Muskrat fragrant active component prepared by external secretion, preparing process and use

A technology of active components and preparation process, which is applied in the direction of essential oil/fragrance, drug combination, tissue culture, etc., and can solve the problems of lack of natural animal flavor resources and uncoordinated quantity ratio

Inactive Publication Date: 2007-08-15
陈玉山
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The resources of natural animal flavors are very scarce, and their quantity ratio is extremely inconsistent with plant flavors and synthetic flavors

Method used

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  • Muskrat fragrant active component prepared by external secretion, preparing process and use
  • Muskrat fragrant active component prepared by external secretion, preparing process and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0157] 1. Primary culture (tissue block culture):

[0158] 1. Material collection: use sterilized dissecting scissors to cut out the scent gland tissue, put it into a sterilized petri dish, wash it with PBS (sodium chloride 8.0g, potassium chloride 0.2g, disodium hydrogen phosphate 1.15g, potassium dihydrogen phosphate 0.2g, add water to make up to 1000ml) and rinse 3 times to remove blood stains.

[0159] 2. Isolate the tissue: Repeatedly cut the tissue into 1mm with sterilized ophthalmic scissors in a petri dish 2 The small pieces were cleaned by pipetting with PBS.

[0160] 3. Inoculation: Add 2 drops of primary culture solution (RPMI1640 85%, calf serum 10%, PHA 0.1ml 3%, heparin 10u / ml 2%, double antibody 500u / ml with a straw, and distribute them in 10ml culture bottles , 5ml of culture solution per bottle. Add about 0.5ml of fresh blood to each 5ml of culture solution.), use the tip of the elbow pipette to gently blow the tissue block evenly to make a suspension, and t...

Embodiment 2

[0171] 1. Primary culture (tissue block culture):

[0172] 1. Material collection: use sterilized dissecting scissors to cut out the scent gland tissue, put it into a sterilized petri dish, wash it with PBS (sodium chloride 8.0g, potassium chloride 0.2g, disodium hydrogen phosphate 1.15g, potassium dihydrogen phosphate 0.2g, add water to make up to 1000ml) and rinse twice to remove blood stains.

[0173] 2. Isolate the tissue: repeatedly cut the tissue into 0.5mm with sterilized ophthalmic scissors in a petri dish 2 The small pieces were cleaned by pipetting with PBS.

[0174] 3. Inoculation: Add 2 drops of primary culture solution (RPMI1640 85%, calf serum 10%, PHA 0.1ml 3%, heparin 10u / ml 2%, double antibody 500u / ml with a straw, and distribute them in 10ml culture bottles , 5ml of culture solution per bottle. Add about 0.5ml of fresh blood to each 5ml of culture solution.), use the tip of the elbow pipette to gently blow the tissue block evenly to make a suspension, and t...

Embodiment 3

[0185] 1. Primary culture (tissue block culture):

[0186] 1. Material collection: use sterilized dissecting scissors to cut out the scent gland tissue, put it into a sterilized petri dish, wash it with PBS (sodium chloride 8.0g, potassium chloride 0.2g, disodium hydrogen phosphate 1.15g, potassium dihydrogen phosphate 0.2g, add water to make up to 1000ml) and rinse 3 times to remove blood stains.

[0187] 2. Isolate the tissue: Repeatedly cut the tissue into 1mm with sterilized ophthalmic scissors in a petri dish 2 The small pieces were cleaned by pipetting with PBS.

[0188]3. Inoculation: Add 2 drops of primary culture solution (RPMI1640 85%, calf serum 10%, PHA 0.1ml 3%, heparin 10u / ml 2%, double antibody 500u / ml with a straw, and distribute them in 10ml culture bottles , 5ml of culture solution per bottle. Add about 0.5ml of fresh blood to each 5ml of culture solution.), use the tip of the elbow pipette to gently blow the tissue block evenly to make a suspension, and th...

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Abstract

The invention discloses a preparing method of muskrat fragrant active component with external secret scent, which comprises the following steps: supplying naphthene ketones with 7-21 carbon, fatty acid and ester, naphthenic alcohols with 3-33 carbon, aldehydes and alkenes; stripping fragrant gland bag of muskrat; shearing cortex part; washing with PBS; adding into original generation culture liquid; producing suspended liquid; feeding on the floor of culture bottle; adding into culture liquid to culture; choosing cell of fine and close step; adding into parenzyme; digesting; blowing cell single-layer slowly; sucking-off; transferring into another culture bottle; adding into culture liquid; culturing at constant temperature; extracting out of muskrat aromatic oil state matter; adding into parenzyme; reacting; adding into 1, 3 methyl glycol or dimethy ketone; stewing; separating; adding into kalii permangana; stewing with activated char; removing the kalii permangana; centrifuging; extracting with 1, 3 methyl glycol; removing solvent. This preparing craft is simple and stable, which is a new animal spice and medicinal material.

Description

Technical field: [0001] The present invention relates to an active ingredient of muskrat incense secreted in vitro, that is, prepared by cloning, a preparation process and its application, more precisely, it relates to an active ingredient of muskrat incense prepared from the secretion of muskrat sachets cultured in vitro , preparation process and use thereof. Background technique: [0002] Natural animal fragrances include musk, civet, beaver, ambergris and muskrat. Muskrat is another precious new resource of natural animal fragrances discovered through years of research after the four famous fragrances. The resources of natural animal flavors are very scarce, and their quantity ratio is extremely uncoordinated with plant flavors and synthetic flavors. Resources are extremely scarce. Natural musk is secreted by the glandular sacs of forest musk deer and horse musk deer, and there are very few countries where it is produced, and each musk deer produces only about 15 grams ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/00C12N5/06A61K35/55A61K31/215A61P9/10A61P7/02C11B9/00C12N5/071
Inventor 陈玉山赵伟刚魏海军赵景辉赵蒙
Owner 陈玉山
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