Bacillus combination preparation liquid fermentation production method

A compound preparation and Bacillus technology, which is applied in the field of Bacillus compound preparation liquid fermentation production, can solve the problems of environmental pollution, cross-infection, diseases, etc., and achieves the optimization of the breeding ecological environment, reducing the degree of eutrophication, and eliminating harmful substances. Effect

Inactive Publication Date: 2010-06-16
XINYI DATANG FOOD
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Problems solved by technology

[0002] With the expansion of fishery production scale and domestic and foreign research on bacillus to improve aquaculture ecology, the use of bacillus in shrimp ponds can change the composition of bacteria in the pond, reduce the number of vibrio and increase the yield of shrimp; it can delay shrimp virus It can effectively control the water quality factors, but there are no clear standards for microbial preparations at home and abroad: There are also the following problems: (1) Environmental pollution and pathogen diversification increase the crisis of water quality
(2) The accumulation of a large number of metabolites produced during the breeding process causes the deterioration of the ecological environment of the pond and causes the occurrence of diseases
(3) Excessive use of drugs to prevent and control diseases reduces the quality of farmed products and affects food safety
(4) Disorderly discharge of aquaculture wastewater and silt, polluting the environment and easily causing cross-infection of diseases
These problems seriously restrict the sustainable development of fishery production

Method used

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  • Bacillus combination preparation liquid fermentation production method

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Experimental program
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Effect test

Embodiment 1

[0009] Embodiment 1: (a) medium composition: corn steep liquor 3%, cornstarch 3%, peptone 3%, yeast extract 2%, micronutrient composition 8%, water 81% mix and dissolve; (b) submerged liquid fermentation, aeration Amount 0.4V / V, fermentation temperature 30°C, fermentation time 18h: (c) Porous mineral as carrier. (d) Centrifugal enrichment of bacteria; (e) Fluidized bed drying method, drying temperature 65°C, drying speed 300kg / h; (f) single strain fermentation, compound multi-strain preparation according to the characteristics of the ecological environment.

Embodiment 2

[0010] Embodiment 2: (a) medium composition: corn steep liquor 5%, cornstarch 1%, peptone 1%, yeast extract 0.5%, micronutrient composition 3%, water 88.5% mix and dissolve; (b) submerged liquid fermentation, aeration The concentration is 0.4V / V, the fermentation temperature is 37°C, and the fermentation time is 24h; (c) porous minerals are used as the carrier; (d) centrifugal enrichment of bacteria; Drying speed 300kg / h: (f) single-strain fermentation, compound multi-strain preparation according to ecological environment characteristics.

Embodiment 3

[0011] Embodiment 3: (a) culture medium composition: corn steep liquor 4%, cornstarch 2%, peptone 2%, yeast extract 1%, micronutrient composition 6%, water 86% mix and dissolve; (b) submerged liquid fermentation, aeration The concentration is 0.4V / V, the fermentation temperature is 35°C, and the fermentation time is 20h; (c) porous minerals are used as the carrier; (d) centrifugal enrichment of bacterial cells: (e) adopt the fluidized bed drying method, the drying temperature is 70°C, and the The drying speed is 300kg / h; (f) single-strain fermentation, compound multi-strain preparation according to the characteristics of the ecological environment.

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Abstract

The invention relates to a bacillus combination preparation liquid fermentation production method. The bacillus combination preparation is composed of the following components (by weight percent): 3-5% of corn steep liquor, 1-3% of corn starch, 1-3% of peptone, 0.5-2% of yeast extract, 3-8% of micronutrient component and 80-90% of water. The invention has the following advantages: production technology is simple and convenient, feasibility is strong, product bacterial strain is pure, no organic matter is contained, organic pollution can be obviously degraded and eutrophication degree can be reduced when being used in fisheries production, and fungicide can timely degrade and convert metabolite produced in cultivation process, eliminate harmful substance, balance algae phase and bacteria phase and optimize cultivation ecological environment when being used in fishery environment.

Description

technical field [0001] The invention relates to a liquid fermentation production method of a bacillus compound preparation. Background technique [0002] With the expansion of fishery production scale and domestic and foreign research on bacillus to improve aquaculture ecology, the use of bacillus in shrimp ponds can change the composition of bacteria in the pond, reduce the number of vibrio and increase the yield of shrimp; it can delay shrimp virus It can effectively regulate water quality factors, but there are no clear standards for microbial preparations at home and abroad: There are also the following problems: (1) Environmental pollution and pathogen diversification increase the crisis of water quality. (2) The accumulation of a large number of metabolites produced during the breeding process caused the deterioration of the ecological environment of the pond and caused the occurrence of diseases. (3) Excessive use of drugs to prevent and control diseases reduces the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/07
Inventor 卢胜
Owner XINYI DATANG FOOD
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