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DNA print thermo-sensitive high molecule material and preparation process thereof

A polymer material and imprinting technology, which is applied in the analysis of materials, material separation, instruments, etc., can solve the problems of small polymer surface area, recognition and separation of macromolecules, etc.

Inactive Publication Date: 2007-11-14
TIANJIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although protein imprinting on the polymer surface can make the protein eluted smoothly from the polymer surface (see Shi, H.; Tsai, W.B.; Garrison, M.D.; Ferrari, S.; & Ratner, B.D. Template-imprinted nanostructured surfaces for protein recognition. Nature, 1999, 398:593-597), but the polymer surface area is too small to effectively recognize and separate macromolecules

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: A DNA blotting thermosensitive polymer material, characterized in that: the polymer material has a monomer unit structure of N-isopropylacrylamide; the polymer material is a cross-linked polymer; the polymer The structure of the material contains holes imprinted by DNA.

[0019] In the above-mentioned Southern blotting thermosensitive polymer material, the volume of the polymer in the aqueous solution will change with the change of temperature.

[0020] For the aforementioned thermally sensitive polymer material for Southern blotting, the polymer is insoluble in any solvent.

[0021] A method for preparing Southern blotting thermosensitive polymer material, which is characterized in that it consists of the following steps:

[0022] 1. Preparation of polymer:

[0023] Add 5g of N-isopropylacrylamide and 2g of N,N'-methylenebisacrylamide to 10mL of 50μg / mL salmon test DNA aqueous solution; incubate the solution at 5℃ for 1h for use before polymerization DNA mole...

Embodiment 2

[0030] Embodiment 2: A Southern blotting thermosensitive polymer material, characterized in that: the polymer material has a monomer unit structure of N-isopropylacrylamide; the polymer material is a cross-linked polymer; the polymer The structure of the material contains holes imprinted by DNA.

[0031] In the above-mentioned Southern blotting thermosensitive polymer material, the volume of the polymer in the aqueous solution will change with the change of temperature.

[0032] For the aforementioned thermally sensitive polymer material for Southern blotting, the polymer is insoluble in any solvent.

[0033] A method for preparing Southern blotting thermosensitive polymer material, which is characterized in that it consists of the following steps:

[0034] 1. Preparation of polymer:

[0035] Add 10g of N-isopropylacrylamide and 4g of N,N'-methylenebisacrylamide to 20mL of 50μg / mL salmon test DNA aqueous solution; incubate the solution for 2h at 5℃ for use before polymerization DN...

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PUM

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Abstract

The invention relates to a polymer that could take identification to the red fish testis DNA molecule. It includes the following steps: embedding the plate DNA into poly N-isopropyl acrylamide, using the feature of the hydrophilous nature of poly N-isopropyl acrylamide would change following the altering of temperature, the polymer would expand and contract in water following the temperature to promote the plate macromolecule washing out form polymer mother body to gain polymer of DNA print inanition. The polymer could identify the red fish testis DNA.

Description

(1) Technical field: [0001] The present invention belongs to the synthetic field of DNA imprinting polymer in biological macromolecular imprinting technology, in particular to a DNA imprinting thermosensitive polymer material and a preparation method thereof. The technology is developed on the basis of small molecular imprinting technology and is used for preparation New materials that identify specific DNA molecules and provide a way to solve the problem of template molecules coming out of macromolecular imprinting. (2) Background technology: [0002] Southern blotting polymer is a material that can recognize specific DNA macromolecules. Its development as a biological macromolecular imprinting technology originated from small molecular imprinting technology (MIT) (see Wulff, G. Molecular imprinting in cross-linked materials with the aid of molecular template a way towards artificial antibodies. Angew. Chem. Int. Ed. , 1995, 34: 1821-1832.). MIT has been widely used in HPLC stat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F220/56C08F2/44C08F6/00G01N33/566G01N30/00
Inventor 黄积涛郑嗣华张嘉琪黄卫洪谢秀荣
Owner TIANJIN UNIVERSITY OF TECHNOLOGY