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Organic phosphorus pesticide bionic identification test reagent kit

A technology of organophosphorus pesticides and detection kits, which is applied in biological testing, material inspection products, chemiluminescence/bioluminescence, etc., can solve the problems of increased difficulty in prevention and control, increased dosage, and low sensitivity, and achieves a wide range of applications, Effects with fewer constraints and high sensitivity

Inactive Publication Date: 2006-05-17
THE FIRST INST OF OCEANOGRAPHY SOA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technique involves combining two techniques - one called molecular enzyme immune-affinity identification (MEI) and another called Fluorescent Analysis Method(FAC). These methods combine both technologies for creation of specific binding agents that can be used effectively when needed or stored safely at room temperature. They allow researchers to study how these materials work together more efficiently than just identifying them themselves. By analyzing their properties through this process they are able to identify unknown substances like DNA fragments from other organisms without being limited by environmental factors such as light levels or pH values.

Problems solved by technology

This patent describes various technical problem addressed during this patented study on pesticidation agents currently being applied worldwide due to issues like cost overrunments, negative impact on ecologically friendly species, potential health hazards associated therewith, and waste disposal concerns. Current techniques involve extracting pesticidal residue from plants and other sources, treating these residual matters into nontoxic forms, applying chemical fertilizers, controlling crop rotation, developing disease resistance mechanisms, etc., making the processes cumbersome and requiring highly trained labor force operators. Therefore, the inventors aim to provide efficient solutions without compromizing beneficial effects while reducing environmental risks involved in pesticiding activities.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Using ethyl parathion as a template molecule, a molecular probe with bionic recognition and detection functions for parathion ethyl was prepared, and a corresponding kit was prepared.

[0036] a. Preparation of coordination complex

[0037] (1) 0.2mmol Eu in the reactor 2 o 3 Dissolve in water, add 65% nitric acid with a reaction ratio to obtain a clear europium nitrate solution;

[0038] (2) choose template molecule to be that ethyl parathion 0.2mmol is dissolved in the mixed solution that 30mL methanol / water volume ratio is 1 / 1, stirs to the functional monomer vinyl benzoate 0.6mmol of reaction proportion after fully dissolving, After the functional monomer is completely dissolved, add the aforementioned europium nitrate solution to obtain solution A;

[0039] (3) Adjust the solution A to a pH value of 9 with 2mol / L sodium hydroxide solution, then stir for 4 hours, the solution becomes slightly yellow, and flocs appear;

[0040] (4) Cover the reactor in (3) with a...

Embodiment 2

[0052] Using methyl parathion as a template molecule, a molecular probe with bionic recognition and detection functions for parathion was prepared, and a corresponding kit was prepared.

[0053] a. Preparation of coordination complex

[0054] (1) 0.2mmol Eu in the reactor 2 o 3 Dissolve in water, add 28% nitric acid with a reaction ratio to obtain a clear europium nitrate solution;

[0055] (2) Dissolve 0.2mmol of the template molecule methyl parathion in 30mL of a mixed solution with a methanol / water volume ratio of 5 / 1, stir until completely dissolved and add 0.6mmol of the functional monomer propylene benzoate, and the functional monomer is completely dissolved Then add the aforementioned europium nitrate solution to obtain solution A;

[0056] (3) 1mol / L sodium hydroxide solution adjusts the pH value of solution A to 10 to obtain a clear solution; stir the mixed solution for 60 hours until the solution becomes slightly yellow and flocs appear;

[0057] (4) Cover the re...

Embodiment 3

[0069] Using malathion as a template molecule, molecular probes with biomimetic recognition and detection functions for malathion were prepared, and corresponding kits were prepared.

[0070] a. Preparation of coordination complex

[0071] (1) 0.2mmol Eu in the reactor 2 o 3 Dissolve in water, add 35% nitric acid with a reaction ratio to obtain a clear europium nitrate solution;

[0072] (2) choose template molecule to be malathion 0.2mmol and be dissolved in 30mL methanol / water volume ratio is 1 / 1 in the mixed solution, stir to dissolve completely after adding the functional monomer vinyl benzoate 0.6mmol of reaction ratio, function After the monomer is completely dissolved, add the aforementioned europium nitrate solution to obtain solution A;

[0073] (3) The pH value of solution A is adjusted to 8.5 with 1mol / L sodium hydroxide solution, and after stirring for 24 hours, the solution becomes slightly yellowish green, and flocs appear;

[0074] (4) Cover the reactor in (...

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PUM

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Abstract

The present invention relates to an organophosphorous pesticide bionic identification detection kit. Said kit is formed from polystyrene enzyome-linked plate with 96 wells, correspondent organophosphorous pesticide bionic identification molecular probes and organophosphorous pesticide solution with standard concentration. It is characterized by that on the polystyrene enzyme-linked plate with 96 wells the molecular probes with bionic identification and detection functions are coated. Said invention also provides the concrete steps of its preparation method. Said invention fully utilizes the combination of molecular engram technique and fluorescence analysis technique to greatly raise detection capability for organophosphorous pesticide.

Description

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Claims

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Application Information

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