Method for freezing and storaging lymphocyte
A technology of lymphocytes and preservation methods, applied to the preservation of human or animal bodies, biochemical equipment and methods, microorganisms, etc., which can solve problems such as rejection and infection
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[0019] 1. The volunteer signs a consent form for the preservation of blood cells. The consent form stipulates that the volunteer's cells cannot be misappropriated without the volunteer's consent.
[0020] 2. Routine examination of the blood of volunteers. In order to ensure the quality of the cells, blood should be routinely checked before the cells are separated and cultured. These include: HIV test, hepatitis virus test, syphilis test, and other items that may affect cell quality and patient health.
[0021] 3. Collection of venous blood. With a syringe filled with heparin and an 18G needle, 5-100ml of blood is drawn from the vein of the volunteer in the usual way. The amount of heparin depends on the amount of blood taken.
[0022] 4. Blood processing. After sterilizing the blood-filled syringe with alcohol, place it in a sterile operating table, and gently pour the blood into a centrifuge tube or a suitable container. The collected blood is diluted with the same amount of norm...
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