Preparative separation/purification system

Abstract

A mixed liquid passage (31) is connected to a trapping passage (22) at a point upstream of a trap column (23). A mixture of a diluting liquid and an additive agent, which are respectively drawn and sent by pumps (35) and (36), is supplied into the mixed liquid passage (31). When an eluate containing a target compound is passed through the trap column (23), the eluate is diluted with the diluting liquid and hence its elution capability decreases, so that the target compound can be easily captured in the trap column (23). Meanwhile, the solubility of the mobile phase in the eluate decreases, causing not only the target component but also foreign compounds to more easily precipitate. However, their precipitation is prevented by the effect of the additive agent. Thus, clogging of the trap column (23) or pipes is prevented.

Description

TECHNICAL FIELD[0001]The present invention relates to a preparative separation / purification system for separately purifying and collecting one or more components individually extracted from a solution by a liquid chromatograph. More specifically, it relates to a preparative separation / purification system using a trap column for temporarily capturing a target component.BACKGROUND ART[0002]For example, in the pharmaceutical industry, preparative separation / purification systems utilizing liquid chromatographs are used to collect samples of a variety of chemically synthesized compounds in order to store those samples in a library or analyze them in more detail. Conventional examples of the preparative separation / purification system are disclosed in Patent Documents 1 and 2.[0003]In these conventional apparatuses, target components (compounds) in a sample solution are temporally separated by a liquid chromatograph. The separated target components are then respectively introduced into dif...

AI Technical Summary

Benefits of technology

[0009]The present invention has been developed in view of the previously described problems, and its primary target is to provide a preparative separation / purification system capable of assuredly capturing a target compound with high levels of efficiency while preventing clogging or other problems of the trap column or pipes.Means for Solving the Problems

Problems solved by technology

However, lowering the elution capability of the mobile phase in this manner decreases not only the solubility of the target compound in the mobile phase but also that of foreign compounds.

As a result, the target and foreign compounds both easily precipitate within a pipe or trap column, causing clogging or similar problems and thereby impeding the flow of the solutions.

Furthermore, even if a diluting liquid, such as water, is mixed into the mobile phase, the adsorbent in the trap column cannot exhibit an adequate retaining power for some kinds of compounds but allows those compounds to pass through the trap column.

This not only results in a failure of the preparative separation but may also cause a loss of the synthesized pharmacological compound.

However, in the pharmaceutical industry, the preparative separation / purification needs to be performed on such a wide variety of compounds that it requires an extreme amount of time, labor and cost to determine optimal conditions for each and every compound.

However, none of the conventional systems could meet with this requirement.

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