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DNA print thermo-sensitive high molecule material and preparation process thereof

A polymer material and imprinting technology, which is applied in the analysis of materials, material separation, instruments, etc., can solve the problems of small polymer surface area, recognition and separation of macromolecules, etc.

Inactive Publication Date: 2006-06-28
TIANJIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although protein imprinting on the surface of polymers allows for smooth elution of proteins from polymer surfaces (see Shi, H.; Tsai, W.B.; Garrison, M.D.; Ferrari, S.; & Ratner, B.D. Template-imprinted nanostmctured surfaces for protein recognition. Nature , 1999, 398:593-597), but the polymer surface area is too small to effectively recognize and separate macromolecules

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: A kind of Southern imprint thermosensitive polymer material, it is characterized in that: the polymer material has the monomer unit structure of N-isopropylacrylamide; The polymer material is a cross-linked polymer; The polymer material The material structure contains cavities where the DNA is imprinted.

[0019] For the aforementioned Southern imprinted thermosensitive polymer material, the volume of the polymer in the aqueous solution will change as the temperature changes.

[0020] In the aforementioned Southern imprint thermosensitive polymer material, the polymer thereof is insoluble in any solvent.

[0021] A method for preparing a Southern imprinted thermosensitive polymer material is characterized in that it consists of the following steps:

[0022] 1. Preparation of polymer:

[0023] Add 5 g of N-isopropylacrylamide and 2 g of N,N′-methylenebisacrylamide to 10 mL of 50 μg / mL aqueous solution of salmon testis DNA; DNA molecules orient the monome...

Embodiment 2

[0030] Embodiment 2: A kind of Southern imprint thermosensitive polymer material, it is characterized in that: the polymer material has the monomer unit structure of N-isopropylacrylamide; The polymer material is a cross-linked polymer; The polymer material The material structure contains cavities where the DNA is imprinted.

[0031] For the aforementioned Southern imprinted thermosensitive polymer material, the volume of the polymer in the aqueous solution will change as the temperature changes.

[0032] In the above-mentioned Southern imprinting thermosensitive polymer material, the polymer thereof is insoluble in any solvent.

[0033] A method for preparing a Southern imprinted thermosensitive polymer material is characterized in that it consists of the following steps:

[0034] 1. Preparation of polymer:

[0035] Add 10 g of N-isopropylacrylamide and 4 g of N,N'-methylenebisacrylamide to 20 mL of 50 μg / mL aqueous solution of salmon testis DNA; Orient the molecules to th...

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PUM

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Abstract

The invention relates to a polymer that could take identification to the red fish testis DNA molecule. It includes the following steps: embedding the plate DNA into poly N-isopropyl acrylamide, using the feature of the hydrophilous nature of poly N-isopropyl acrylamide would change following the altering of temperature, the polymer would expand and contract in water following the temperature to promote the plate macromolecule washing out form polymer mother body to gain polymer of DNA print inanition. The polymer could identify the red fish testis DNA.

Description

(1) Technical field: [0001] The invention belongs to the field of synthesis of DNA imprinted polymers in biomacromolecule imprinting technology, in particular to a DNA imprinted thermosensitive polymer material and its preparation method. This technology is developed on the basis of small molecule imprinting technology and is used for the preparation of New materials that recognize specific DNA molecules and provide a way to address the problem of template molecule dropout in macromolecular imprinting. (two) background technology: [0002] Southern imprinted polymers are materials that recognize specific DNA macromolecules. Its development as biomacromolecule imprinting technology comes from small molecule imprinting technology (MIT) (see Wulff, G. Molecular imprinting in cross-linked materials with the aid of molecular template a way towards artificial antibodies. Angew. Chem. Int. Ed. , 1995, 34: 1821-1832.). MIT has been widely used in the fields of HPLC stationary phas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F220/56C08F2/44C08F6/00G01N30/00G01N33/566
Inventor 黄积涛郑嗣华张嘉琪黄卫洪谢秀荣
Owner TIANJIN UNIVERSITY OF TECHNOLOGY