Method for repairing living tissue with a hollow fiber scaffold
a hollow fiber and living tissue technology, applied in the field of tissue engineering, electrospinning, and biocompatible scaffolding, can solve problems such as the development of hollow fibrous structures, and achieve the effects of enhancing healing or regeneration, and facilitating healing or regeneration of jaws or teeth
Active Publication Date: 2021-10-14
KING ABDULAZIZ UNIV
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Benefits of technology
[0028]In some embodiments, the scaffold may be preseeded with stem cells or cells similar or identical to the tissue or organ in need of regeneration. In other embodiments a scaffold once in place may become populated with cells that migrate into it or otherwise attach to it and help heal or regenerate a target tissue.
[0031]In another embodiment, the scaffold as disclosed herein may be used to treat or enhance healing or regeneration of a poorly vascularized or avascular tissues such dense connective tissue or cartilage.
Problems solved by technology
Despite the flexibility and other advantages of electrospinning processes, few studies report the development of a hollow fibrous structure by electrospinning due to the complexity in preparing and characterizing hollow electrospun fiber structures; Lee, G. H., et al., Controlled wall thickness and porosity of polymeric hollow nanofibers by coaxial electrospinning.
This is a significant problem because hollow fibrous scaffolds are highly desirable in many tissue engineering applications.
Method used
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[0085]Materials. Polylactic acid (PLA 2003D) was purchased from Nature Works, USA. Poly butylene succinate (PBS, commercial name is Bionolle) was obtained from Showa Denko, Japan. Cellulose Nanofibrils (CNF) was acquired from University of Maine, USA. Dulbecco's Modified Eagle's Medium (DMEM) was purchased from Lonza, USA. Proteinase
[0086]K solution (20 mg / m1), Dulbecco's phosphate-buffered saline and 10% fetal bovine serum (FBS) was purchased from Thermo Fisher Scientific Inc., Fair Lawn, N.J. Glycerol was obtained from Loba Chemie Pvt Ltd, India. Mineral oil (P3) is from Pfeiffer Vacuum, Germany. Chloroform, ethanol, sodium dodecyl sulfate (SDS), Lysozyme, tris buffer solution (0.1 M, pH-7), calcium chloride (CaCl2) and sodium azide (NaN3) were from Sigma-Aldrich, St. Louis, Mo., USA.
[0087]Preparation of hollow fibrous scaffold. A mixture of PLA and PBS at a weight ratio of 50 / 50 was dissolved in chloroform and ethanol with 3:1 volume ratio by stirring for 3 hours.
[0088]In order t...
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Abstract
A scaffold of hollow fibers comprising a mixture of polylactic acid (PLA) and polybutylene succinate (PBS) and cellulose nanofibers (CNF), medical products made of these scaffolds and methods of using the scaffolds in regenerative medicine. A method for producing the scaffolds is also disclosed.
Description
STATEMENT REGARDING PRIOR DISCLOSURES BY THE INVENTOR OR A JOINT INVENTOR[0001]Aspects of this technology are described by Abudula, T., Saeed, U., Memic, A. et al. in Electrospun cellulose Nano fibril reinforced PLA / PBS composite scaffold for vascular tissue engineering. J Polym Res26, 110 (Apr. 15, 2019) doi:10.1007 / 510965-019-1772-y which is incorporated herein by reference in its entirety.BACKGROUND OF THE INVENTIONField of the Invention[0002]The invention pertains to the fields of tissue engineering, electrospinning, and biocompatible scaffolding.Description of Related Art[0003]The design and production of scaffolds for tissue engineering by electrospinning is a topic of great interest. This technology can produce scaffolds composed of micro and nano fibers from a wide variety of synthetic and natural polymers which can mimic many properties of materials found in human cells; Kishan, A. P., et al., Recent advancements in electrospinning design for tissue engineering applications...
Claims
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IPC IPC(8): A61L27/26C08L67/04D01D5/00C08L1/02C08L67/02
CPCA61L27/26C08L67/04D01D5/003D10B2201/28C08L67/02D10B2331/041C08L1/02A61L27/56A61L2400/12D01F1/02D01F1/08D01F1/103D01F6/625D01F8/02
Inventor ABUDULA, TUERDIMAIMAITISAEED, USMANAL-TURAIF, HAMADALSHAHRIE, AHMED
Owner KING ABDULAZIZ UNIV