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Sub-clone cell line mestastatic-human liver cancer and establishing method

A technology for liver cancer metastasis and liver cancer cells, applied in the field of animal cell lines, can solve the problems of lack of humoral and cellular immune functions, loss of T and B lymphocyte immune functions, etc.

Inactive Publication Date: 2005-01-12
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

SCID mice almost completely lose T and B lymphocyte immune function, lack humoral and cellular immune function

Method used

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  • Sub-clone cell line mestastatic-human liver cancer and establishing method
  • Sub-clone cell line mestastatic-human liver cancer and establishing method
  • Sub-clone cell line mestastatic-human liver cancer and establishing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for establishing a human liver cancer metastasis cell line, comprising the steps of:

[0036] (1) Inoculation of SCID mice: Get 5 SCID mice, male or female, with body weights of 13.1g, 14.3g, 14.4g, 15.2g, and 15.8g respectively, aged 4-7 weeks, and raised in a special pathogen-free environment. The human liver cancer cell H7402 in the logarithmic growth phase was made into a 0.2ml single-cell suspension containing 1×10 7 cells, seeded on Cs 137 γ-ray irradiated SCID rats subcutaneous in the axillae and back, SCID rats were provided by Tianjin Institute of Hematology, Chinese Academy of Medical Sciences;

[0037] (2) Confirmation of the metastatic site: the tumor-bearing SCID mice were killed by necking, and the pathological sections of each tissue were observed under a microscope to determine that the metastatic site was the lung;

[0038] (3) Primary culture: On the 66th day after inoculation of human hepatoma cell H7402, the tumor-bearing SCID mice were ki...

Embodiment 2

[0063] A method for establishing a human liver cancer metastasis cell line, comprising the steps of:

[0064] (1) Inoculation of SCID mice: take 4 SCID mice, male or female, weighing 13.4g, 13.6g, 14.3g, 15.8g respectively, aged 4-7 weeks, raised in a special pathogen-free environment, and will grow logarithmically 0.2ml single-cell suspension containing 1×10 7 cells, seeded on Cs 137 γ-ray irradiated SCID rats subcutaneous in the axillae and back, SCID rats were provided by Tianjin Institute of Hematology, Chinese Academy of Medical Sciences;

[0065] (2) Confirmation of the metastatic site: the tumor-bearing SCID mice were killed by necking, and the pathological sections of each tissue were observed under a microscope to determine that the metastatic site was the lung;

[0066] (3) Primary culture: On the 55th day after inoculation of human hepatoma cell H7402, tumor-bearing SCID mice were killed by necking, aseptically dissected, and the lungs were taken out for primary c...

Embodiment 3

[0070] A method for establishing a human liver cancer metastasis cell line, comprising the steps of:

[0071] (1) Inoculation of SCID mice: Take 3 SCID mice, male or female, weighing 14.4g, 15.3g, 15.7g, aged 4-7 weeks, raised in a special pathogen-free environment, and inoculate human liver cancer cells in logarithmic growth phase H7402 made into 0.2ml single cell suspension containing 1×10 7 cells, seeded on Cs 137 γ-ray irradiated SCID rats subcutaneous in the axillae and back, SCID rats were provided by Tianjin Institute of Hematology, Chinese Academy of Medical Sciences;

[0072] (2) Confirmation of the metastatic site: the tumor-bearing SCID mice were killed by necking, and the pathological sections of each tissue were observed under a microscope to determine that the metastatic site was the lung;

[0073](3) Primary culture: On the 67th day after inoculation of human hepatoma cell H7402, the tumor-bearing SCID mice were killed by necking, aseptically dissected, and th...

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Abstract

This invention disclose a human cancinoma of liver transfer subclone cell system and its set-up method in the following way: a human cancinoma of liver cell HT402 unicell suspension is inoculated into a SCID mouse and the transferred position it determined at lung part and by primary culture and secondary culture, the fetal calf serum concentration in the solution reduces to 10%, cell grows very well thus a cancinoma of liver transfer subclone cell system from parent human cancinoma of liver cell HT402 is set up matched with the parent cell, providing a new experiment material for its study.

Description

technical field [0001] The invention relates to the establishment of cancer cell lines and belongs to the field of animal cell lines. Background technique [0002] Liver cancer is one of the most common clinical malignant tumors, and its invasion, metastasis and recurrence after surgery are the main causes of death in patients. Liver cancer metastasis occurs early, and it is difficult to detect in the early stage of metastasis. Currently, there is still a lack of specific markers for liver cancer metastasis. In order to study the molecular mechanism of liver cancer metastasis, many scholars are committed to obtaining liver cancer cell lines with high metastasis tendency. The main method is to repeatedly inoculate nude mice with surgically resected patient's liver cancer tissue blocks, and then screen to obtain liver cancer cell lines with a high tendency to metastasize. However, this method takes a long period of time and cannot obtain paired cell lines that form a "child-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N13/00C12N15/08
Inventor 叶丽虹张晓东秦宵然朱惠芳王洪辉
Owner NANKAI UNIV
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