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Pyridoxal phosphate internally cross-linked hematoglobin nanocapsule and its preparing method

A technology of pyridoxal phosphate and hemoglobin, which is applied in peptide/protein components, blood diseases, capsule delivery, etc., can solve the problems of high cost, inability to further promote encapsulated hemoglobin products, complicated operation, etc., and achieve good biocompatibility , good oxygen carrying capacity, simple preparation method

Active Publication Date: 2008-05-28
SHANGHAI BLOOD CENT
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Problems solved by technology

[0009] At present, some literatures have reported the method of encapsulating hemoglobin with biodegradable materials, but these methods cannot be further extended to industrial production of encapsulated hemoglobin products due to the need for special equipment, complicated operation and high cost.

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  • Pyridoxal phosphate internally cross-linked hematoglobin nanocapsule and its preparing method
  • Pyridoxal phosphate internally cross-linked hematoglobin nanocapsule and its preparing method
  • Pyridoxal phosphate internally cross-linked hematoglobin nanocapsule and its preparing method

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Embodiment Construction

[0033] The above content of the present invention will be further described in detail below in the form of specific embodiments, but it should not be understood that the scope of the above subject matter of the present invention is only limited to the following specific embodiments.

[0034] 1. Purification of Hemoglobin

[0035]Take a bag of expired whole blood in stock, pack it into 50ml plastic centrifuge tubes, and centrifuge at 3500rpm for 10min. Aspirate the plasma part with a glass dropper, add an equal volume of normal saline and mix well, then centrifuge at 3500rpm for 10min, repeat the above steps 3 times. The washed erythrocytes obtained above were added to water at a volume ratio of 1:9, mixed evenly, and placed in a refrigerator at 4° C. overnight. The above hemolysis was centrifuged at 10000rpm (8600g) for 50min. Take the supernatant hemoglobin solution and discard the precipitate. The supernatant solution was sterilized and filtered at 0.22 μm and stored in a...

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Abstract

The present invention relates to nanometer material coated internally cross-linked hematoglobin nanocapsule and its preparation process. The pyridoxal phosphate internally cross-linked hematoglobin nanocapsule includes nanometer chitosan and sodium alginate as coating material and pyridoxal phosphate internally cross-linked hematoglobin, has diameter of 60-120 nm, and possesses excellent oxygen carrying capacity, excellent biocompatibility and no adverse effect on various blood components. The preparation process uses no organic solvent and easy-to-decompose nanometer coating, resulting in no toxicity. The preparation process is simple, fast and expected for large scale application.

Description

technical field [0001] The invention relates to the technical field of a nanocapsule which uses nanomaterials to wrap internally cross-linked hemoglobin and a preparation method thereof. Background technique [0002] Blood is an important part of the human body, a large amount of blood loss will cause serious metabolic disorders and even life-threatening, blood transfusion has become an indispensable clinical first aid measures and medical means. In recent years, the demand for blood transfusion is increasing all over the world. However, due to the complex blood types of human beings, transfusion reactions are difficult to overcome; the storage period of natural blood is short, coupled with the threat of transfusion-transmitted infectious diseases and the shortage of blood donors, safe and effective blood sources are increasingly available. In short supply. Therefore, human blood substitutes have become a research hotspot of international concern and have been listed as a m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/42A61K47/36A61K9/50A61P7/08
Inventor 范华骅
Owner SHANGHAI BLOOD CENT
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