Virus exposure profile for detecting early hepatocellular carcinoma

A hepatocellular carcinoma, virus technology, applied in the field of identifying subjects with early-stage hepatocellular carcinoma, can solve problems such as non-existence

Pending Publication Date: 2022-07-15
US DEPT OF HEALTH & HUMAN SERVICES
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

VES is based on the presence or absence of antibodies against a particular virus strain in the subject

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Virus exposure profile for detecting early hepatocellular carcinoma
  • Virus exposure profile for detecting early hepatocellular carcinoma
  • Virus exposure profile for detecting early hepatocellular carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Example 1: Method

[0078] This example describes the materials and experimental procedures used for the studies described in Example 2.

[0079] Participants and VirScan Analysis

[0080] The patient cohort consisted of 899 sequentially enrolled participants (clinicaltrials.gov number: NCT0091375), including 150 HCC cases, 337 CLD as at-risk individuals (HR or AR, used interchangeably), and 412 healthy volunteers as an age- and sex-matched population control (PC) ( Figure 1A ).

[0081] study cohort

[0082] UMD queue. To measure virus-host interactions, 899 participants were recruited. Participants were grouped into (1) a population control group (PC, n=412) if they were relatively healthy without any liver disease diagnosis; (2) if diagnosed with chronic liver disease (hepatitis B virus (HBV), hepatitis C virus (HCV), hepatitis D virus (HDV), aflatoxins from fungal contamination, alcohol, nonalcoholic fatty liver disease (NAFLD), and nonalcoholic steatohepatit...

Embodiment 2

[0107] Example 2: Viral Exposure Signature (VES) for Diagnosis of Hepatocellular Carcinoma (HCC)

[0108] This example describes the development of two viral exposure signatures - the first VES based on detection of 61 virus strains and the second VES based on detection of 31 virus strains - to identify subjects at risk for HCC.

[0109] OVERVIEW OF VIRUS EXPOSURE CHARACTERISTICS

[0110] VirScan applied a phage display library covering 93,904 viral epitopes, representing 206 human virus species and over 1000 virus strains, to screen for prior exposure history (Xu et al., Science 2015;348:aaa0698). Phage particles with epitopes recognized by participant antibodies were immunoprecipitated (Phage-IP), followed by sequencing of the encoded DNA barcodes ( Figure 1A ). A case-control design of the Maryland (NCI-UMD) cohort was used to characterize virus exposure. Enrollment and enrolment of study subjects at Figure 5E As outlined in CONSORT guidelines (Schulz et al., BMJ340:c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

Virus exposure signatures (VESs) are described that can identify early, pre-symptomatic hepatocellular carcinoma (HCC) in a risk patient. VESs are developed using serological analysis and synthetic virome techniques to identify unique viral peptide epitopes corresponding to 61 viruses. Methods of identifying subjects with early (pre-symptom) HCC using VESs are described.

Description

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS [0002] This application claims the benefit of US Provisional Application No. 62 / 914,138, filed October 11, 2019, which is incorporated herein by reference in its entirety. technical field [0003] The present disclosure relates to viral exposure signatures and their use for identifying subjects with early stage (presymptomatic) hepatocellular carcinoma. [0004] Statement of Government Support [0005] This invention was made with government support under Project No. Z01-BC010313 awarded by the National Institutes of Health. The government has certain rights in the invention. Background technique [0006] Hepatocellular carcinoma (HCC) is considered a virus-related malignancy of which hepatitis B and C viruses (HCV and HBV) are the major etiologies (Farazi et al., Nat Rev Cancer 2006; 6:674-687). Viral hepatitis causes inflammation and chronic liver disease (CLD), which can lead to fibrosis, cirrhosis, and ultimately HCC...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/6804C12N15/10C12Q1/6883
CPCC12Q1/6883C12Q1/6804C12N15/1037C40B40/02Y02A50/30A61P1/16G01N33/56983G01N33/57438
Inventor 王心伟J·刘W·唐
Owner US DEPT OF HEALTH & HUMAN SERVICES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap