Unlock instant, AI-driven research and patent intelligence for your innovation.

Organic microbial composite and use

A microbial composition and organic technology, applied in the biological field, can solve the problems of trace elements and nutrient loss, harmful chemical residues exceeding the standard, and the quality of agricultural products deteriorating, and achieve the effects of improved taste, low production cost, and improved quality

Inactive Publication Date: 2008-08-06
利耀铭
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] With the in-depth study of microbial application technology and the application of industrial production, the application technology of microorganisms has developed by leaps and bounds, especially when people realize that the large-scale application of chemical fertilizers and pesticides will cause environmental pollution and the impact on the quality of agricultural products. Long-term excessive The use of chemical fertilizers and pesticides has caused soil compaction, an imbalance in the ecological environment, serious excess of harmful chemical residues, and water pollution, resulting in a decline in the quality of agricultural products, poor taste and taste, and the loss of various trace elements and nutrients.
Similarly, the feed for poultry and livestock produced by these crops cannot make the poultry and livestock grow healthily after eating, so hormones, antibiotics and other means are used to form a vicious circle, and finally the agricultural products of our country are at a low level for a long time. , low prices, and exports are restricted by increasingly high barriers

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Organic microbial composite and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The source, cultivation and preservation of embodiment 1 Saccharomyces cerevisiae Li1012 (Saccharomyces cerevisiae Li1012)

[0037] Source: wild yeast isolated and selected from the soil of litchi orchards planted in Baiyun District, Guangdong.

[0038] It was identified as Saccharomyces cerevisiae Li1012 cultured by China Center for Typical Culture Collection, Wuhan University, China:

[0039] The medium used: peel the potatoes, cut them into small pieces, add water to boil for 30-45 minutes, filter, mix the filtered potatoes with glucose and water according to the weight ratio of 10:1:50, and sterilize under an atmospheric pressure for 18-45 minutes. 30 minutes.

[0040] Strain cultivation: Saccharomyces cerevisiae Li1012 was inoculated into the above-mentioned medium, and cultured in an incubator at 32-35° C. for 3-5 days to obtain yeast strains.

[0041] Strain preservation:

[0042] Slant medium formula: yeast extract 0.5-0.8%, peptone 1.0-1.3%, glucose 0.5-0.8%...

Embodiment 2

[0044] The preparation of embodiment 2 organic microbial composition

[0045] Step 1: (1) Enlarged culture of pure species: Weigh 1000g of bran and 700g of water, mix well and make solid medium A, pack in 500ml Erlenmeyer flasks, each bottle contains 300g of medium A, sterilize at 0.1MPa for 30 Take the strains of Trichoderma, Aspergillus, Actinomycetes, and Bacillus, respectively, and inoculate them in the Erlenmeyer flasks with medium under sterile conditions, place them in an incubator for cultivation, and cultivate them at 30-33°C for 72 hours ;

[0046] (2) Liquid fermentation culture:

[0047] Weigh 5g of yeast extract, 10g of peptone, 8g of glucose, 12g of white granulated sugar, and 965g of water, stir and dissolve, then the pH value is 6.5 as a liquid medium, and divide them into 500ml triangular flasks, 250ml per bottle, and sterilize at 0.1MPa for 30 minutes , take the Saccharomyces cerevisiae strain of Example 1 and inoculate it in a Erlenmeyer flask equipped wit...

Embodiment 3

[0051] The preparation of embodiment 3 organic microbial composition

[0052] (1) Enlarged cultivation of pure species: Weigh 2000g of bran and 1500g of water, stir and mix it as solid medium A, divide it into 500ml Erlenmeyer flasks, put 250g of medium A in each bottle, and sterilize at 0.1MPa for 30 minutes. The strains of Trichoderma, Aspergillus, Actinomycetes, and Bacillus were respectively inoculated in the Erlenmeyer flasks with medium under aseptic conditions, placed in an incubator for cultivation, and cultivated at 30-33°C for 48 hours;

[0053] (2) Liquid fermentation culture:

[0054] Take by weighing 80g of yeast extract, 130g of peptone, 50g of glucose, 100g of white granulated sugar, and 9640g of water after stirring and dissolving, the pH value is 6.5 as a liquid medium, put it in a 5L fermenter, and sterilize it at 0.1MPa for 30 minutes. Take Example 1 The Saccharomyces cerevisiae strains were inoculated under aseptic conditions, controlled at 30-32° C. under...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

This invention provides an organic microorganism composite and its usage, and it relates to biotechnology field. The strain of this composite includes trichoderma aspergillus, actinomycetes, bacillus and saccharomyces cerevisiae Li1012. The composite whose bacterial number, yeast count and mycetes number are 1.0-4.0*108cfu / g, 3.0-8.0*105cfu / g and 2.0-5.5*106cfu / g is prepared after the process of pure mass-cultivation, liquid fermentation and solid fermentation, with its water content lower than 10%, which can be used in producing organic fertilizer or forage additives. It will be of great contribution towards the health of crops when it is used for fertilizer preparation.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an organic microbial composition containing active microbial flora and its use. Background technique [0002] With the in-depth study of microbial application technology and the application of industrial production, the application technology of microorganisms has developed by leaps and bounds, especially when people realize that the large-scale application of chemical fertilizers and pesticides will cause environmental pollution and the impact on the quality of agricultural products. Long-term excessive The use of chemical fertilizers and pesticides has caused soil compaction, an imbalance in the ecological environment, serious excess of harmful chemical residues, and water pollution, resulting in a decline in the quality of agricultural products, poor taste and taste, and the loss of various trace elements and nutrients. Similarly, the feed for poultry and livestock produced by the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/20C05F11/08A23K1/16C12R1/885C12R1/66C12R1/04C12R1/07C12R1/865A23K10/18
Inventor 利耀铭
Owner 利耀铭