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Reagents and methods and systems using them

a technology of heavy metals and reagents, applied in the field of reagents and systems for use in testing heavy metals, can solve the problem of remaining difficult to accurately measure the lead levels of various individuals

Inactive Publication Date: 2012-10-04
MAGELLAN DIAGNOSTICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent describes a composition and method for measuring heavy metals in mammalian fluid samples. The composition includes a lysing agent to lyse cells and an adsorber to remove interfering sulfhydryl species. The adsorber can be a powdered, activated carbon. The method involves adding the sample to a container with the lysing agent and adsorber, and measuring the level of heavy metal using a sensor such as colloidal gold or a lead detector. The composition and method can be used in various applications such as medical diagnostics and environmental monitoring.

Problems solved by technology

It remains difficult to accurately measure the lead levels in these various individuals.

Method used

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  • Reagents and methods and systems using them
  • Reagents and methods and systems using them
  • Reagents and methods and systems using them

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0077]To determine the effect of various adsorbents on GSH removal, four different adsorbent were tested. These included three adsorbents available from Norit Americas Inc—E Supra (surface area of 900 m2 / gram, density of 360 kg / m3 and particle size range from 5-100 microns), SX Ultra (surface area of 1,200 m2 / gram, density of 320 kg / m3 and particle size range from 5-100 microns), and Darco® S-51HF (surface area of 650 m2 / gram, density of 510 kg / m3 and particle size range from 5-100 microns). A fourth activated carbon (Filtracarb SXO, which included a surface area of 1,200 m2 / gram, a density of 480 kg / m3 and a particle size range from 45-200 microns) was also used.

[0078]Various amounts of the adsorbents were mixed with 1.7 mM GSH and 40 micrograms / deciliter Pb. the lead levels were analyzed using LeadCare strips and the LeadCare assay protocol. The results as a function of concentration of adsorbent are shown in FIG. 3. Each of the various adsorbent were effective to remove the GSH, ...

example 2

[0079]To ascertain if the concentration of the adsorbent material affected the signal response of the assay, high concentrations of the adsorbents used in Example 1 were added to the mixture and the lead levels were again assayed. The results are shown in FIG. 4. As adsorbent concentration exceeded 1 mg / mL, the signal decreased linearly.

example 3

[0080]To determine if the adsorbent was adsorbing a substantial amount of the lead in a sample, the impact of the adsorbent in the absence of GSH was measured using 40 micrograms / deciliter of Pb. The LeadCare II assay (FIG. 5) and a graphite atomic absorption furnace analysis (FIG. 6) were performed using adsorbents E Supra and SXU, respectively. Both adsorbents were observed to remove a small amount of lead in the absence of GSH.

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Abstract

Certain embodiments described herein are directed to a reagent that includes an effective amount of an adsorber to remove interfering species present during heavy metal level measurement in a fluid sample. In some examples, the reagent can include an effective amount of an adsorber to remove a suitable amount of glutathione from the fluid sample such that the glutathione does not interfere with measurements of lead levels in the fluid sample.

Description

TECHNOLOGICAL FIELD[0001]This is application is related to reagents and systems for use in testing heavy metals. More particularly, certain embodiments herein are directed to reagents including an effective amount of an adsorber to remove species that may interfere with lead level measurements.BACKGROUND[0002]Lead levels are commonly measured in children and infants. Lead levels are also routinely measured in certain professions where manufacturing may expose the workers to lead. It remains difficult to accurately measure the lead levels in these various individuals.SUMMARY[0003]In a first aspect, a composition comprising an effective amount of a lysing agent to lyse cells in a mammalian fluid sample is provided. In some examples, the composition can also include an effective amount of an adsorber to adsorb substantially all glutathione present in the mammalian fluid sample to be added to the composition.[0004]In certain embodiments, the effective amount of the lysing agent is enoug...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): G01N33/20
CPCG01N33/84Y10S435/81Y10T436/108331Y10T436/107497Y10T436/23
Inventor SCOTT, W. JAMES
Owner MAGELLAN DIAGNOSTICS