Biosensor for lactic acid in whole blood

A biosensor, blood lactic acid technology, applied in the field of biosensors, can solve the problems of interference of changes in oxygen content, poor anti-interference ability, poor redox characteristics, etc., to improve enzyme activity, improve response sensitivity, avoid blood The effect of a change in quantity

Inactive Publication Date: 2003-12-24
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The defect of this sensor is: the voltage added to the electrode leads is relatively high, which is 0.5v, and the anti-interference ability is not good. better response current
Chinese patent CN1219676A introduces a dispos

Method used

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  • Biosensor for lactic acid in whole blood
  • Biosensor for lactic acid in whole blood
  • Biosensor for lactic acid in whole blood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] A whole blood lactate biosensor such as figure 1 As shown, thick-film carbon electrodes 2 and silver electrode leads 2a were made on the PVC base plate 1 by screen printing technology, plastic film was covered around the carbon electrodes 2 to form a cavity 3, and 2μl concentration of 5mg / kg was injected into the cavity. ml CMC solution, the solution is dried in a 60°C oven to form a CMC layer 4a; add 2 μl of modified carbon nanotube suspension with a concentration of 2mg / ml, and after drying in a 60°C oven, a carbon nanotube layer 4b is formed. Then add 2 μl of mixed solution, the composition of which is 500 activity units / ml of L-lactate oxidase, 50 mg / ml of potassium ferricyanide, 20 mg / ml of seaweed, pH value of 6.0, and concentration of 0.2M phosphate buffer solution. The mixed layer 4c formed after drying in the drying oven is then covered with a plastic film on the cavity 2 to determine the volume of the cavity 3, which is 2 μl. The plastic film here is the cavit...

Embodiment 2

[0013] A whole blood lactate biosensor such as figure 1 As shown, thick-film carbon electrodes 2 and silver electrode leads 2a were made on PVC base plate 1 by screen printing technology, plastic film was covered around carbon electrodes 2 to form cavity 3, and 2μl concentration of 20mg / kg was injected into the cavity. ml of carboxymethyl cellulose CMC solution, the solution was oven-dried at 60°C to form a CMC layer 4a; add 2 μl of modified carbon nanotube suspension at a concentration of 6 mg / ml, and after oven-drying at 60°C, a carbon nanotube layer 4a was formed. Nanotube layer 4b, and then add 2μl of mixed solution, the ingredients are L-lactic acid oxidase 1000 activity units / ml, potassium ferricyanide 50mg / ml, bovine serum albumin 40mg / ml, pH value 7.0, the concentration is 0.4M phosphate buffer solution is dried in a drying oven to form a mixed layer 4c, and then a layer of plastic film is covered on the cavity 3 as the cavity cover 5, the volume of the cavity 3 is 2 μ...

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Abstract

A biosensor for measuring the content of lactic acid in whole blood features that an electrode is covered by plastic film to form a cavity, said plastic film has a capillary hole communicated with said cavity, and the reaction layer consisting of carboxymethyl cellulose, modified carbon nanotubes, and the mixture of L-lactate oxidase, reagent, stabilizer and buffering liquid is put in said cavity. Its advantages are high snesitivity, low voltage (300 mv), and less blood speciment without pretreatment.

Description

technical field [0001] The invention relates to a biosensor, in particular to a whole blood lactic acid biosensor. Background technique [0002] Whole blood lactic acid biosensor is a biosensor that uses biomolecules as sensitive elements to quickly, accurately and conveniently measure lactic acid content in blood and other fluids. Using this sensor to measure lactic acid content in human blood is used to reveal the severity of certain diseases It is used to guide diabetic patients to take Jiangtangling properly. Excessive use of Jiangtangling will cause glycolysis, increase the lactic acid content, cause acidosis, and endanger the life of the patient; it is used to measure the concentration of lactic acid in athletes, which can scientifically control the training intensity of athletes. The best training effect; In food science, the freshness of food can be determined by detecting the concentration of lactic acid. At present, the measurement of lactic acid mainly uses large...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/327
Inventor 陈裕泉吴维明童基均
Owner ZHEJIANG UNIV
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