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Method for extracting and separating 1,3-propylene glycol from microbial fermented liquor

A microbial fermentation liquid and propylene glycol technology, applied in the field of separation 1, can solve the problems of small separation capacity, high cost, and hindering the evaporation of fractions, and achieve the effects of reducing losses, reducing costs, and saving water resources

Inactive Publication Date: 2005-02-23
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve some problems in the process of producing 1,3-propanediol follow-up separation and purification products by fermentation method, such as high cost, small separation capacity, salt crystallization and viscous substances hindering fraction evaporation in the later stage of vacuum distillation, etc. problem, and propose a kind of effective method for separating 1,3-propanediol from fermented liquid, make microbial fermentation method produce 1,3-propanediol technically, economically feasible

Method used

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Examples

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Embodiment

[0025] The fermented broth used in this example is obtained by batch feeding glycerol fermentation of Klebsiella pneumoniae to produce 1,3-propanediol, wherein the concentration of 1,3-propanediol is 63 g / l.

[0026] Klebsiella (K.pneumoniae) was purchased from the China General Microorganism Culture Collection (CGMCC), strain number: 1.1736.

[0027] The separation process is divided into five steps:

[0028] 1) Take 350ml of fermentation broth and carry out vacuum distillation in a rotary evaporator, the vacuum degree is 0.088MPa, the temperature is controlled to be ≤75°C, and concentrated to 60-80ml;

[0029] 2) After standing to cool to room temperature, add 140ml of 95% industrial ethanol, stir for 15 minutes, let stand for 5 hours, then centrifuge at 3000rpm for 10 minutes, and take the supernatant;

[0030] 3) Add 70ml of 95% industrial ethanol to the precipitate, stir for 15 minutes, centrifuge at 3000rpm for 10 minutes, and take the supernatant;

[0031] 4) Mix the ...

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Abstract

The present invention belongs to the field of biological engineering technology, in particular, it relates to a method for extracting and separating 1,3-propanediol from fermented liquor. It includes the following steps: directly distilling and concentrating fermented liquor with bacteria to 1 / 2-1 / 20 of original volume, cooling, adding ethyl alcohol or methyl alcohol, n-propyl alcohol, isopropyl alcohol, acetone or butanone according to volume ratio of 1:0.3-1:5, settling or filtering or 1000-4500 rpm centrifugal separation and precipitation, taking supernatant fluid, then using alcohol or ketone to wash and precipitating, then settling or filtering or 1000-1500 rpm centrifugal separation and precipitation, taking supernatant fluid, then recovering alcohol or ketone from said supernatant fluid by means of distillation or rectification.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to the separation technology of microbial fermentation liquid, in particular to a method for separating 1,3-propanediol from microbial fermentation liquid. Background technique [0002] As we all know, 1,3-propanediol is an important chemical raw material, which has the same purpose as ethylene glycol, 1,2-propanediol and 1,4-butanediol, but it is a new type of polyester synthesized from terephthalic acid The material polytrimethylene terephthalate (PTT) has excellent characteristics that many polyester materials do not have, such as nylon-like elastic recovery, anti-ultraviolet, ozone and nitrogen oxide colorability, low static electricity, low water adsorption, Good continuous printing and biodegradability without adding any special chemicals in the full color range. All of these show the bright application prospect of 1,3-propanediol, but its high price hinders its applicat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C29/76C07C31/20C12P7/18
Inventor 修志龙张代佳高素军陈曦
Owner DALIAN UNIV OF TECH