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Application of mitomycin C in preparation of antidepressant drug

A mitomycin and anti-depressant technology, applied in the field of biomedicine, can solve the problems of easy relapse and lack of mitomycin C, etc., and achieve the effect of alleviating inflammation and inhibiting the increase of inflammation level

Active Publication Date: 2022-07-15
INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it can relieve depressive symptoms to a certain extent, there are limitations such as easy relapse after stopping treatment
There is no report of mitomycin C in antidepressant

Method used

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  • Application of mitomycin C in preparation of antidepressant drug
  • Application of mitomycin C in preparation of antidepressant drug
  • Application of mitomycin C in preparation of antidepressant drug

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0050] (1) Experimental method

[0051] The corticosterone-induced PC12 cell injury model used is recognized as an ideal in vitro model of depression, which can simulate the pathological state of depression and is suitable for investigating the antidepressant effect of mitomycin C.

[0052] The present invention adopts CCK8 method to evaluate the protective activity of mitomycin C on PC12 cells damaged by corticosterone. PC12 cells were trypsinized and prepared in complete medium to contain 1 × 10 cells per ml 5 The suspension was inoculated into a 96-well culture plate, 100 μL per well, and placed at 37°C, 5% CO 2 Incubator culture. After 24 hours, the cell liquid was discarded, the blank group was added with complete medium, and the treatment group was added with complete medium with concentrations of 0.1, 0.5, 2.5, 5, 10, 20, 40, 80, and 160 μM mitomycin C, respectively. Six duplicate wells were set for each concentration; 10 μL of CCK8 solution was added to each well af...

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Abstract

The invention relates to the technical field of biological medicines, in particular to application of mitomycin C in preparation of antidepressant drugs. The invention provides application of mitomycin C in preparation of antidepressant drugs. The mitomycin C can effectively inhibit the increase of PC12 cell inflammation level caused by corticosterone induction and relieve inflammatory response, thereby playing an anti-depression effect. The corticosterone-induced depression model can simulate the pathological process of depression, and can be used for efficacy evaluation and mechanism exploration of antidepressant drugs. The traditional Chinese medicine composition can provide scientific basis for clinical medication of depression.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of mitomycin C in the preparation of antidepressant drugs. Background technique [0002] Depression is the fourth largest disease in the world. The medical prevention and treatment of depression in my country is still in a situation of low recognition rate. The recognition rate of hospitals above prefecture-level cities is less than 20%, and less than 10% of patients have received related treatment. Drug treatment; in addition, the incidence of depression (and suicide) has begun to show a trend of younger age (college, and even primary and secondary school students). [0003] Current first-line antidepressants include fluoxetine, paroxetine, sertraline, fluvoxamine, and citalopram, targeting the serotonin and norepinephrine pathways. Although depressive symptoms can be alleviated to a certain extent, there are limitations such as easy relapse after stopping tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/407A61P25/24
CPCA61K31/407A61P25/24
Inventor 郭秋岩王继刚徐承超王启新张迎王晨马昂徐丽婷吕海宁
Owner INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI