctional biocontrol fungus, Trichoderma viride has attracted much attention. However, in the research, it was found ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/885
CPCC12N1/14
Inventor 周晓馥宋明霞徐洪伟
Owner JILIN NORMAL UNIV
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