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Detection method and quantitative analysis method for hydrogen radical

A detection method and free radical technology, which is applied in chemical method analysis, analytical materials, measuring devices, etc., can solve the problems of difficult detection sensitivity, low concentration of hydrogen free radicals, low detection, etc.

Inactive Publication Date: 2003-12-24
NIKHON TRIM KO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This is considered to be due to the low concentration of hydrogen radicals in electrolytically reduced water, making it difficult to detect with a method with low detection sensitivity

Method used

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  • Detection method and quantitative analysis method for hydrogen radical
  • Detection method and quantitative analysis method for hydrogen radical
  • Detection method and quantitative analysis method for hydrogen radical

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: detect the hydrogen free radical produced by platinum black-hydrogen with DBNBS

[0045] 1-1 Reagent

[0046] DBNBS is manufactured by Labo Tech Co., Ltd., platinum black is manufactured by Shifu Metal Mining Co., Ltd., and hydrogen is manufactured by Dayang Sanso Co., Ltd. Dissolve DBNBS in ultrapure water (Milli Q water) at a concentration of 2.5 mg / ml, store at 4°C, and use up within 2 weeks.

[0047] 1-2 Generation of hydrogen radicals from platinum black-hydrogen

[0048] The hydrogen gas becomes atomic hydrogen on the platinum surface. Then, hydrogen gas was bubbled at 45 ml / min into 20 ml of a 2.5 mg / ml DBNBS solution containing 0.01 mg / ml platinum black. After 5 minutes, 10 minutes, 15 minutes, 30 minutes, 45 minutes, and 60 minutes, 200 microliters were recovered, and after centrifugation at 12,000 rpm for 5 minutes, the supernatant was incubated in a constant temperature bath at 60°C for 1 hour. Use the material without blowing hydrogen gas a...

Embodiment 2

[0053] Example 2: Detection of hydrogen free radicals in electrolytically reduced water with DBNBS

[0054] 2-1 Reagents

[0055] The same substance as in Example 1 was used.

[0056] 2-2 Electrolyzed reduced water

[0057] Using the electrolytic reduction water device TI-8000 manufactured by Japan Trim Company, 0.01% NaCl water was electrolytically reduced at 4 levels (5A), and electrolytic reduced water was obtained on the cathode side.

[0058] 2-3 Detection of Hydrogen Free Radicals

[0059] Add 200 microliters of DBNBS preservation solution to 125 milliliters of electrolyzed reduced water, stir, and concentrate and dry in a constant temperature bath at 60° C. with a rotary evaporator. The concentrated dry matter was dissolved in 1 ml of ultrapure water (Milli Q water) and recovered. Then, it was incubated in a constant temperature bath at 60° C. for 1 hour, allowed to stand on ice for 5 minutes, and centrifuged at 12,000 rpm to obtain a supernatant. The same DBNBS sa...

Embodiment 3

[0061] Example 3: The relationship between the concentration of electrolyzed reduced water and the reaction value of hydrogen radicals, and the relationship between the strength of electrolysis and the reaction value of hydrogen radicals

[0062] It was tested whether there is a correlation between the concentration of electrolyzed reduced water and the amount of hydrogen radicals. The electrolytic reduced water was diluted with ultrapure water (Milli Q water) to measure the hydrogen radical reaction value of each sample. In order to investigate the relationship between electrolytic strength and hydrogen radical reaction value, water with NaCl concentration ranging from 0.0001% to 0.01% was prepared, and the active hydrogen reaction of water after electrolytic reduction was measured for each sample. The electrolytic reduced water is the reduced water obtained on the cathode side by 4-stage (5A) electrolytic reduction using the electrolytic reduced water device TI-8000 manufact...

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Abstract

A method for detecting a hydrogen radical in water or an aqueous solution which comprises adding a sodium salt of 3, 5-dibromo-4-nitrosobenzenesulfonic acid (DBNBS) to a sample and detecting a color caused by absorption characteristics of the resulting solution; a method for the quantitative analysis of the hydrogen radical which comprises blowing a hydrogen gas through a solution of 1, 1-diphenyl-2-picrylhydrazyl (DPPH) having an absorption near 517 nm and a solution of a sodium salt of 3, 5-dibromo-4-nitrosobenzenesulfonic acid (DBNBS) in the presence of a platinum black at a constant rate, and determining the concentration of a hydrogen radical using a calibration curve C in a graph illustrating the relationship between the absorbance of the absorption near 450 nm ascribed to a DBNBS azo compound and the concentration of a hydrogen radical.

Description

technical field [0001] The invention relates to a method for detecting hydrogen free radicals in water, especially electrolyzed reduced water or an aqueous solution, and a quantitative analysis method for the concentration of hydrogen free radicals. Background technique [0002] The so-called electrolytic reduced water obtained in the cathode chamber by electrolysis of an electrolyte solution such as sodium hydroxide has been considered to have the activity of eliminating active oxygen in the human body and the activity of inhibiting DNA strand cleavage. This is considered to be caused by the reducibility of active hydrogen contained in the electrolytically reduced water. [0003] Since the concentration of hydrogen free radicals contained in electrolytically reduced water has an impact on the activity of eliminating active oxygen in the human body, it is necessary to establish a detection method and a quantitative analysis method for hydrogen free radicals. [0004] "Activ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/78G01N21/80G01N31/00G01N31/22
CPCG01N21/80G01N21/31G01N21/78G01N21/783G01N31/22Y10T436/22G01N31/00
Inventor 白畑实隆大坪一道
Owner NIKHON TRIM KO LTD
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