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Extraction of phosphatidyl serine from brains of animals
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A phosphatidylserine, animal brain technology, applied in the direction of edible phospholipid composition, food science, protein food ingredients, etc.
Inactive Publication Date: 2007-04-04
SHANDONG NORMAL UNIV
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Problems solved by technology
After many repeated experiments, this method is not suitable for extracting PS from the brains of cattle and other animals.
Method used
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Examples
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Embodiment 1
[0017] 1. Slice fresh bovine brain (or frozen bovine brain), ventilate and dry at 60°C, or dry in the sun or dry under natural conditions. The lower the drying temperature, the better, and the best low temperature drying, broken into pieces of about 0.3cm Granules, add acetone of 4 times the volume to the dried bovine brain granules, stir and extract 4 times at room temperature, each time for 2 hours, remove the acetone supernatant, and steam the residue until there is no acetone smell;
[0018] 2. Add 4 times the volume of ammonia-ethanol solution of pH=9 to the evaporated residue, stir and extract 4 times, each time for 2 hours, evaporate the residue, and the extract can be used to re-extract lecithin and cephalin;
[0019] 3. Add ether to the evaporated residue to obtain an ether extract, filter and evaporate most of the ether until the volume of the remaining ether solution is 4 times that of the dry matter extracted with ether. Add it to Na at pH=8.5 2 CO 3 In aqueous s...
Embodiment 2
[0022] 1. If the raw materials used are fresh rabbit brains, sheep brains, horse brains, donkey brains and other livestock animal brains, the drying method of the brains is the same as that of cow brains. If the raw materials used are the dried brain particles after extracting cholesterol, it can be directly carried out according to Example 1. Start from middle 2, the same below.
Embodiment 3
[0024] In step 2 of Example 1, adding ammonia water ethanol solution to the evaporated residue is to remove the lecithin and cephalin contained in the brain residue.
[0025] In this step, dehydrated alcohol with a volume of 4 times the volume can also be added to the brain slag, stirred and extracted for 4 hours, extracted 4 times, each time for 2 hours, and about 50% lecithin and about 25% brain can be extracted. Phospholipids and other phospholipids, but the main component is lecithin, which is conducive to the further purification of lecithin. Then use 4 times the amount of slag to extract with ammonia ethanol of PH=9, stir and extract 4 times, each 2 hours, contain cephalin in about 60% in the extract after drying, also contain 10% lecithin and other phospholipids, but The main component is cephalin, which is beneficial to the further separation of cephalin, and other steps are the same as in Example 1.
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Abstract
Phosphatidylserine is extracted from animal brains by: drying animal brains, smashing them into granules, putting propanone into the granules, stirring and extracting, separating propanone supernatant liquid contained cholesterine and oils from the brain granules, steaming the residues to be dried, adding n-hexane or alcohol or ammonia water alcohol into steamed dry residues, stirring and extracting, separating extracting liquid contained phospholipid compositions, steaming the residues again, putting ether into steamed dry residues, stirring, freezing and defreezing, filtering to remove deposits, removing the remained phospholipid further, adjusting supernatant liquid pH=3-6, steaming to remove ether, and steaming to dry crude phosphatidylserine again, dissolving crude phosphatidylserine dry powders in n-hexane, adding sodium acetate alcohol liquid, stirring, settling to be laminated, removing the supernatant contained inositol phospholipid, finally, steaming to dry the n-hexane in supernatant liquid to obtain high pure phosphatidylserine under reduced pressure.
Description
technical field [0001] The invention relates to a method for extracting phosphatidylserine from the brains of livestock animals, and is especially suitable for extracting phosphatidylserine from the brains of livestock animals such as cattle, sheep, rabbits, horses and donkeys. Background technique [0002] Bovine brain phosphatidylserine (PS) has the effects of enhancing memory and anti-senile dementia, which have been verified by animal experiments and clinical trials at home and abroad. 18(4), 207-230. However, it is difficult to extract and produce PS in batches using bovine brain as raw material. Lees M.B et al. (1981) and Abid et al. (1998) reviewed the chromatographic preparation and analysis methods of PS, and the preparation methods were extremely complicated. See Lees MB et al. preparation and Analysis of phosphatides Lipids and steroids (1981, 328-345) and Abed et al. Chromatography B 1998, (717) 279-293 (Abidi, SL et al. Journal of chromatography B, 717 (1988)...
Claims
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Application Information
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