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In vitro prediction of in vivo half-life

Inactive Publication Date: 2017-08-10
F HOFFMANN LA ROCHE & CO AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent text is about a method to increase the lifespan of an antibody in the body. This is done by changing certain charged amino acid residues in the antibody to hydrophobic or neutral hydrophilic residues. This change affects the way the antibody interacts with a protein called FcRn, which prevents the antibody from being broken down in the body. The result is a longer half-life for the antibody.

Problems solved by technology

However, the underlying mechanism how the Fab region influences FcRn binding is still not elucidated.

Method used

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  • In vitro prediction of in vivo half-life
  • In vitro prediction of in vivo half-life
  • In vitro prediction of in vivo half-life

Examples

Experimental program
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specific embodiments

[0414]1. A method for selecting an antibody comprising the following steps:[0415]i) determining a first retention time of the antibody and a reference antibody on an FcRn affinity chromatography column with a positive linear pH gradient elution in the presence of a first salt concentration, and determining a second retention time of the antibody and the reference antibody on an FcRn affinity chromatography column with the positive linear pH gradient elution in the presence of a second salt concentration, or[0416]ii) determining a first retention time of the antibody and a reference antibody on an FcRn affinity chromatography column with a linear salt gradient elution at a first pH value, and determining a second retention time of the antibody and the reference antibody on an FcRn affinity chromatography column with the linear salt gradient elution at a second pH value, or[0417]iii) determining for the antibody and a reference antibody the KD value at pH 6 using surface plasmon reson...

example 1

[0669]Preparation of FcRn Affinity Column

[0670]Expression of FcRn in HEK293 Cells

[0671]FcRn was transiently expressed by transfection of HEK293 cells with two plasmids containing the coding sequence of FcRn and of beta-2-microglobulin. The transfected cells were cultured in shaker flasks at 36.5° C., 120 rpm (shaker amplitude 5 cm), 80% humidity and 7% CO2. The cells were diluted every 2-3 days to a density of 3 to 4*105 cells / ml.

[0672]For transient expression, a 14 l stainless steel bioreactor was started with a culture volume of 8 l at 36.5° C., pH 7.0±0.2, pO2 35% (gassing with N2 and air, total gas flow 200 ml min−1) and a stirrer speed of 100-400 rpm. When the cell density reached 20*105 cells / ml, 10 mg plasmid DNA (equimolar amounts of both plasmids) was diluted in 400 ml Opti-MEM (Invitrogen). 20 ml of 293fectin (Invitrogen) was added to this mixture, which was then incubated for 15 minutes at room temperature and subsequently transferred into the fermenter. From the next day...

example 2

[0678]Chromatography Using the FcRn Affinity Column and pH Gradient

[0679]The receptor derivatized sepharose was filled in a 1 ml XK column (GE Healthcare) and the FcRn column then was equilibrated with 20 mM 2-(N-morpholine)-ethanesulfonic acid (MES) buffer containing 140 mM NaCl, pH 5.5.

[0680]Conditions:[0681]column dimensions: 50 mm×5 mm[0682]bed height: 5 cm[0683]loading: 30 μg sample[0684]equilibration buffer: 20 mM IViES, with 140 mM NaCl, adjusted to pH 5.5[0685]elution buffer: 20 mM Tris / HCl, with 140 mM NaCl, adjusted to pH 8.8[0686]elution: 7.5 CV equilibration buffer, in 120 min. to 100% elution buffer, 10 CV elution buffer

[0687]The samples were prepared in 20 mM 2-(N-morpholine)-ethanesulfonic acid (MES) sodium salt, 140 mM sodium chloride, pH 5.5. Each sample contained 30 μg mAb per injection. Antibodies were eluted by a linear pH gradient from pH 5.5 to 8.8 within 120 minutes using 20 mM 2-(N-morpholine)-ethanesulfonic acid (MES) sodium salt, 140 mM sodium chloride, pH ...

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Abstract

Herein is reported a method for determining the presence of antibody-Fab-FcRn interaction in an antibody-Fc-FcRn complex influencing the in vivo half-life comprising the steps of a) determining the retention time of the antibody on an FcRn affinity chromatography column with a positive linear pH gradient elution in the presence of a first sodium chloride concentration, and b) determining the retention time of the antibody on an FcRn affinity chromatography column with a positive linear pH gradient elution in the presence of a second sodium chloride concentration, whereby the presence of antibody-Fab-FcRn interaction in an antibody-Fc-FcRn complex influencing the in vivo half-life is determined if the retention time determined in step a) and the retention time determined in step b) are substantially different.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application is a continuation of International Application No. PCT / EP2015 / 055482 having an international filing date of Mar. 17, 2015, the entire contents of which are incorporated herein by reference, and which claims benefit under 35 U.S.C. §119 to European Patent Application No. 14161103.8 filed on Mar. 21, 2014 and European Patent Application No. 14165987.0 filed on Apr. 25, 2014.SEQUENCE LISTING[0002]The instant application contains a Sequence Listing submitted via EFS-Web and hereby incorporated by reference in its entirety. Said ASCII copy, created on Sep. 20, 2016, is named P32047US_SeqList.txt, and is 93,656 bytes in size.FIELD OF THE INVENTION[0003]The current invention is in the field of recombinant antibody technology, especially in the field of tailor made antibodies. Herein is reported a method for the prediction of the in vivo half-life of an antibody based on the retention time determined on an FcRn affinity chromatog...

Claims

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Application Information

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IPC IPC(8): G01N33/68B01D15/38B01D15/16
CPCG01N33/6854B01D15/3809B01D15/168C07K16/00C07K2317/94C07K2317/52
Inventor EMRICH, THOMASKETTENBERGER, HUBERTSCHLOTHAUER, TILMANSCHOCH, ANGELA
Owner F HOFFMANN LA ROCHE & CO AG
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