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Burdock root polysaccharide prepared by utilizing rhizopus nigricans fermentation as well as production process and application of burdock root polysaccharide

A production process, the technology of Rhizopus niger, applied in the directions of fermentation, biochemical equipment and methods, and medical preparations containing active ingredients, etc., can solve the problems of limited functions and complex burdock polysaccharide components, and achieve high tumor inhibition rate, significant The effect of regulating immunity, anti-tumor, and vigorous reproduction

Active Publication Date: 2022-07-29
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this patent discloses the preparation method of burdock polysaccharides, it only uses physical methods to extract polysaccharides, and the obtained burdock polysaccharides have complex components and limited functions.

Method used

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  • Burdock root polysaccharide prepared by utilizing rhizopus nigricans fermentation as well as production process and application of burdock root polysaccharide
  • Burdock root polysaccharide prepared by utilizing rhizopus nigricans fermentation as well as production process and application of burdock root polysaccharide
  • Burdock root polysaccharide prepared by utilizing rhizopus nigricans fermentation as well as production process and application of burdock root polysaccharide

Examples

Experimental program
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Effect test

Embodiment 1

[0037] A production process utilizing Rhizopus niger fermentation to prepare burdock root polysaccharide, comprising the following steps:

[0038](1) Insert Rhizopus niger into a dish containing potato dextrose agar medium (PDA), cultivate at 28°C for 5 days until white mycelium covers the dish and grow black spores, pick 1×1 cm with an inoculating needle The hyphal block of Rhizopus niger was transferred to a culture bottle containing potato dextrose agar medium, and continued to culture for 3 days until a large number of black spores appeared, rinse the Rhizopus niger in the culture bottle with sterile water, collect the spores and count them with a counting plate , resulting in a spore density of 1 × 10 5 ~10 8 A / mL Rhizopus niger spore suspension;

[0039] (2) Select 200g of fresh burdock root washed, peeled and chopped, add it to a 3L shake flask of 1000mL potato dextrose liquid medium, and after wet sterilization, the spore density is 1 according to 2% volume ratio. ×...

Embodiment 2

[0058] A production process utilizing Rhizopus niger fermentation to prepare burdock root polysaccharide, comprising the following steps:

[0059] (1) Insert Rhizopus niger into a dish containing potato dextrose medium (PDA), cultivate at 28°C for 6 days until white mycelium covers the dish and grow black spores, pick 1×1 cm with an inoculating needle The mycelial block of Rhizopus niger was transferred to a culture flask containing potato dextrose agar medium, and continued to culture for 4 days until a large number of black spores appeared. The Rhizopus niger in the culture flask was washed with sterile water, and the spores were collected and counted with a counting plate. Get a spore density of 1×10 5 A / mL Rhizopus niger spore suspension;

[0060] (2) choose fresh burdock root, obtain burdock root water-insoluble matter after hot water extraction method, add 250g burdock root water-insoluble matter into 3L triangular flask of 1000mL potato glucose liquid medium, after wet...

Embodiment 3

[0065] A production process utilizing Rhizopus niger fermentation to prepare burdock root polysaccharide, comprising the following steps:

[0066] (1) Insert Rhizopus niger into a dish containing potato dextrose medium (PDA), cultivate at 28°C for 4 days until white mycelium covers the dish and grow black spores, pick 1 × 1 cm with an inoculating needle The mycelial block of Rhizopus niger was transferred to a culture flask containing potato dextrose agar medium, and continued to cultivate for 2 days until a large number of black spores appeared. Rinse the Rhizopus niger in the culture flask with sterile water, collect the spores and count them with a counting plate. Get a spore density of 1×10 8 A / mL Rhizopus niger spore suspension;

[0067] (2) select fresh burdock root, obtain burdock root water-insoluble matter after hot water extraction method, and pulverize the burdock root water-insoluble matter after drying at 60 ° C to obtain burdock root water-insoluble matter dry p...

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Abstract

The invention relates to burdock root polysaccharide prepared by utilizing rhizopus nigricans fermentation as well as a production process and application of the burdock root polysaccharide. The weight-average molecular weight of the burdock root polysaccharide is 5 * 10 < 4 >-1 * 10 < 6 > Da, and the molar content of galactose and galacturonic acid in the burdock root polysaccharide is greater than 50%. The invention further provides a production process for preparing the burdock root polysaccharide through rhizopus nigricans fermentation. According to the method disclosed by the invention, the burdock root polysaccharide RB3 component is obtained by fermenting the burdock root and the burdock root water extraction residues and then separating and purifying by utilizing the characteristics that the rhizopus nigricans are vigorous in reproductive capacity, rich in biological enzyme system and relatively strong in biotransformation capability; research results of in-vitro tumor cell experiments and in-vivo tumor-bearing mouse experiments show that the burdock root polysaccharide RB3 has relatively significant immune regulation and anti-tumor effects, can be applied to the fields of health foods and medical drugs, and provides scientific support for development and application of rhizopus nigricans and burdock resources.

Description

technical field [0001] The invention relates to a burdock root polysaccharide prepared by fermentation of Rhizopus niger and its production process and application, belonging to the technical field of medicine and health food. Background technique [0002] Cancer is the second most common cause of death globally. According to the World Cancer Report from the World Health Organization IARC, there will be 4.57 million new cancers in China in 2020, accounting for 23.7% of the world's new cancers and 30% of cancer deaths. Among the main cancer types in my country, the highest cancer types are lung cancer, breast cancer, gastric cancer, colorectal cancer, liver cancer, and esophageal cancer. In addition, the incidence of malignant tumors maintains an annual increase of about 3.9%, and the mortality rate maintains an annual increase of 2.5%. Compared with developed countries such as the United States, China has more limited cancer treatment options. On the one hand, traditional ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C08B37/00A61K31/713A61K31/513A61P37/02A61P35/00A23L33/125A23L33/10C12R1/845
CPCC12P19/04C08B37/0003C08B37/006A61K31/713A61K31/513A61P37/02A61P35/00A23L33/125A23L33/10A23V2002/00A61K2300/00A23V2200/308A23V2200/324
Inventor 许晅孟迎李璋马群飞鹿艳陈靠山
Owner SHANDONG UNIV
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