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Pseudo-tissue for quality control and quality control method using same

A technology of precision management and organization, applied in material inspection products, biological testing, etc., can solve the problems of different degree of homogenization of excised tissue, wear consumption, different degree of homogenization of excised tissue, etc.

Inactive Publication Date: 2006-04-26
SYSMEX CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When homogenizing manually, the degree of homogenization of resected tissues varies due to the different skill levels of those who perform the pretreatment
In addition, when automatic homogenization is performed with an automatic crushing device, etc., the degree of homogenization of the resected tissue may vary due to the homogenization conditions such as the rotation number setting of the mixer or the wear and tear of the mixer due to continuous use.

Method used

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  • Pseudo-tissue for quality control and quality control method using same
  • Pseudo-tissue for quality control and quality control method using same
  • Pseudo-tissue for quality control and quality control method using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Pretreatment accuracy management using absorbance measurement, including the following steps: Performing homogenization on suspected tissues for accuracy management with a predetermined indication value, which is composed of nucleic acid or cells and a support capable of retaining nucleic acid or cells. The steps of measuring the nucleic acid concentration of the sample thus obtained during the nucleic acid extraction treatment included therein; and the step of comparing the measured value of the nucleic acid concentration with the above indicated value, and judging whether the above nucleic acid extraction treatment is appropriate.

[0052]

[0053] First, the representation value is calculated.

[0054] The indicated value is the measured value of the nucleic acid concentration calculated by performing the predetermined pretreatment on the suspected tissue prepared by the determined operation procedure and measuring the absorbance thereof. By comparing the indicated...

Embodiment 2

[0086]The pretreatment accuracy management by nucleic acid amplification and nucleic acid detection includes the following steps: homogenate is composed of target nucleic acid or cells containing target nucleic acid, and a support capable of retaining target nucleic acid or cells containing target nucleic acid, with An amplification process of amplifying the target nucleic acid contained in the sample obtained by amplifying the suspected tissue for accuracy management of a predetermined indicated value; a measurement process of detecting the amplified target nucleic acid and measuring the concentration of the target nucleic acid from the target suspected tissue; and A process of comparing the measured value and the indicated value of the target nucleic acid concentration of the suspected tissue, and judging whether the homogenization, amplification process, and measurement process are appropriate.

[0087]

[0088] Next, the preparation of the pseudo tissue γ used to calculat...

Embodiment 3

[0127] In Example 3, the operating procedure and device operation in nucleic acid detection using GD-100 (manufactured by SYSMEX) will be specifically described. Here, the case of using a pseudo tissue composed of cells and a holder holding the cells will be described.

[0128]

[0129] Before the nucleic acid amplification and nucleic acid detection of the suspected tissue, first, a cell suspension containing cells thought to contain CK19 mRNA was mixed with a homogenization reagent to prepare a sample, homogenized sufficiently, and used as a reference sample ε. Also, pre-count the number of cells in the cell suspension.

[0130] Then, using the same amount of the same substance as the cell suspension used to prepare the reference sample ε, the pseudo tissue ε was prepared in the same manner as the above-mentioned preparation of the pseudo tissue α.

[0131] Add homogenization reagent to suspected tissue ε. Adjust the amount of the homogenization reagent added so that the...

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Abstract

The present invention provides a suspected tissue for precision management that can be used for pretreatment precision management in genetic testing. The quasi-tissue used for precision management is composed of nucleic acid or cells, and a gel capable of retaining the nucleic acids or cells.

Description

technical field [0001] The present invention relates to a quasi-organization for accuracy management (or called "simulated organization for accuracy management", hereinafter also referred to as "suspicious organization" separately) and an accuracy management method using the same. Background technique [0002] In recent years, genetic testing has spread rapidly in the field of clinical diagnosis. Genetic examination is an examination for the clinical purpose of analyzing nucleic acid or chromosomes, etc., to check whether there are mutations or karyotypes related to genetic diseases. Examining tissue excised from an organism for the presence of nucleic acid from tumor cells is an example of genetic testing. The inspection process mainly consists of three processes: pretreatment, nucleic acid amplification and detection. [0003] There are various methods of preprocessing. For example: First, a reagent for pretreatment is added to a tissue excised from a living body which ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/50
Inventor 山崎正稔藤本敬二
Owner SYSMEX CORP