Sensor for detecting phenolic substance in compost and detection method thereof

A phenolic substance and sensor technology, which is applied in the field of biosensor determination of phenolic substances in complex composting systems

Inactive Publication Date: 2008-04-30
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims to use the principle of electrochemistry to provide an amperometric biosensor for measuring the content of phenols, so as to improve the sensitivity and anti-interference ability of the determination of phenolic substances, so that it can be better applied to the composting of municipal solid waste and solve the problem of composting. Real-time On-line Monitoring Problems in Process Control System

Method used

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  • Sensor for detecting phenolic substance in compost and detection method thereof
  • Sensor for detecting phenolic substance in compost and detection method thereof
  • Sensor for detecting phenolic substance in compost and detection method thereof

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Experimental program
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Effect test

Embodiment Construction

[0042] 1. Laccase-Fe 3 o 4 Preparation of magnetic nanoparticle cross-linked body

[0043] Under the protection of nitrogen, a certain amount of Fe 2+ : Fe 3+ = 1:2 ratio of FeCl 2 , FeCl 3 Add 38mL 0.4M hydrochloric acid to dissolve, then pour into 375mL 0.7M ammonia water, stir vigorously to form Fe 3 o 4 Colloidal precipitate. Then take 0.232g of the precipitate, add it to 200mL of n-propanol, and then add 5.36g of PEG (polyethylene glycol) and 20mL of H 2 O, 10mL ammonia water and 1.2mLTEOS (tetraethyl orthosilicate), stirred for 24h, separated, washed, and freeze-dried, suspended in 10mL methanol with 0.5g, added 0.5mL APTMS (aminopropyltrimethoxysilane), stirred at room temperature Silanization was completed within 12 hours, and then suspended in 5 mL of 2.5% glutaraldehyde and stirred to obtain aldylated core / shell magnetic nanoparticles. Then at 4 °C, 0.01 g laccase and 0.5 g magnetic nanoparticles were added to 5 mL of 1 / 15M Na 2 HPO 4 and 1 / 15MKH 2 PO 4 ...

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Abstract

This invention relates to a sensor for detecting phenolic substance in compost and detection method thereof. With the carbon paste electrode modified with laccase-Fe3O4 magnetic nano grain as the work electrode to detect the response current variation of compost leachate in tri-electrode electrolytic bath; with given regression equation that is linear for hydroquinone of 1*10-7~1.375*10-4M and pyrocatechin of 5*10-8~2.75*10-4M, and computing the phenol content in compost. This invention is faster than HPLC and overcomes the defects in spectrophotometry, and has wide application for domestic garbage compost control system.

Description

technical field [0001] The invention relates to a detection method of phenol content, in particular to a biosensor measurement of phenolic substances in a complex composting system. Background technique [0002] Phenolic substances are very important basic raw materials and by-products in the large-scale organic chemical industry. They are widely present in the environment, difficult to be degraded biologically or abiotically, and have high toxicity. As a recognized harmful substance, phenol has a great impact on human health and the environment. In the municipal solid waste composting system, there are a variety of phenolic pollutants, and various enzymes such as lignin peroxidase, manganese peroxidase, laccase and polyphenol oxidase secreted by natural microbial flora in the compost Both can catalyze the detoxification and oxidation of phenolic substances. Therefore, the detection of phenolic substances in the composting process can implement the monitoring and control o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G06F19/00
Inventor 曾光明汤琳章毅牛承岗沈国励黄丹莲
Owner HUNAN UNIV
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