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High purity alpha yohimbine (rauwolscine) from rauwolfia species

a technology of alpha yohimbine and rauwolfia, which is applied in the direction of solvent extraction, plant/algae/fungi/lichens ingredients, separation processes, etc., can solve the problems of unviable extraction and purification of compounds at commercial scale, enhancing extraction cost, and reducing yield of column chromatography. achieve the effect of better source and higher yield

Pending Publication Date: 2020-12-10
GOEL PAWAN KUMAR
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The invention discuses using a renewable source, namely the leaves of a specific species of Rauwolfia, as a raw material instead of bark. This is because the leaves contain a higher percentage of the active compound, resulting in a higher yield.

Problems solved by technology

The methodology is complex involving multiple fractionation techniques involving use of organic solvents, acidification and basification and also use of column chromatography (flash chromatography) to fracionate alpha yohimbine from other compounds The method is more of a laboratory scale method for isolation and purification of alkaloid compounds from Rauwolfia sp. for research purposes, than a process for commercial scale production of alpha yohimbine.
Prior art methods have used column chromatography and expensive matrices to purify alpha-yohimbine.
Column chromatography reduces yields and enhances the cost of extraction, making it unviable for extraction and purification of compounds at commercial scale, though it is very much suitable for laboratory scale preparation.

Method used

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  • High purity alpha yohimbine (rauwolscine) from rauwolfia species
  • High purity alpha yohimbine (rauwolscine) from rauwolfia species
  • High purity alpha yohimbine (rauwolscine) from rauwolfia species

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[0075]Collection of raw material and powdering: 1000 g leaves of Rauwolfia canescens were collected, dried in the sun and powdered. Extraction was then carried as described in the steps below:[0076]1. Organic solvant—Alkaline Extraction Step: The powder was then mixed with water immiscible solvent viz. any non polar organic solvent such as toluene etc. preferably toluene at 40-50° C. in ratio of 1:4 i.e. 1 part solid and 4 parts solvent and made alkaline by addition of 20-25% ammonia solution, pH 9-9.5 (alkaline) and kept for 4 hours in reactor with stirring followed by filtration. The filtrates / extracts were then transferred to another vessel. The remaining solid material was extracted twice with same solvent as above and the filtrates were pooled in the same vessel.[0077]2. Organic solvent-Acidic extraction step: The pooled fractions were then made acidic by addition of tartaric acid solution (pH 3.5±0.5) and extracted with purified water in optimal ratio of 12:1 v / v (volume of po...

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Abstract

Present invention discloses a novel, commercially viable process for extraction of Alpha yohimbine from the bark, stem and leaves of Rauwolfia species. The extract is obtained by a precipitation method involving alternate steps of acidification and alkalization along with use of specific organic solvents. The extract gives higher yield of 7-8 fold as compared to other processes and without use of any column chromatography at all. Yield of alpha yohimbine from roots and leaves of Rauwolfia canescens by process of present invention was 0.017% and 0.4% respectively, indicating that leaves are a much better source. The HPLC analysis of the compound obtained indicated a purity of >90%.

Description

[0001]The present invention relates to natural extracts / herbal extracts More specifically, it pertains to a novel process for the extraction of alpha yohimbine (rauwolscine) from Rauwolfia species. The process gives alpha yohimbine in significantly higher quantity and purity as compared to prior art methods.BACKGROUND OF THE INVENTION[0002]Terms and Definitions[0003]Alkaloids—These are a group of naturally occurring chemical compounds that mostly contain basic nitrogen atoms (https: / / en.wikipedia.org / wiki / Alkaloid). They are of plant origin and have pronounced physiological actions on humans. They include many drugs (morphine, quinine) and poisons (atropine, strychnine).[0004]Phytochemicals—These are compounds derived from plants.[0005]Organic solvents—Organic solvents are a chemical class of compounds that share a common structure (at least 1 carbon atom and 1 hydrogen atom), low molecular weight, lipophilicity, and volatility, and they exist in liquid form at room temperature. The...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C07D459/00B01D11/02
CPCB01D11/0288C07D459/00A61K36/24
Inventor TEWARI, KIRANSHARMA, ASHOK
Owner GOEL PAWAN KUMAR
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