Solid composite microbe microsphere for soil rehabilitation and its preparation method

A technology for composite microorganisms and soil remediation, which is applied in the field of solid composite microbial microspheres for soil remediation and its preparation, and can solve the problems of hindering the diffusion of substrates and oxygen, and the inapplicability of macromolecular substrates.

Active Publication Date: 2006-06-21
CHINA PETROLEUM & CHEM CORP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

On the other hand, in the process of cell immobilization application, since the embedding material hinders the

Method used

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  • Solid composite microbe microsphere for soil rehabilitation and its preparation method
  • Solid composite microbe microsphere for soil rehabilitation and its preparation method

Examples

Experimental program
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Example Embodiment

[0022] Example 1

[0023] 1) Plane culture: Cunninghamia chinensis and Pseudomonas are used as embedded fungi and bacteria. Use microbiological methods to prepare potato flat medium (PDA) and LB medium, inoculate Cunninghamella chinensis on the prepared PDA flat medium, culture at 28°C for 4 days, and store it for later use; Pseudomonas Inoculate on the prepared LB flat medium, culture at 30°C for 2 days, and store for later use;

[0024] 2) Shake flask culture: Prepare 100ml of PDA liquid medium and 100ml of LB liquid medium according to the microbiological method, respectively put them into 300ml Erlenmeyer flasks, sterilize at 121℃ for 15 minutes, cool to room temperature, and then Inoculate Cunninghamella chinensis and Pseudomonas in a sterile workbench. Place the shake flask in an air shaker. The fungal culture condition is 28℃, 160rpm, culture for 4 days; the bacterial culture condition is 30℃, 160rpm culture. 1 day; collect the bacterial liquid, mix with the volume ratio of...

Example Embodiment

[0029] Example 2

[0030] The difference between Embodiment 2 and Embodiment 1 is:

[0031] 1) Plane culture: Cunninghamella echinensis, Phanerochaete chrysosporium and Rhodococcus are used as embedded fungi and bacteria. Prepare potato flat medium (PDA) and LB medium by microbiological methods, inoculate fungi on the prepared PDA flat medium, cultivate at 25°C for 5 days, and store for later use; inoculate bacteria into the prepared LB flat medium for cultivation Basically, incubate at 30°C for 2 days and store for later use.

[0032] 2) Shake flask culture: Prepare 200ml of PDA liquid medium and 100ml of LB liquid medium according to the microbiological method, put them into 300ml Erlenmeyer flasks, sterilize at 121℃ for 15 minutes, cool to room temperature, and then Inoculate Cunninghamella echinensis, Phanerochaete chrysosporium, and Rhodococcus into the aseptic workbench. Place the shake flask in an air shaker. The fungus culture condition is 25℃, 160rpm, culture for 5 days; ...

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Abstract

The present invention provides one kind of soil repairing solid compound microbe microball and its preparation process. The soil repairing solid compound microbe microball is prepared with calcium alginate as embedding material and solid calcium carbonate as solid pore-creating agent to embed petroleum hydrocarbon degrading fungi, bacteria and nutritive matter, and the preparation process includes suspension preparation, microball formation, eliminating pore-creating agent and other steps. The present invention has the advantages of solid carrier maintained, has super large pores for reinforced mass transfer and embedded fungi, bacteria and nutritive matter, and thus possesses the advantages of high mass transferring speed, protection, nourishing, high activity and stability, etc. This kind of solid compound microbe microball may find its wide application in the biological repair of petroleum polluted soil.

Description

technical field [0001] The invention relates to a solid composite microbial microsphere for soil restoration and a preparation method thereof, which belongs to the preparation technology of microbial preparations for bioremediation of petroleum-contaminated soil. Background technique [0002] Environmental bioremediation technology is mainly composed of three aspects: (1) the technology of utilizing the metabolic ability of indigenous microorganisms (Natural Attenuation); (2) the method of activating the decomposition ability of indigenous microorganisms (Biostimulation, referred to as biological activation method); (3) adding A method of specific microorganisms (groups) with the ability to decompose refractory compounds at a high speed (Bioaugmentation, referred to as biological addition method). For the bioremediation process of petroleum-contaminated soil, due to the complex composition, biodegradability and low bioavailability of petroleum pollutants, it is difficult for...

Claims

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Application Information

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IPC IPC(8): B09C1/10C12P1/02C12P1/04C12S99/00
Inventor 刘铮韩慧龙周鑫闫明张敏莲王福远张坤
Owner CHINA PETROLEUM & CHEM CORP
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