Muskrat fragrant active component prepared by external secretion, preparing process and use
A technology of active components and preparation process, which is applied in the direction of essential oil/fragrance, drug combination, tissue culture, etc., and can solve the problems of lack of natural animal flavor resources and uncoordinated quantity ratio
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Embodiment 1
[0157] 1. Primary culture (tissue block culture):
[0158] 1. Material collection: use sterilized dissecting scissors to cut out the scent gland tissue, put it into a sterilized petri dish, wash it with PBS (sodium chloride 8.0g, potassium chloride 0.2g, disodium hydrogen phosphate 1.15g, potassium dihydrogen phosphate 0.2g, add water to make up to 1000ml) and rinse 3 times to remove blood stains.
[0159] 2. Isolate the tissue: Repeatedly cut the tissue into 1mm with sterilized ophthalmic scissors in a petri dish 2 The small pieces were cleaned by pipetting with PBS.
[0160] 3. Inoculation: Add 2 drops of primary culture solution (RPMI1640 85%, calf serum 10%, PHA 0.1ml 3%, heparin 10u / ml 2%, double antibody 500u / ml with a straw, and distribute them in 10ml culture bottles , 5ml of culture solution per bottle. Add about 0.5ml of fresh blood to each 5ml of culture solution.), use the tip of the elbow pipette to gently blow the tissue block evenly to make a suspension, and t...
Embodiment 2
[0171] 1. Primary culture (tissue block culture):
[0172] 1. Material collection: use sterilized dissecting scissors to cut out the scent gland tissue, put it into a sterilized petri dish, wash it with PBS (sodium chloride 8.0g, potassium chloride 0.2g, disodium hydrogen phosphate 1.15g, potassium dihydrogen phosphate 0.2g, add water to make up to 1000ml) and rinse twice to remove blood stains.
[0173] 2. Isolate the tissue: repeatedly cut the tissue into 0.5mm with sterilized ophthalmic scissors in a petri dish 2 The small pieces were cleaned by pipetting with PBS.
[0174] 3. Inoculation: Add 2 drops of primary culture solution (RPMI1640 85%, calf serum 10%, PHA 0.1ml 3%, heparin 10u / ml 2%, double antibody 500u / ml with a straw, and distribute them in 10ml culture bottles , 5ml of culture solution per bottle. Add about 0.5ml of fresh blood to each 5ml of culture solution.), use the tip of the elbow pipette to gently blow the tissue block evenly to make a suspension, and t...
Embodiment 3
[0185] 1. Primary culture (tissue block culture):
[0186] 1. Material collection: use sterilized dissecting scissors to cut out the scent gland tissue, put it into a sterilized petri dish, wash it with PBS (sodium chloride 8.0g, potassium chloride 0.2g, disodium hydrogen phosphate 1.15g, potassium dihydrogen phosphate 0.2g, add water to make up to 1000ml) and rinse 3 times to remove blood stains.
[0187] 2. Isolate the tissue: Repeatedly cut the tissue into 1mm with sterilized ophthalmic scissors in a petri dish 2 The small pieces were cleaned by pipetting with PBS.
[0188]3. Inoculation: Add 2 drops of primary culture solution (RPMI1640 85%, calf serum 10%, PHA 0.1ml 3%, heparin 10u / ml 2%, double antibody 500u / ml with a straw, and distribute them in 10ml culture bottles , 5ml of culture solution per bottle. Add about 0.5ml of fresh blood to each 5ml of culture solution.), use the tip of the elbow pipette to gently blow the tissue block evenly to make a suspension, and th...
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