Sensitivity-reinforced chemical light-emitting liquid containing auxiliary intensifier and intensifier
A technology of sensitization chemistry and auxiliary enhancement, applied in the field of chemiluminescent liquids, can solve the problem that the detection sensitivity cannot be further improved, and achieve the effects of low cost, obvious effect, and improved detection sensitivity.
Inactive Publication Date: 2007-05-30
BEIJING YUANDE BIO MEDICAL ENG
View PDF6 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
However, there is no additive or synergistic effect between these two types of enhancers, that is, when the two exist at the same time, the enhancement effect is only equivalent to the average value of the two, so the detection sensitivity cannot be further improved.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0009] The following is an embodiment of the invention, which is only used to illustrate the present invention, but the content of the present invention is not limited to the following
[0010] Example.
[0011] Add 1.25mMol / L luminol and 5mMol / LH 2 o 2 As the basic luminescent liquid, 20 μM mol / L 4-(4-hydroxybenzimino)antipyrine and 20 μM mol / L aminopyrine were successively added as enhancer and auxiliary enhancer, respectively. Then in the system, add different concentrations of horseradish peroxidase (HRP) and measure its luminous intensity as follows:
[0012] HRP photon count (RLU / s)
[0013] (pg)
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
This invention relates to a chemical luminescent solution used in chemical luminescent test to increase its flexibility, which is composed of a luminescent agent, an oxidant, reinforcement agents, an auxiliary reinforcement agent and a buffer solution.
Description
technical field [0001] The invention is a chemiluminescence liquid suitable for chemiluminescence detection to further improve sensitivity. Background technique [0002] The detection of biological macromolecules is known as an important part of modern biotechnology. They not only drive the rapid development of the whole biology, but also have been widely used in various fields of life science. However, there are many shortcomings in the radiolabeling and detection methods used in the past, such as: some radioactive isotopes are not conducive to storage and transportation due to their short half-life; at the same time, because the pollution of radioactive substances will cause certain harm to the health of workers and the environment, special equipment is required. Protection and waste treatment equipment; at the same time, the sensitivity of the method is also insufficient in some applications, which greatly limits their popularization. Therefore, people have been looking ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/07
Inventor 杨晓林孙旭东
Owner BEIJING YUANDE BIO MEDICAL ENG