Targeting-fusion DNA vaccine for caries-prevention, and its prepn. method

A DNA vaccine, anti-caries technology, used in recombinant DNA technology, DNA/RNA fragments, drug combinations, etc.

Inactive Publication Date: 2003-12-03
WUHAN UNIV
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  • Claims
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Problems solved by technology

However, the anti-caries DNA vaccine, like other DNA vacc...

Method used

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  • Targeting-fusion DNA vaccine for caries-prevention, and its prepn. method
  • Targeting-fusion DNA vaccine for caries-prevention, and its prepn. method
  • Targeting-fusion DNA vaccine for caries-prevention, and its prepn. method

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Embodiment Construction

[0070] The present invention will be described in further detail below according to accompanying drawing:

[0071] according to figure 1 , figure 2 It can be known that using professional molecular biology software: DNASIS comprehensive DNA sequence analysis software, Gene Construction kit 2.0 gene simulation cloning software, Omiga comprehensive molecular biology analysis software, BLAST homologous sequence analysis software, Oligo primer design analysis software and other software to analyze and design targets Fuse anti-caries DNA vaccine and simulate the whole process of cloning. Design and preparation of the plasmid pGCTLA carrying the CTLA4 target gene: Take 3ml of peripheral blood from healthy volunteer blood donors, anticoagulate with heparin, dilute with phosphate buffer saline (PBS) to equal times, and obtain mononuclear cell suspension by density gradient centrifugation with lymphocyte separation medium. After washing with PBS, adjust the cell concentration to 1×1...

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Abstract

A target fused DNA vaccine pGJA-P for preventing decayed tooth is prepared by using the PCR process through amplifying the antigen 4 signal peptide of toxic T lymphocyte, the sequence of extracellular region, and the genes in Ig gamma 1 hinge region and constant region, respectively cloning them to pU cm-T carrier to configune pGTLA and pJIg, linking them to configure their recombinant plasmid, verifying the correctness of inserted fragments, and inserting the fusion gene in the front end of pGLUA-P. Its advantages are simple process, high safety, low cost, and high effect to improve immunity.

Description

technical field [0001] The invention belongs to the technical field of oral preventive medicine, and more specifically relates to a sequence encoding human cytotoxic T lymphocyte antigen 4 signal peptide, extracellular region and Igγ 1 The DNA anti-caries vaccine of hinge region, constant region fusion gene, surface protein antigen gene and glucosyltransferase gene of Streptococcus mutans, and the invention also relates to a preparation method of the targeted fusion anti-caries DNA vaccine. Background technique [0002] Caries is a bacterial infectious disease. Many scholars have proved that Streptococcus mutans (Streptococcusmutans) is the most important pathogenic bacteria causing caries in humans. Glucosyltransferase (glucosyltransferaesGTF s ) and surface protein (surface protein antigen PAc) (also known as antigen B, I / II, IF, P1) are important cariogenic virulence factors of Streptococcus mutans. GTF s Synthesize glucan and mediate the dext...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61P1/02C12N15/11
Inventor 樊明文边专郭继华贾荣陈智彭彬范兵
Owner WUHAN UNIV
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