The present invention discloses a method for preparing nanometer
liposome by using a
supergravity technology. The method comprises: mixing an organic solution containing a fat material and an
aqueous solution containing a carrier by using a
supergravity rotation
bed, and carrying out
freeze drying treatment to obtain the nanometer
liposome. According to the present invention, by using the excellent
mass transfer ability of the
supergravity rotation filling
bed, the preparation process of the nanometer
liposome is performed under the highly-microscopic and uniform condition so as to obtain the nanometer liposome particles with characteristics of controlled particle size, narrow distribution and high stability; by adding the freeze-
drying protection agent, the breaking aggregation of the nanometer liposome during the freeze-
drying process can be avoided, and the process for forming the nanometer liposome through the hydration of the freeze-dried product can be accelerated; the
organic solvent is treated by using the freeze-
drying method, such that the operation is simple, and the
organic solvent removing effect is good; the method has advantages of simple process, less
energy consumption, high efficiency, low cost, and easy large-scale industrial production; and the average particle size of the obtained nanometer liposomes is 20-200 nm, the PDI is 0.1-0.3, and the method is suitable for large-scale production.