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Method for sterilizing fermenter

A technology of disinfection and fermentation tanks, which is applied in heating and other directions, can solve the problems of large steam consumption, low utilization rate of equipment, and long non-production time, so as to save energy, reduce time and labor workload, and ensure aseptic effect Effect

Inactive Publication Date: 2009-09-30
LINGHUA GROUP INCORPORATED COMPANY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In the traditional fermentation process, such as glutamic acid, glutamine, lysine and other fermentation products, in order to ensure the smooth progress of the fermentation process and eliminate the contamination and economic losses caused by equipment, the continuous disinfection and sterilization method of fermentation broth is required The fermenter is cleaned with lye before each batch of feed, and then sterilized by steam and high pressure. This disinfection method not only consumes a lot of steam, but also has a lot of non-production time for the equipment, and the utilization rate of the equipment is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Embodiment 1: The fermentation tank sterilization method, in the case of continuous disinfection and sterilization of the fermented liquid, changes the disinfection mode of the fermentation tank, reduces the number of steam disinfection tanks, saves steam, and steams the fermentation tank after multiple batches of fermentation. Bacteria once, that is, each batch of fermentation tanks is only fed with lye (100°C, 25 minutes) for circular cleaning, and then steam is used for high-pressure sterilization (120°C, 0.20MP a , 40 minutes), also achieve the original effect of disinfection and sterilization of each batch of fermentation tanks. The operation process of the present invention is: fermentation finishes → fermenter disinfection (100 ℃, 25 minutes of each batch of lye) carries out circulation cleaning, every 3 batches of high-pressure steam sterilization (120 ℃, 0.20MP a , 40 minutes)→feeding→fermentation.

Embodiment 2

[0009] Embodiment 2: The fermenter sterilization method, in the case of continuous sterilization of the fermented liquid, changes the sterilization method of the fermenter, reduces the number of times of steam sterilization, saves steam, and steams the fermenter once after multiple batches of fermentation The method, that is, each batch of fermentation tanks is only fed with lye (105°C, 20 minutes) for circular cleaning, and after 4 batches of operation, steam is used for high-pressure sterilization (125°C, 0.18MP a , 35 minutes), also reach the original effect of disinfection and sterilization of each batch of fermentation tanks. The operation process of the present invention is: fermentation finishes → fermenter disinfection (105 ℃, 20 minutes of each batch of lye) carries out circulation cleaning, every 4 batches of high-pressure steam sterilization (125 ℃, 0.18MP a , 35 minutes)→feeding→fermentation.

Embodiment 3

[0010] Embodiment 3: The fermenter sterilization method, in the case of continuous sterilization of the fermented liquid, changes the sterilization method of the fermenter, reduces the number of times of steam sterilization, saves steam, and steams the fermenter once after multiple batches of fermentation The method, that is, each batch of fermenter tanks is only fed with lye (95°C, 30 minutes) for circular cleaning, and then steam is used for autoclaving after 5 batches of operation (122°C, 0.22MP a , 45 minutes), also achieve the original effect of disinfection and sterilization of each batch of fermentation tanks. The operation process of the present invention is: fermentation finishes → fermenter disinfection (95 ℃, 30 minutes of each batch of lye) carries out circulation cleaning, every 5 batches of high-pressure steam sterilization (122 ℃, 0.22MP a , 45 minutes)→feeding→fermentation.

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Abstract

The invention provides a disinfection and sterilization method for a fermenter, belonging to the technical field of disinfection and sterilization. The method is to change the cycle cleaning of each batch of lye in the fermenter (95°C to 105°C, 20 to 30 minutes) under the condition of continuous elimination of fermentation liquid. ) and steam sterilization (120 ℃ ~ 125 ℃, 0.18 ~ 0.22MPa, 35 ~ 45 minutes), only lye circulating cleaning is performed for each batch, and steam sterilization is performed after every 3-5 batches (120 ℃~125℃, 0.18~0.22MPa, 35~45 minutes) new method. Compared with the prior art, the method for sterilizing and sterilizing the fermenter of the present invention has the advantages of saving steam, shortening the non-fermentation time, etc., and also achieves the effect of sterilizing and sterilizing each batch of the original fermenter.

Description

(1) Technical field [0001] The invention relates to a disinfection and sterilization technology, in particular to a new method for disinfection and sterilization of fermenters in use. (2) Technical background [0002] In the traditional fermentation process, such as glutamic acid, glutamine, lysine and other fermentation products, in order to ensure the smooth progress of the fermentation process and eliminate the contamination and economic losses caused by equipment, the continuous disinfection and sterilization method of fermentation broth is required The fermenter is cleaned with lye before feeding each batch, and then sterilized by steam and high pressure. This sterilization method not only consumes a lot of steam, but also has more non-production time of the equipment, and the utilization rate of the equipment is low. (3) Contents of the invention [0003] The technical task of the present invention is to provide a fermentation tank disinfection and sterilization meth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L2/07
Inventor 杨玉岭岳希金邵明金满德恩胡昆之马志刚
Owner LINGHUA GROUP INCORPORATED COMPANY