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Dog-like sperm super-low temperature freezing preservation antifreeze solution

An ultra-low temperature freezing and antifreeze technology, which is used in the preservation of human or animal bodies, medical raw materials derived from mammals, animal husbandry, etc., to achieve the effects of improving the conception rate, simple preparation and easy promotion.

Inactive Publication Date: 2008-05-14
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is also reported that N-acetyl-D-glucosamine has been successfully applied in porcine semen frozen dilution (Yi et al., 2002, Animal Reproduction Science.74, 187-194.), but about OEP and N-acetyl-D -The study of glucosamine used in canine semen antifreeze has not been reported yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1 (antifreeze 1)

[0055] First weigh 0.06g of N-acetyl-D-glucosamine, dissolve it in 10ml of distilled water, and dissolve it completely. Then measure 20 mL of egg yolk, 3 mL of glycerin, and 0.50 mL of amino-sodium-dodecyl sulfate (OEP) and mix them uniformly. Then weigh 1.20g of fructose and dissolve it in 50ml of distilled water. After it is completely dissolved, add 3.15g of trishydroxymethylaminomethane and 1.65g of citric acid, and add them one by one in the above order. Add the latter, mix the three preparations after dissolving, then add 100,000 units of penicillin or gentamycin, and finally distilled water to a volume of 100ml. ℃ refrigerator. All preparation processes are carried out under sterile conditions. This formula has an excellent effect on the resistance to low temperature damage of canine semen during cryopreservation at ultra-low temperature. When using it, mix it with canine semen at a ratio of 1:3, and keep the antifreeze at the sa...

Embodiment 2

[0057] Embodiment 2 (antifreeze 2)

[0058] First weigh 0.05g of N-acetyl-D-glucosamine, dissolve it in 10ml of distilled water, and dissolve it completely. Then measure 20 mL egg yolk, 4 mL dimethyl sulfoxide (DMSO), 0.75 mL amino-sodium-dodecyl sulfate (OEP) and mix them uniformly. Then weigh 1.65g of fructose and dissolve it in 50ml of distilled water. After it is completely dissolved, add 1.85g of trishydroxymethylaminomethane and 2.25g of citric acid, and add them one by one in the above order. Add the latter, mix the three preparations after dissolving, then add 100,000 units of penicillin or gentamicin, and finally distilled water to a volume of 100ml. ℃ refrigerator. All preparation processes are carried out under sterile conditions. This formula has an excellent effect on the resistance to low temperature damage of canine semen during cryopreservation at ultra-low temperature. When using it, mix it with canine semen at a ratio of 1:3, and keep the antifreeze at th...

Embodiment 3

[0060] Embodiment 3: (antifreeze solution 3)

[0061] First weigh 0.05g of N-acetyl-D-glucosamine, dissolve it in 10.0ml of distilled water, and dissolve it completely. Then measure 20.0 mL of egg yolk, 6.0 mL of glycerin, and 0.70 mL of amino-sodium-dodecyl sulfate (OEP) and mix them uniformly. Then weigh 2.65g of fructose and dissolve it in 50ml of distilled water. After completely dissolving, add 1.15g of trishydroxymethylaminomethane and 0.95g of citric acid, and add them one by one in the above order. Add the latter, mix the three preparations after dissolving, then add 100,000 units of penicillin or gentamicin, and finally distilled water to a volume of 100ml. ℃ refrigerator. All preparation processes are carried out under sterile conditions. This formula has an excellent effect on the resistance to low temperature damage of canine semen during cryopreservation at ultra-low temperature. When using it, mix it with canine semen at a ratio of 1:3, and keep the antifreez...

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PUM

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Abstract

The present invention relates to a canine sperm ultra-low temperature cold storage anti-freezing solution, belonging to the field of animal propagation technology. It is prepared by using amino-sodium-dodecyl sulfate (OEP) 0.05-2.50 ml, N-acetyl-D-glucose amine 0.01-0.10g, fluctose 0.45-3.10g, citric acid 0.50-2.30g, tris (hydroxy methyl) aminomethane 1.10-3.90g, egg yolk 15.0-30.0 ml, glycerine or dimethyl sulphoxide 2.0-8.0 ml, penicillin or gentamycin antimicrobial 1000 UI / ml and distilled water added to 100 ml. The pH value of said antifreezing solution is 6.25-6.95, and its osmotic pressure is 300-350 mOsmo / L.

Description

technical field [0001] The invention relates to an antifreeze solution for ultra-low temperature cryopreservation of canine sperm, which is used for ultra-low temperature cryopreservation of canine sperm and artificial insemination breeding by using frozen semen, and belongs to the technical field of animal reproduction. Background technique [0002] As the earliest domesticated livestock, dogs play a unique role in human production and life, and the social demand for fine-bred dogs is increasing day by day. The breeds of dogs in my country are not only famous in the world for their unique use and ornamental value, but also have an important impact on the formation of world-famous breeds, such as Tibetan Mastiff (Mastiff), Shih Tzu, Pekingese, and Tibetan Lhasa Dog (Egyptian Dog). Puso dog), etc., as the breed resources of precious dogs, have become the common wealth of the people of the world. With the improvement of economic conditions in my country's urban and rural resid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/00A61K35/52
Inventor 李新红
Owner SHANGHAI JIAOTONG UNIV