Organic phosphorus pesticide bionic identification test reagent kit
A technology of organophosphorus pesticides and detection kits, which is applied in biological testing, material inspection products, chemiluminescence/bioluminescence, etc., can solve the problems of increased difficulty in prevention and control, increased dosage, and low sensitivity, and achieves a wide range of applications, Effects with fewer constraints and high sensitivity
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Embodiment 1
[0035] Using ethyl parathion as a template molecule, a molecular probe with bionic recognition and detection functions for parathion ethyl was prepared, and a corresponding kit was prepared.
[0036] a. Preparation of coordination complex
[0037] (1) 0.2mmol Eu in the reactor 2 o 3 Dissolve in water, add 65% nitric acid with a reaction ratio to obtain a clear europium nitrate solution;
[0038] (2) choose template molecule to be that ethyl parathion 0.2mmol is dissolved in the mixed solution that 30mL methanol / water volume ratio is 1 / 1, stirs to the functional monomer vinyl benzoate 0.6mmol of reaction proportion after fully dissolving, After the functional monomer is completely dissolved, add the aforementioned europium nitrate solution to obtain solution A;
[0039] (3) Adjust the solution A to a pH value of 9 with 2mol / L sodium hydroxide solution, then stir for 4 hours, the solution becomes slightly yellow, and flocs appear;
[0040] (4) Cover the reactor in (3) with a...
Embodiment 2
[0052] Using methyl parathion as a template molecule, a molecular probe with bionic recognition and detection functions for parathion was prepared, and a corresponding kit was prepared.
[0053] a. Preparation of coordination complex
[0054] (1) 0.2mmol Eu in the reactor 2 o 3 Dissolve in water, add 28% nitric acid with a reaction ratio to obtain a clear europium nitrate solution;
[0055] (2) Dissolve 0.2mmol of the template molecule methyl parathion in 30mL of a mixed solution with a methanol / water volume ratio of 5 / 1, stir until completely dissolved and add 0.6mmol of the functional monomer propylene benzoate, and the functional monomer is completely dissolved Then add the aforementioned europium nitrate solution to obtain solution A;
[0056] (3) 1mol / L sodium hydroxide solution adjusts the pH value of solution A to 10 to obtain a clear solution; stir the mixed solution for 60 hours until the solution becomes slightly yellow and flocs appear;
[0057] (4) Cover the re...
Embodiment 3
[0069] Using malathion as a template molecule, molecular probes with biomimetic recognition and detection functions for malathion were prepared, and corresponding kits were prepared.
[0070] a. Preparation of coordination complex
[0071] (1) 0.2mmol Eu in the reactor 2 o 3 Dissolve in water, add 35% nitric acid with a reaction ratio to obtain a clear europium nitrate solution;
[0072] (2) choose template molecule to be malathion 0.2mmol and be dissolved in 30mL methanol / water volume ratio is 1 / 1 in the mixed solution, stir to dissolve completely after adding the functional monomer vinyl benzoate 0.6mmol of reaction ratio, function After the monomer is completely dissolved, add the aforementioned europium nitrate solution to obtain solution A;
[0073] (3) The pH value of solution A is adjusted to 8.5 with 1mol / L sodium hydroxide solution, and after stirring for 24 hours, the solution becomes slightly yellowish green, and flocs appear;
[0074] (4) Cover the reactor in (...
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