Gen-seng saponin Rg2 preparation method, its pharmaceutical composition and uses in pharmacy

A technology of ginsenosides and lower alkanols, which is applied in the directions of drug combinations, steroids, and medical preparations containing active ingredients, etc. It is not suitable for large-scale production and other problems, and achieves the effect of good treatment of dementia, shortening sleep time, and regulating brain nerve cell apoptosis.

Inactive Publication Date: 2006-04-26
吉林省中医中药研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The main disadvantages of silica gel column chromatography are that the amount of separation on each column is small, the separation speed is slow, and a large amount of toxic organic solvents are used, resulting in high cost, low yield, unsafe, and not suitable for large-scale production
Although the preparative HPLC method can separate high-purity ginsenoside Rg 2 , but the equipment is expensive, costly, and the amount of methanol or acetonitrile is too large, so the cost increases accordingly
In conclusion, the existing ginsenoside Rg 2 The preparation method is not suitable for large-scale production

Method used

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  • Gen-seng saponin Rg2 preparation method, its pharmaceutical composition and uses in pharmacy
  • Gen-seng saponin Rg2 preparation method, its pharmaceutical composition and uses in pharmacy
  • Gen-seng saponin Rg2 preparation method, its pharmaceutical composition and uses in pharmacy

Examples

Experimental program
Comparison scheme
Effect test

experiment Embodiment 1

[0044] Experimental Example 1: Ginsenoside Rg 2 Extraction and separation method

[0045] Take 100kg of ginseng leaf meal, decoct three times in water for 3 hours each time, combine the decoctions, filter, concentrate the filtrate, add 3 times the amount of 95% ethanol to precipitate impurities, discard the sediment, decolorize with activated carbon, and recover ethanol. After the ethanol extract was dissolved in 10 times of water, ammonium sulfate (agricultural fertilizer) or sodium chloride (edible salt) was added to make the solution concentration reach saturation, and static precipitation was carried out for 8 hours. Take the precipitate, dissolve it in water again, carry out gradient (5%, 10%, 15%, 20%, 30% concentration) salting-out with sodium chloride or ammonium sulfate, respectively, and collect the precipitate respectively. Due to the different amount of solute in the solution, the gradient salting-out concentration is different. Therefore, under thin-layer chroma...

experiment Embodiment 2

[0059] Experimental Example 2: Ginsenoside Rg 2 anti-dementia effect

[0060] (1) Ginsenoside Rg 2 Injection of amyloid (Aβ 1-40 ) caused by memory impairment

[0061] The experiment used Alzheimer's dementia (AD) model. Take 70 rats, divide them into 7 groups, and anesthetize them with chloral hydrate. Except for the sham operation group, the other groups are injected with Aβ in the CA1 region of the hippocampus. 1-40 (4 μg) and ibotenic acid (1 μg) in a total of 1 μL. After the model was established, the test drugs were given the doses shown in Table 1.1 ip, and the model group was given the same volume of normal saline ip, once a day, for a total of 7 days. The test drug used is a single configuration C-20 (S) type ginsenoside Rg 2 , single configuration C-20 (R) type ginsenoside Rg 2 And Mixed C-20(SR) Ginsenoside Rg 2 (The following experiments are referred to as S, R, SR for short). On the 6th day of administration, a Y-type electric maze test was performed to o...

experiment Embodiment 3

[0094] Experimental Example 3: Antidepressant and Central Exciting Effects

[0095] (1) Effects on the contents of NE, DA, and 5-HT in rat brain tissue

[0096] Commonly used models of depression use drugs or stress stimulation to reduce the content of NE, DA, and 5-HT in brain tissue, causing central depression. In this experiment, cerebral ischemia-reperfusion method was used to reduce the content of NE, DA and 5-HT in brain tissue.

[0097] 56 rats were taken, divided into 7 groups, and anesthetized. Except for the sham operation group, each rat was introduced into the internal carotid artery with a suture (4-0 polyethylene line) from the left external carotid artery, and the middle cerebral artery (MCA) was blocked. ). The timing starts from blocking the blood flow, and after 0.5 hours, pull the tie line and reperfuse. Animals in each group received the test drugs ip at the dose shown in Table 4.1 on the operation day, postoperative day 1, day 2, day 3, and day 4, and t...

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Abstract

The present invention relates to kind one ginsenoside Rg2 preparing process suitable for industrial production, and gradient salt separation process with simple operation is used in substituting traditional silicon gel chromatography process. The present invention also relates to medicine composition with ginsenoside Rg2 as active component and its application in medicine for preventing and treating dementia, depression, peripheral circulation disturbance and related diseases.

Description

technical field [0001] The present invention relates to ginsenoside Rg 2 The preparation method, the pharmaceutical composition with the compound as an active ingredient, and their application in medicines for preventing or treating dementia, depression, peripheral microcirculation disturbance and other related diseases. Background technique [0002] There are many kinds of ginsenosides in ginseng, and there are more than 30 kinds of ingredients that have been clearly identified, among which ginsenoside Rg 2 (Ginsegnoside Rg 2 ) belongs to protopanaxatriol glycosides which were isolated and named in the early years. Studies have shown that during the processing of red ginseng, the C-20 structure of ginsenoside is unstable, and the sugar is easily hydrolyzed and replaced by hydroxyl to form ginsenoside C-20 (S) or C-20 (R) isomers. Ginsenoside Rg in red ginseng 2 The content of C-20(RS) is significantly higher than that of raw sun-dried ginseng, but it is not used to extr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J17/00A61K31/7048A61P9/00A61P25/24A61P25/28
Inventor 李龙云田建明金毅
Owner 吉林省中医中药研究院
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