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Apparatus and method for making gradient gel film for full-automatic electrophoresis

A technique for producing gradient gels and devices, which is applied in the field of automatic control production, production equipment for biochemical detection materials, and fully automatic production devices for electrophoretic gradient gel films. To obtain continuous and stable quality gel products, and the parameters of gradient gel sheets vary greatly, so as to achieve the effects of good quality and consistency, reduced production costs, and improved resolution

Inactive Publication Date: 2008-08-20
XIAN POLYTECHNIC COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the domestic gradient film produced by special equipment has not yet been seen.
[0009] At present, foreign equipment is expensive for the production of gels, while the manual production method is still used in China, which is not only time-consuming and laborious, but also difficult to produce, and the parameters of the produced gradient gel slices are very different, which directly affects the test accuracy.
It can also be said that the manual production method cannot obtain guaranteed continuous and stable quality gel products, nor can it achieve automation, high quality and high efficiency.

Method used

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  • Apparatus and method for making gradient gel film for full-automatic electrophoresis
  • Apparatus and method for making gradient gel film for full-automatic electrophoresis
  • Apparatus and method for making gradient gel film for full-automatic electrophoresis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Such as figure 2 As shown, the automatic production device for electrophoretic gradient gel film includes a container and a film box, and the single-set glue pouring device also includes a single-chip microcomputer control system 3, a glue filling system and a film box support device, and a single-chip microcomputer control system 3 The output control signal is connected to the signal input end of the glue filling system through the cable 4 to control the feed control of the stepping motor 5 in the glue filling system for water filling and glue filling. The glue filling system is controlled by the base 6 , stepping motor 5, glue filling device mechanical transmission device 7, glue filling device 8, infusion catheter 10, water container, glue container, glue liquid agitator 12 and electromagnetic liquid flow valve 9 constitute, stepping motor 5, filling The glue dispenser 8 is installed on the base 6 through the mechanical transmission device 7 of the glue dispenser, t...

Embodiment 2

[0051] Such as figure 1 As shown, the overall composition is the same as in Example 1. The present invention adopts the computer control system for the production process of the gradient electrophoresis gel sheet in the overall scheme, and adopts the design of distributed control of the upper and lower computers, and the upper computer is mainly used for data Operation, the lower computer uses a single-chip microcomputer system for the control of the production process. One upper computer can control multiple lower computers, and the lower computers combine corresponding machinery and control devices to greatly improve the automation of gradient electrophoresis film production, making the production process easy to operate, and the film quality and consistency good.

[0052] The system adopts computer control and management. The computer control management software has the functions of film quantity setting, film concentration setting, data calculation, glue filling machine nu...

Embodiment 3

[0055] Such as image 3 As shown, the overall composition is the same as in Embodiment 1. The single-chip microcomputer control system 3 of the present invention is composed of a central processing unit and its peripheral circuits. The peripheral circuits include a display drive circuit, a display, a chip selection circuit, a keyboard, a serial communication interface circuit, and Watchdog circuit, photocoupler, DC motor and stepper motor driver, relay, required power supply, single chip microcomputer I / O port and display drive circuit signal connection, the output signal of the display drive circuit is connected to the signal input terminal of the display, on-chip The output signal of the selection circuit is connected to the trigger terminal of the display drive circuit. The keyboard is connected to the I / O interface circuit of the central processing unit, the serial communication interface circuit performs two-way data exchange with the central processing unit, and the watc...

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PUM

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Abstract

The invention discloses a film making device and preparing method of full-automatic electrophoretic gradient gel, which is characterized by the following: the gradient gel making course is controlled by computer automatically with upper and lower set matching the distribution control; the encapsulating device contains computer, single-chip set control system, matching and encapsulating system and film box support device, wherein the matching and encapsulating system connects the film box device through three-way pipe microblender pipe; when preparing small amount of film, the encapsulating device is controlled by a case of single-chip set; when preparing large amount of film, the computer controls 1-20 cases of encapsulating device. The invention solves the preparing difficulties of gradient gel film, which improves the quality control and consistency of batched preparation.

Description

technical field [0001] The invention relates to automatic control processing of biochemical materials, and mainly relates to production equipment and automatic control production method of biochemical detection materials. The density of the produced electrophoresis gel film is distributed according to a certain gradient. Specifically, it is an automatic production device and a production method of an electrophoretic gradient gel film. Background technique [0002] Electrophoresis refers to the charged test substance (protein, nucleotide, etc.) in an inert support medium (such as paper, cellulose acetate, agarose gel, polyacrylamide gel, etc.), under the action of an electric field , to the direction of the corresponding electrode according to their respective speeds, so that they are separated into narrow zones, and the method of recording the electrophoretic zone map or calculating its percentage content with a suitable detection method. Gradient gel film is used in gene s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/453G01N27/00B01D57/02
Inventor 倪原白玉杰郭晏海高嵩
Owner XIAN POLYTECHNIC COLLEGE