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Human lysozyme suppository, preparation method and application

A technology of lysozyme suppository and human lysozyme, which is applied in the field of biopharmaceuticals, achieves the effect of good medicinal prospect, simple preparation process and mature preparation method

Active Publication Date: 2008-09-24
安米
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, Chinese patent 03110824.5 discloses the use of human lysozyme against drug-resistant bacteria, but whether there is a more reasonable and effective dosage form for certain diseases, and there is still a big gap in the development of preparation and treatment of more diseases

Method used

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  • Human lysozyme suppository, preparation method and application
  • Human lysozyme suppository, preparation method and application
  • Human lysozyme suppository, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] The first step, the preparation of recombinant human lysozyme: the recombinant human lysozyme is based on the preparation of 200 ml of medium, with 6 ml of phosphoric acid, 3 grams of magnesium sulfate, 4 grams of potassium sulfate, 1 gram of potassium hydroxide, and 1.5 grams of calcium sulfate. gram, add distilled water to 200 milliliters, inoculate glycerin tube seeds after autoclaving, shaker rotation speed is 250 revolutions per minute, culture temperature is 20-35 ℃, cultivate 36-48 hours on constant temperature bed. The seed tank culture is carried out, and finally the production tank culture is carried out. Extract and purify the culture medium that has been fermented and expressed, freeze-dry the extracted and purified protein concentrate, measure the protein and store it for activity.

[0099]The second step, suppository preparation: 3000U / mL was prepared from recombinant human lysozyme with a purity of 95%, phosphate buffer 12mM (pH6.5-7.5), 75% propylene gly...

Embodiment 2

[0101] According to the method described in Example 1, in a pharmaceutical factory meeting the GMP requirements, 1 million U / mL of recombinant human lysozyme with a purity of 95% was produced according to pharmaceutical regulations, and phosphate buffer 15mM (pH6.5~7.5), 80% propylene glycol, 5 / 10,000 Tween 80, and 15% gelatin are made into suppositories.

Embodiment 3

[0103] According to the method described in Example 1, in a pharmaceutical factory meeting the GMP requirements, 2,000,000 U / mL of recombinant human lysozyme with a purity of 95% was produced according to pharmaceutical regulations, and phosphate buffer 20mM (pH6.5~7.5), 85% propylene glycol, 5 / 10,000 Tween 80, and 15% gelatin are made into suppositories.

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Abstract

The invention relates to the use of human lysozyme in biological medicines targeting drug resisting bacterium and viruses, the invention also discloses its preparation and use thereof. The human lysozyme film agent contains active 300U - 3 million U / ml of human lysozyme, the suppository prepared thereby can be administered directly through rectal intestine, thus can prevent the gastric mucosa from being stimulated, it can facilitate administration to infant and children by using this method.

Description

Technical field: [0001] The invention relates to biopharmaceuticals, in particular to human lysozyme medicine for anti-drug-resistant bacteria and viruses, its preparation method and application. Background technique: [0002] Today in the 21st century, the development of drug-resistant bacteria is shocking. The so-called drug-resistant bacteria are bacteria that develop resistance to drugs. After the bacteria have been in contact with the drug for many times, their sensitivity to the drug decreases or even disappears, resulting in a decrease in the sensitivity of the drug to the bacteria or even ineffectiveness. Among them, drug-resistant bacteria include Staphylococcus aureus MRSA, Staphylococcus epidermidis MRSE, Streptococcus pneumoniae, Streptococcus hemolyticus, Enterococcus, Escherichia coli, Klebsiella pneumoniae, Propionibacterium, Neisseria gonorrhoeae, Citronella Acidobacillus, Serratia, Enterobacter cloacae, Pseudomonas aeruginosa, Candida albicans, etc. Mainly...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/48A61K9/02A61P31/04A61P31/12A61P15/02A61P13/02A61P1/00
Inventor 安米
Owner 安米
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