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Method for rapid detection of bacteria

A bacterial and rapid technology, applied in biochemical equipment and methods, microbiological determination/inspection, etc., can solve the problems of affecting the detection effect, inability to distinguish between dead and living bacteria, expensive equipment, etc.

Inactive Publication Date: 2007-05-30
阮信畅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Existing bacterial detection technology requires expensive equipment and a long detection time. At present, the most commonly used detection technology is to separate and cultivate bacteria, and observe with a microscope after forming a single bacterial colony
This process takes more than one day, and it is easy to be contaminated during the cultivation and isolation of bacteria, which will affect the detection effect;
[0003] Another commonly used detection technique is the use of a gene cycler (PCR), which is relatively fast, but generally takes more than a few hours, and this detection method cannot distinguish between dead and live bacteria

Method used

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  • Method for rapid detection of bacteria
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Embodiment Construction

[0022] Referring to FIGS. 1-5 , in the embodiment of the present invention, the detection antenna can be formed by plating, evaporating, depositing two metal films 9 on the chip substrate 8 , and each of the two metal films 9 protrudes from a tip. The metal film 9 other than the tip is separated from the outside world by the protective layer 10, and only the two tips can contact the added liquid or gas. The metal thin film 9 is electrically interconnected with the amplifier 4 through wires.

[0023] The metal film is chemically stable and corrosion-resistant, such as titanium, chromium, gold, platinum, etc. It is also possible to use a single piece or multiple pieces, but two pieces are preferred.

[0024] When detecting bacteria, the solution containing the bacteria 1 to be tested is mixed with the solution containing the known bacteriophage 2 and dropped on the two tips of the metal film 9, the voltage between the two metal films 9 is amplified by the amplifier 4 or sent in...

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Abstract

The invention discloses a rapid bacteria detecting method, which is characterized by the following: blending bacterial sample with phage solution; amplifying pressure noise of mixed solution to analyze; comparing noise character and reference character to obtain corresponding bacteria; blending the detected bacterial sample and mixed bacterial solution on the antenna; detecting whether the bacteria are infected; affirming the bacteria within 8-60min.

Description

technical field [0001] The invention belongs to the field of biological detection and relates to a technology for rapid detection of bacteria. Background technique [0002] Existing bacterial detection technology requires expensive equipment and a long detection time. Currently, the most commonly used detection technology is to separate and cultivate bacteria, and then observe with a microscope after forming a single bacterial colony. This process takes more than one day, and it is easy to be contaminated during the cultivation and isolation of bacteria, which will affect the detection effect; [0003] Another commonly used detection technique is the use of a gene cycler (PCR), which is relatively fast, but generally takes more than a few hours, and this detection method cannot distinguish between dead and live bacteria. Contents of the invention [0004] The purpose of the present invention is to provide a new bacteria detection technology, which can detect and identify ...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
Inventor 阮信畅
Owner 阮信畅