Polyepitope DNA vaccine of anti-simple herpes virus-2 infection and its preparing method

A DNA vaccine, multi-epitope technology, applied in the fields of medicine and genetic engineering

Inactive Publication Date: 2006-03-22
FUDAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most genital herpes are caused by HSV-2, which can be a chronic recurrence process, and there is no cure for it

Method used

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  • Polyepitope DNA vaccine of anti-simple herpes virus-2 infection and its preparing method
  • Polyepitope DNA vaccine of anti-simple herpes virus-2 infection and its preparing method
  • Polyepitope DNA vaccine of anti-simple herpes virus-2 infection and its preparing method

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Embodiment Construction

[0018] Embodiments of the present invention are further described below.

[0019] 1. Primer design: refer to [ figure 1 】Designed 6 pairs of primers, using the whole human HSV-2 viral genome DNA as a template, designed primers (including enzyme cutting sites) to amplify the main immunodominant segments and ICP27 fragments of the three envelope glycoproteins gD, gB and gC respectively . See [Table 1] for primer sequences:

[0020] 2. Segmental cloning recovery of antigen target genes: take 2ul HSV virus lysate, and use Taqplus high-fidelity DNA polymerase to perform PCR amplification on the 6 selected antigen genes respectively. The conditions are as follows:

[0021] 50μl PCR amplification reaction system: ddH 2 O 9.5 μl

[0022] 10×Buffer 5.0μl

[0023] 4×dNTP (10mM) 1.0μl

[0024] Primer F (10μM) 1.0μl

[0025] Primer R (10μM) 1.0μl

[0026] Template 2...

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Abstract

The present invention belongs to the field of medicine and gene engineering technology, and is especially one kind of polyepitope DNA vaccine resisting herpes virus-2 infection and its preparation process. The present invention connects gB, gC and gD of HSV-2 and the main antigen epitope region plus interval sequence of immediate early protein of ICP27 serially to eukaryotic expression vector pcDNA3.1 to obtain the DNA vaccine HV-pcDNA3.1. The DNA vaccine may be used in preventing and treating HSV-2 infected diseases, such as genital herpes, etc. The DNA vaccine has the features of high specificity, high protection and simple preparation process.

Description

technical field [0001] The invention belongs to the technical field of medicine and genetic engineering, in particular to a new anti-HSV-2 multi-epitope DNA vaccine and its application Background technique [0002] Herpes simplex virus (HSV) infection is a relatively common infectious disease in humans. HSV-1 mainly causes non-genital infections such as cold sores, gingivostomatitis, encephalitis, and keratoconjunctivitis, while HSV-2 causes genital infections. Most genital herpes are caused by HSV-2, which can present a chronic recurrence process, and there is no cure for it yet. The prevalence of the disease in the world is very obvious, accounting for about 10% of sexually transmitted diseases. The public health problems caused by HSV-2 infection have prompted people to develop vaccines that can prevent and control infection. Studies have shown that compared with conventional inactivated vaccines, attenuated vaccines, and subunit vaccines, DNA vaccines can not only indu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K39/245A61P31/22
Inventor 朱乃硕徐金华许炎夏天郑志忠
Owner FUDAN UNIV
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