Fluorescent in situ hybridization counting slide and counting method thereof
A fluorescent in situ hybridization and counting plate technology, which is applied in the field of fluorescent in situ hybridization detection, can solve the problems of inability to count cells accurately, achieve simple and accurate counting methods, and expand the application range
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Embodiment 1
[0031] 1. The diameter of a 40-fold mirror of a general microscope is 0.42mm, and the side length of its inscribed small square can be calculated to be 0.3mm. In this example, 256 fields of view are analyzed, and several slides are used. (Usually 6, 8, 10) square hybridization areas with a side length of 4.8mm, each large square hybridization area is divided into 256 small squares.
[0032] 2. Take out a certain amount from the sample to be tested and evenly smear it on a large square hybridization area of the gelatin-coated glass slide to perform fluorescence in situ hybridization.
[0033] 3. Use a fluorescence microscope to record the images in the 256 small squares in the hybridization area with image processing software (when recording, only the images in the small squares in each field of view or the entire field of view are recorded) , The image is processed later, only the image in the small square in the field of view is retained).
[0034] 4. Among the 256 images obtai...
Embodiment 2
[0037] 1. The diameter of a 100-fold mirror of a general microscope is 0.16mm, and the side length of its inscribed small square can be calculated to be 0.11mm. In this example, 400 fields of view are analyzed, and several slides are used. (Usually 6, 8, 10) square hybridization areas with a side length of 2.2 mm, and each large square hybridization area is divided into 400 small squares.
[0038] 2. Take out a certain amount from the sample to be tested and evenly smear it on a large square hybridization area of the gelatin-coated glass slide to perform fluorescence in situ hybridization.
[0039] 3. Use a fluorescence microscope to record the images in the 400 small squares in the hybridization area with image processing software (when recording, only the images in the small squares in each field of view or the entire field of view are recorded. , The image is processed later, only the image in the small square in the field of view is retained).
[0040] 4. Among the 400 images ...
Embodiment 3
[0043] 1. The diameter of a 100-fold mirror of a general microscope is 0.16mm, and the side length of its inscribed small square can be calculated to be 0.11mm. In this example, 600 fields of view are analyzed, and several slides are used. (Usually 6, 8, 10) side length is 3.3mm, width is 2.2mm (when n=600, ab=n has a 1 = 60, b 1 =10; a 2 =50, b 2 =12; a 3 = 30, b 3 = 20 and many other sets of solutions, we select the set of solutions that are most conducive to drawing, namely a 3 = 30, b 3 = 20) rectangular hybridization area, and each rectangular hybridization area is divided into 600 small squares.
[0044] 2. Take out a certain amount from the sample to be tested and evenly smear it on a certain rectangular hybridization area of the gelatin-coated glass slide to perform fluorescence in situ hybridization.
[0045] 3. Use a fluorescence microscope to record the images in the 600 small squares in the rectangle with image processing software (when recording, only the images in ...
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