Facultative anaerobic magnetotactic bacterium, and separation and culturing method therefor, and process for extracting and purifying magnetosome thereof

Abstract

The invention is about a kind of magnetotactic bacterium which is separated from the mud sample in the iron-affluent watershed. The bacterium is preserved in the Chinese typical culture collection center. The preserving code is CCTCC NO:M205105 while the classified name is Magnetotactic bacterium MB-TZ. The bacterium is facultative anoxybiontic bacterium and the state of the thallus is circular staff. A thallus can produce 3-20 circular magnetic globules which are mainly comprised by iron, calcium, fosforus, oxygen and magnesium. The bacterium has many advantages such as short producing cycle, easy to cultivate and easy to be produced in factories. During the extracting and purifying of the magnetic globules, the invention adopts the treating method of repeating freeze thawing combining with supersonics, so it can dissolve the thallus more thoroughly and increase the yield of magnetic globules.

Description

technical field [0001] The invention relates to a magnetotactic bacterium (Magnetotactic bacterium, MB for short) capable of producing magnetosomes, a method for isolating and purifying the magnetotactic bacterium, and a method for extracting and purifying the magnetotactic bacterium from the magnetotactic bacterium. [0002] The magnetotactic bacterium MB-TZ is isolated from muddy water samples extracted from waters rich in iron ore. The magnetotactic bacterium MB-TZ is facultatively anaerobic, easy to cultivate, and has a short growth period. It has been preserved in the China Type Culture Collection Center, address: Wuhan University, Wuhan, China, preservation date: September 20, 2005, preservation number: CCTCC NO: M 205105, and classification name: Magnetotactic bacterium MB-TZ. Background technique [0003] Magnetotactic bacteria are a type of bacteria that can form nanoscale magnetic particles called magnetosomes in the bacteria, and can move towards one end of the ma...

AI Technical Summary

Problems solved by technology

Chinese patents ZL02115867.3 and ZL02115868.1 respectively proposed "a kind of sludge magnetotactic bacteria and its method of separation and purification and preparation of magnetosomes" and "a kind of iron ore magnetotactic bacteria and its method of separation and purification and preparation of magnetosomes". method", although the magnetosomes of biological origin can be obtained, but because the magnetotactic bacteria used are anaerobic bacteria, they must be separated and purified in a sealed state

Therefore, there are disadvantages of high process requirements and complicated production equipment, especially the process of preparing magnetosomes takes 45--85 days, and there is a shortcoming of long production cycle and difficulty in realizing industrial production