Method for freezing and storaging lymphocyte

A technology of lymphocytes and preservation methods, applied to the preservation of human or animal bodies, biochemical equipment and methods, microorganisms, etc., which can solve problems such as rejection and infection

Inactive Publication Date: 2006-01-04
宋清华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Using other people's materials can solve the problems of material aging and infirmity, but it may be infected by the

Method used

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Embodiment Construction

[0019] 1. The volunteer signs a consent form for the preservation of blood cells. The consent form stipulates that the volunteer's cells cannot be misappropriated without the volunteer's consent.

[0020] 2. Routine examination of the blood of volunteers. In order to ensure the quality of the cells, blood should be routinely checked before the cells are separated and cultured. These include: HIV test, hepatitis virus test, syphilis test, and other items that may affect cell quality and patient health.

[0021] 3. Collection of venous blood. With a syringe filled with heparin and an 18G needle, 5-100ml of blood is drawn from the vein of the volunteer in the usual way. The amount of heparin depends on the amount of blood taken.

[0022] 4. Blood processing. After sterilizing the blood-filled syringe with alcohol, place it in a sterile operating table, and gently pour the blood into a centrifuge tube or a suitable container. The collected blood is diluted with the same amount of norm...

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PUM

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Abstract

The cell separating and storing method is to take healthy and active cell out of body and to freeze store after separation, activation and proliferation, so as to provide effective, safe and reliable disease preventing and treating material. Specifically, the lymphocyte freezing and storing method includes the steps of: for the donor to apply, to exam the blood of the donor, to collect venous blood, to separate lymphocyte from venous blood via a density centrifuging process, to activate and culture lymphocyte, to collect active lymphocyte, to freeze and store lymphocyte, and to defreeze before use.

Description

Technical field [0001] The invention relates to a method for separating and storing lymphocytes. Background technique [0002] The current cell therapy, biological therapy is to use the patient's current materials, such as cells, tissues, etc., and even use other people's materials for treatment. This method has some disadvantages. For example, the current patient is a patient, and of course his physical condition will be reflected in the treatment materials used. Cancer patients’ cells may have low killing activity against cancer cells, virus-infected patients may have lymphocytes infected, and elderly patients’ bodies, tissues, and cells may have aged. After the cancer patient undergoes surgery, radiation therapy, or chemotherapy, the body is already very weak, and the cells may have been severely damaged in quantity and quality. If you prepare for biological treatment after treatment with other methods, it may take a considerable amount of time and delay the timing of treatmen...

Claims

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Application Information

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IPC IPC(8): C12N5/08A61K35/12A01N1/02A61K35/17
Inventor 宋清华
Owner 宋清华
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