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RNA blot electro-response hydrogel and its preparing method

A technology of RNA imprinting and hydrogel, applied in the field of ribonucleic acid imprinting electro-responsive hydrogel and its preparation, can solve the problems of identification and separation of macromolecules, small surface area of ​​polymers, etc., and achieve the effect of promoting prolapse

Inactive Publication Date: 2008-02-27
TIANJIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, biomacromolecular templates such as RNA are difficult to detach from the polymer because the size of the macromolecule is much larger than the polymer mesh
Although biomacromolecules are imprinted on the polymer surface, the macromolecular template can be eluted smoothly from the polymer surface (see Shi, H. & Ratner, B.D. Template recognition of protein-imprinted polymer surfaces. J. Biomed. Mater. Res., 2000, 49 :1-11), but the polymer surface area is too small to effectively recognize and separate macromolecules

Method used

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  • RNA blot electro-response hydrogel and its preparing method

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Embodiment 1

[0021] Embodiment 1: A kind of Northern blot electroresponsive hydrogel, it is characterized in that: 1) template macromolecule is yeast type VI RNA; (2) hydrogel is 2-acrylic acid amino-2-methyl-1-propane Sulfonic acid and N, N'-methylenebisacrylamide copolymer; (3) The hydrogel has a cross-linked structure.

[0022] The aforementioned Northern blot electro-responsive hydrogel is insoluble in any solvent.

[0023] The aforementioned Northern blot electro-responsive hydrogel can wash out part of the template macromolecules from the hydrogel under the action of an electric field.

[0024] A method for preparing Northern blotting electroresponsive hydrogel, characterized in that it consists of the following steps:

[0025] 1. Preparation of polymer:

[0026] Add 3 g of 2-acrylic acid amino-2-methyl-1-propanesulfonic acid and 1 g of N, N'-methylenebisacrylamide in 5 mL of 60 μg / mL aqueous solution of yeast type VI RNA; Incubate at ℃ for 1 h to orient the monomers before polyme...

Embodiment 2

[0033] Embodiment 2: A kind of Northern blot electroresponsive hydrogel, it is characterized in that: (1) template macromolecule is yeast VI type RNA; (2) hydrogel is 2-acrylic acid amino-2-methyl-1- Propanesulfonic acid and N, N'-methylenebisacrylamide copolymer; (3) The hydrogel has a cross-linked structure.

[0034] The aforementioned Northern blot electro-responsive hydrogel is insoluble in any solvent.

[0035]The aforementioned Northern blot electro-responsive hydrogel can wash out part of the template macromolecules from the hydrogel under the action of an electric field.

[0036] A method for preparing Northern blotting electroresponsive hydrogel, characterized in that it consists of the following steps:

[0037] 1. Preparation of polymer:

[0038] Add 5 g of 2-acrylic acid amino-2-methyl-1-propanesulfonic acid and 2 g of N, N'-methylenebisacrylamide in 10 mL of 60 μg / mL aqueous solution of yeast type VI RNA; Incubate at ℃ for 2 hours to use RNA molecules to orient ...

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Abstract

Barm VI type RNA template macromolecules are embedded in a copolymer of 2-crylic acid amido-2-methyl-1-propane sulfonic and N, N'-methylene bisacrylamide, a hydrogel can urge the macromolecules eluted from the polymer since the hydrogel can expand and shrink inversely under the action of an outer field while the print of the template macromolecules left in the gel can identify barm VI type RNA.

Description

(1) Technical field: [0001] The invention belongs to molecular imprinting technology, in particular to a ribonucleic acid (RNA) imprinted electroresponsive hydrogel and a preparation method thereof, which uses a ribonucleic acid (RNA) biomacromolecule as a template to imprint a polymer, and to solve It provides a method for the problem that the template is difficult to come out in the biomacromolecule imprinting technology. (two) background technology: [0002] Molecular imprinting technology (see Wulff, G. Molecular imprinting in cross-linked materials with the aid of molecular template a way towards artificial antibodies. Angew. Chem. Int. Ed., 1995, 34: 1821-1832) is to dissolve template small molecules in In monomers, when the monomers are polymerized and cross-linked, the template molecules are embedded in the polymer network. Then, the template molecules are washed away from the polymer, and a large number of cavities complementary in shape to the template molecules a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C08F220/04C08J5/00
Inventor 黄积涛郑嗣华张嘉琪谢秀荣黄卫洪
Owner TIANJIN UNIVERSITY OF TECHNOLOGY