Light sheet generator
a technology of light sheets and generators, applied in the field of microscope sample illumination, can solve the problems of cumbersome agarose embouchure of samples
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example 2
[0050]In some embodiments, a diSPIM microscope (e.g., device 44) may utilize two light sheet scanners, such as light sheet generators 10, 100, and / or 100′, one for each objective. Each light sheet scanner may be used in an arm portion of the microscope. The scanners may use integrated 2D MEMS mirrors to provide light weight, low vibration optical scanning of light from a single mode fiber-coupled laser. The basic design of the unit may include a series of 4f lens pair arrangements with the scan mirrors, apertures, and focal planes located at the foci of the lenses, as described above.
[0051]The input to each light sheet scanner may include a fiber collimator (e.g., collimator 114, 114′) that accepts a single-mode FC / PC (or optionally FC / APC) connected fiber-coupled laser source (e.g., source 112, 112′). The fiber collimator may be an exchangeable part that allows some flexibility in the focal length, and hence intrinsic beam diameter, and fiber connector type. The collimated laser be...
example 3
[0055]This example describes a method for generating a light sheet suitable for use in a SPIM microscope; see FIG. 4. Aspects of the light sheet generators described above may be utilized in the method steps described below. Where appropriate, reference may be made to previously described components and systems that may be used in carrying out each step. These references are for illustration, and are not intended to limit the possible ways of carrying out any particular step of the method.
[0056]FIG. 4 is a flowchart illustrating steps performed in an illustrative method, and may not recite the complete process or all steps of the method. FIG. 4 depicts multiple steps of a method, generally indicated at 200, which may be performed in conjunction with a light sheet generator according to aspects of the present disclosure. Although various steps of method 200 are described below and depicted in FIG. 4, the steps need not necessarily all be performed, and in some cases may be performed ...
example 4
[0065]This section describes additional aspects and features of compact light sheet generators, presented without limitation as a series of paragraphs, some or all of which may be alphanumerically designated for clarity and efficiency. Each of these paragraphs can be combined with one or more other paragraphs, and / or with disclosure from elsewhere in this application, including the materials incorporated by reference in the Cross-References, in any suitable manner. Some of the paragraphs below expressly refer to and further limit other paragraphs, providing without limitation examples of some of the suitable combinations.
[0066]A0. A system for generating a light sheet for use in a microscope, such as a SPIM, iSPIM, and / or diSPIM microscope, the system comprising:
[0067]a housing including a plurality of elongate internal chambers in sequential communication with each other and containing an optics system, such that a first chamber contains a first lens, a second chamber extends from ...
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