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Method for raising yield of fucose produced from microzyme by using high pressure

A technology for yeast and trehalose, applied in the direction of fermentation and the like, can solve the problems of low production yield and high cost of trehalose, and achieve the effects of increasing trehalose yield, reducing production cost and simple process

Inactive Publication Date: 2005-08-24
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In view of the above problems, the present invention solves the problems of low production yield and high cost of directly extracting trehalose from yeast cells

Method used

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  • Method for raising yield of fucose produced from microzyme by using high pressure

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Experimental program
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Effect test

Embodiment 1

[0019] Take 1-2 rings of slant yeast strains and transfer them to Erlenmeyer flasks filled with seed medium, and culture them at a constant temperature of 30°C for 16h. Add pre-sterilized yeast medium into the fermenter, and insert the cultivated yeast seed culture solution according to the inoculum amount of 10%. Then put them in a fermenter with a temperature of 30° C. and a ventilation rate of 1.5 vvm for expanded cultivation for 16 hours. The expanded yeast culture solution was placed in a high-pressure reactor, and then the pressure was increased to 0.1 MPa, and the constant pressure was maintained for 2 hours for treatment, the stirring speed was 120r / min, and the reaction temperature was 30°C. During autoclaving, changes in pressure must be made at a constant rate. Under this condition, the yield of trehalose was increased by about 20% compared with the normal pressure control condition.

Embodiment 2

[0021] In this embodiment, the seed cultivation temperature is 25° C., the reaction pressure in the high-pressure reaction vessel is 0.5 MPa, and the others are the same as in Embodiment 1. Under this condition, the yield of trehalose is increased by more than 20% compared with the normal pressure control condition.

Embodiment 3

[0023] In this embodiment, the expanded culture temperature is 25° C., constant temperature culture is 16 hours, and the reaction pressure in the high-pressure reaction vessel is 0.75 Mpa. Others are the same as in Embodiment 1. Under this condition, the yield of trehalose was increased by about 25% compared with the normal pressure control condition.

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Abstract

A high-pressure method for increasing the output of yeast's mycose includes yeast seed culture, amplification culture, high-pressure stimalation under 0.1-1.0 MPa for 2-4 hrs while stirring at 50-300 rpm, and reacting at 30-35 deg.C. Its advantage is high outpt of mycose.

Description

technical field [0001] The invention belongs to a technology for obtaining high-yield trehalose, in particular to a production technology for directly using high pressure to increase the yield of yeast trehalose. Background technique [0002] Trehalose (Trehalose, α-D-glucopyranosyl-α-D-glucopyranoside) is a non-reducing disaccharide composed of 2 furan ring glucose molecules combined with α-1,1 glycosidic bonds through the hemiacetal hydroxyl group. The molecular formula is C 12 h 22 o 11 2H 2 O. The molecular structural formula is: [0003] [0004] The common trehalose in nature is α, α-type, and the other two optical isomers α, β-type and β, β-type are relatively rare. Since it was obtained from the ergot fungus of rye by Wiggers in 1853, it was later found to be widely present in animals, plants and microorganisms in nature, especially fungi such as yeast and mold. Trehalose is the most stable natural disaccharide. It does not cause Fehling's reagent to reduce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/12
Inventor 贾士儒范志华孙爱友乔长晟苏建宇
Owner TIANJIN UNIV OF SCI & TECH