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Method for production of compound Bacillus preparation by liquid fermentation

A compound preparation and Bacillus technology, applied in the direction of botany equipment and methods, bacteria, biocides, etc., can solve the problems of polluting environmental diseases, cross-infection, diseases, etc., to optimize the breeding ecological environment, reduce the degree of eutrophication, The effect of simple production process

Inactive Publication Date: 2003-03-12
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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Problems solved by technology

[0002] With the expansion of fishery production scale and domestic and foreign research on bacillus to improve aquaculture ecology, the use of bacillus in shrimp ponds can change the composition of bacteria in the pond, reduce the number of vibrio and increase the yield of shrimp; it can delay shrimp virus can effectively regulate water quality factors, but there are no clear standards for microbial preparations at home and abroad; there are also the following problems: (1) Environmental pollution and pathogen diversification increase the crisis of water quality
(2) The accumulation of a large number of metabolites produced during the breeding process causes the deterioration of the ecological environment of the pond and causes the occurrence of diseases
(3) Excessive use of drugs to prevent and control diseases reduces the quality of farmed products and affects food safety
(4) Disorderly discharge of aquaculture wastewater and silt, polluting the environment and easily causing cross-infection of diseases
These problems seriously restrict the sustainable development of fishery production

Method used

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  • Method for production of compound Bacillus preparation by liquid fermentation
  • Method for production of compound Bacillus preparation by liquid fermentation

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Effect test

Embodiment 1

[0009] Embodiment 1: (a) medium composition: corn steep liquor 3%, cornstarch 3%, peptone 3%, yeast extract 2%, micronutrient composition 8%, water 81% mix and dissolve; (b) submerged liquid fermentation, aeration Amount of 0.4V / N, fermentation temperature 30 ℃, fermentation time 18h; (c) porous mineral as carrier. (d) Centrifugal enrichment of bacteria; (e) Fluidized bed drying method, drying temperature 65°C, drying speed 300kg / h; (f) single strain fermentation, compound multi-strain preparation according to the characteristics of the ecological environment.

Embodiment 2

[0010] Embodiment 2: (a) medium composition: corn steep liquor 5%, cornstarch 1%, peptone 1%, yeast extract 0.5%, micronutrient composition 3%, water 88.5% mix and dissolve; (b) submerged liquid fermentation, aeration The concentration is 0.4V / V, the fermentation temperature is 37°C, and the fermentation time is 24h; (c) porous minerals are used as the carrier; (d) centrifugal enrichment of bacteria; The drying speed is 300kg / h; (f) single-strain fermentation, compound multi-strain preparation according to the characteristics of the ecological environment.

Embodiment 3

[0011] Embodiment 3: (a) culture medium composition: corn steep liquor 4%, cornstarch 2%, peptone 2%, yeast extract 1%, micronutrient composition 6%, water 86% mix and dissolve; (b) submerged liquid fermentation, aeration The concentration is 0.4V / V, the fermentation temperature is 35°C, and the fermentation time is 20h; (c) porous minerals are used as the carrier; (d) centrifugal enrichment of bacteria; The drying speed is 300kg / h; (f) single-strain fermentation, compound multi-strain preparation according to the characteristics of the ecological environment.

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Abstract

A process for preparing a composite bacillus liquid by fermentation is disclosed. It is characterized by that said product contains corn milk (3-5 wt.%), corn starch (1-3 wt.%), peptone (1-3 wt.%), yeast cream (0.5-2 wt.%), trace nutrients (3-8 wt.%) and water (80-90 wt.%). Its advantages are simple processing process, high purity of bacterial strain, and high effect on degradating organic pollutants in culturing fish.

Description

technical field [0001] The invention relates to a liquid fermentation production method of a bacillus compound preparation. Background technique [0002] With the expansion of fishery production scale and domestic and foreign research on bacillus to improve aquaculture ecology, the use of bacillus in shrimp ponds can change the composition of bacteria in the pond, reduce the number of vibrio and increase the yield of shrimp; it can delay shrimp virus It can effectively regulate water quality factors, but there are no clear microbial preparation standards at home and abroad; there are also the following problems: (1) Environmental pollution and pathogen diversification increase the crisis of water quality. (2) The accumulation of a large number of metabolites produced during the breeding process caused the deterioration of the ecological environment of the pond and caused the occurrence of diseases. (3) Excessive use of drugs to prevent and control diseases reduces the quali...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00C12N1/20
Inventor 李卓佳杨莺莺陈康德陈永青杨铿贾晓平
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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