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Biomagnetism nano target anti-cancer drug and its preparation

A bio-nano, anti-cancer drug technology, used in anti-tumor drugs, drug combinations, pharmaceutical formulations, etc., can solve the problems of low drug loading, high toxicity, and weak targeting

Active Publication Date: 2007-03-14
南京英睿杰生化科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to address the demand for targeted therapy of tumors and solve the common problems of low drug loading, poor stability, high toxicity and weak targeting of artificially synthesized nano-magnetic drug carriers at present. A natural resource with nanoscale and stable crystal form is used as a drug carrier, and a drug carrier with appropriate particle size and shape, high drug loading and drug encapsulation efficiency, good biocompatibility, easy degradation, low toxicity, and stability Nano-magnetic targeted drug with high anti-cancer function and bio-nano magnetosome drug loading method

Method used

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  • Biomagnetism nano target anti-cancer drug and its preparation
  • Biomagnetism nano target anti-cancer drug and its preparation
  • Biomagnetism nano target anti-cancer drug and its preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Embodiment 1 physical adsorption method preparation biological magnetosomes carrying doxorubicin

[0069] Experimental steps:

[0070] Mix the anticancer drug doxorubicin solution and the biological nano-magnetosome suspension, stir or intermittently pulse ultrasound for 0.5-24 hours, and place it at 4°C overnight; use a magnet to absorb the magnetosomes, and wash with pure water or buffer solution Several times, and then use a magnet to absorb the magnetosomes to obtain the product.

[0071] Experimental results:

[0072] It can be seen from the results in Table 1 that:

[0073] (1) Using the direct physical adsorption method, the biological nano-magnetosomes (BMP) have a high drug loading capacity for doxorubicin (AMS), and the maximum in this experiment can reach 11.07×10 3μgADM / mgBMP, while the content of doxorubicin in the artificially synthesized ferrite magnetic nanoparticles is 57.5μg, it can be seen that the drug loading capacity of biological nano magnetoso...

Embodiment 2

[0078] Example 2 Preparation of Biomagnetosomes Carrying Doxorubicin by Direct Coupling and Step-by-Step Method

[0079] Experimental steps:

[0080] Add coupling agent to the magnetosome suspension, mix well, stir or pulse ultrasonic reaction intermittently for 0.5-24 hours, the reaction product is sucked out with a magnet, washed several times to remove unreacted coupling agent, and then adsorbed with a magnet . Mix the doxorubicin solution with the above-mentioned magnetosomes modified by the coupling agent, stir or pulse ultrasound repeatedly and intermittently, and react for 0.5-24 hours. The reaction product is adsorbed by a magnet to remove unreacted anti-cancer chemical drugs, pure water or phosphate buffer solution to wash several times, and then use a magnet to suck out the magnetosomes to obtain the product.

[0081] Experimental results:

[0082] It can be seen from the results in Table 2 that:

[0083] (1) By adopting the direct coupling step-by-step method, t...

Embodiment 3

[0088] Example 3 Direct Coupling One-Step Preparation of Biological Magnetosomes Carrying Doxorubicin

[0089] Experimental steps:

[0090] The bionano magnetosome suspension is mixed with the coupling agent aqueous solution and the anticancer chemical drug solution, stirred or intermittently pulsed and ultrasonically reacted for 0.5-24 hours, the reaction product is adsorbed by a magnet, and the unreacted reactant is removed. After washing several times with pure water or phosphate buffer solution, the magnetosomes are sucked out with a magnet to obtain the product.

[0091] Experimental results:

[0092] Drug loading method

coupling agent

Concentration of coupling agent

C BMP

(mg / ml)

C ADM

(mg / ml)

C ADM / C BMP

Drug loading

(μgADM / mgBMP)

direct coupling one-step

direct coupling one-step

direct coupling one-step

direct coupling one-step

direct coupling one-step

direct coupling...

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Abstract

The invention relates to a biological nanometer magnetic target anti-cancer drug, which is formed by coupled biological nanometer magnetic smalls and anti-cancer chemical drug. Wherein, the biological nanometer magnetic small is nanometer magnetic particle formed in magnetic bacterial cell, at 35-120nm diameter, while its main component is Fe3O4 or Fe3S4; single magnetic particle is packed by film; and the coupled anti-cancer chemical drug comprises chemotherapy drug, nucleic acid drug, radioactive nuclide or antibody drug. The invention also provides relative production that using physical adsorption couple or based on the active function group contained by smalls and anti-cancer drug, to select right coupler via chemical reaction to couple them. And the smalls has better bioavailability, less side effect, therefore, the anti-cancer drug can be accurately transmitted to ill part via external magnetic field, to reduce the contact between drug and normal organism., to realize target positioning treatment.

Description

technical field [0001] The invention relates to a bionano magnetic targeting anticancer drug, which is a magnetic targeting drug using a bionano magnetosome as a carrier. Background technique [0002] At present, cancer is the chronic disease with the highest mortality rate in human beings. So far, most of the cancer treatment still adopts traditional systemic chemotherapy. Because the chemical drugs are distributed in the patient's body, they will cause toxicity to the patient's normal tissue cells, and there are many side effects in clinical use, such as Hair loss, spinal cord toxicity, liver and kidney failure, etc., cause adverse reactions, causing cancer patients to suffer unbearably. Many patients eventually die not from the cancer itself, but from the side effects caused by chemotherapy. [0003] In order to improve the effect of tumor chemotherapy and reduce the side effects of drugs on normal tissues, in recent decades, various new drug delivery systems for local ta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/48A61K45/06A61K51/06A61P35/00H01F1/11A61K47/52
Inventor 唐喜庆
Owner 南京英睿杰生化科技有限公司
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