Method for preparing nitrogen contained syrup in use for ferment through wheat beta starch multienzyme method

A technology of B starch and amylase, which is applied in the field of producing nitrogen-containing syrup for fermentation by wheat B starch multi-enzyme method, can solve the problems of hindering refining, hindering filtration, strength, difficulty, etc., to increase water solubility and improve recovery rate , Reduce the formation and quantity of foam

Inactive Publication Date: 2011-07-06
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the purity of wheat B starch is low, the protein content exceeds 1%, even reaches 4-5%, and the content of other impurities such as pentosan and fat is also high. These impurities have a negative impact on the deep processing and application of wheat B starch
For example, in the preparation of modified starch, protein and pentosan will also participate in the modification reaction and hinder refining, and the denaturation is not easy to control; protein is also prone to color reaction, which affects the appearance and application of the product
If it is used to produce starch syrup, the pentosan in wheat B starch will produce viscosity, which seriously hinders the progress of filtration; protein is easy to produce air bubbles, flocs and water-soluble protein, which will also promote the production and deepening of the color of sugar solution. These impurities must be removed by means of precipitation, adsorption, filtration, ion exchange, etc., to ensure that the quality of starch syrup products meets national standards, and the strength, difficulty, and cost of refining are high.
Therefore, the use of wheat B starch is limited, and starch manufacturers generally can only sell it at a low price

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Slurry: Add appropriate amount of water in the slurry tank, start stirring, add wheat B starch, control the concentration of dry solids in the powder slurry to 25%, add calcium chloride, and control the Ca of the slurry 2+ content of 20mg / kg, adjust the pH value of the powder slurry to 5.9, and finally add 0.2kg / t of Liquozyme Supra high-temperature α-amylase, stir and mix evenly.

[0023] (2) Liquefaction: continuous jet liquefaction at 110°C, heat preservation treatment of liquefied liquid at 98-100°C for 140 minutes.

[0024] (3) Saccharification: Cool the liquefaction solution to 60°C, pH value is 4.1-4.3, add 0.6kg / t Dextrozyme DX glucoamylase for 40 hours, then add 0.001kg / t Shearzyme 2X pentosanase and lipase Hydrolysis, the total hydrolysis time is 70 hours, and the DE value of the product is 97.8.

[0025] (4) Protein conversion: After saccharification, control the temperature of the material at 45-50°C and the pH value at 6.5-6.8, add 0.005 kg / t of papain o...

Embodiment 2

[0028] (1) Slurry: Add appropriate amount of water to the slurry tank, start stirring, add wheat B starch, control the concentration of the dry solids of the powder slurry to 45%, add calcium chloride, and control the Ca of the slurry 2+ The content of 45mg / kg, adjust the pH value of the powder slurry to 6.4, finally add 1.2kg / t of Termamyl 120L high temperature α-amylase, stir and mix evenly.

[0029] (2) Liquefaction: continuous jet liquefaction at 108°C, heat preservation treatment of liquefied liquid at 98-100°C for 60 minutes.

[0030] (3) Saccharification: Cool down the liquefaction solution to 50°C, pH value is 5.6, add 1.5kg / t β-amylase, 0.05kg / t PromozymeD2 pullulan debranching enzyme to coordinate saccharification, time 15 hours, add 0.02kg / t of Shearzyme500L pentosanase and lipase for hydrolysis, and then hydrolyzed for 10 hours, the DE value of the product was 54.8.

[0031] (4) Protein conversion: the temperature of the material is raised to 60-65° C., the pH va...

Embodiment 3

[0034] (1) Slurry: Add appropriate amount of water to the slurry tank, start stirring, add wheat B starch, control the concentration of dry solids in the slurry to about 35%, add calcium chloride, and control the Ca of the slurry 2+ content of 40mg / kg, adjust the pH value of the powder slurry to 6.0-6.2, and finally add 0.8kg / t of Suhong AA Plus high-temperature α-amylase, stir and mix evenly.

[0035] (2) Liquefaction: Continuous jet liquefaction at 108-110°C, heat preservation treatment of liquefied liquid at 95-98°C for 140 minutes.

[0036] (3) Saccharification: Cool down the temperature of the liquefied liquid to 54-58°C, pH value is 4.1-4.4, add 1.0kg / t Suhong GA II or DextrozymeGA glucose glucoamylase for saccharification for 50 hours, add 0.01kg / t Shearzyme500L pentosanase , Finizym W lysophospholipase for hydrolysis, and then hydrolysis for 15 hours, the DE value of the product was 98.2.

[0037] (4) Protein conversion: the material temperature is 56-50°C, the pH val...

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PUM

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Abstract

The present invention discloses a process for preparing the N-contained syrup from the wheat starch B by multi-enzyme method, which can be used for fermentation, includes such steps as preparing the slurry, liquefying, saccharifying, protein converting, filtering and concentrating. The process features that: the starch, protein, pentosan and fat, which are the basic components of wheat starch B, are hydrolyzed by the amylase, pentosanase and lipase, thus apart from the obtained small quantity of faecal higher fatty acid with higher melting point, the protein which is not be hydrolyzed completely, and the added protease, the hydrolyzate and the proteolysis hydrolyzate of the added various kinds of amylase, dextranase and lipase are almost remained in the N-contained starch syrup . Therefore, the process for preparing the N-contained syrup from the wheat starch B by multi-enzyme method has the advantages of super-high output rate which is almost 100%, and the ability of being applied and carried out directly for the prior production line of preparing starch syrup by multi-enzyme method.

Description

technical field [0001] The invention relates to a method for preparing nitrogen-containing starch syrup used in the fields of food fermentation, biochemical pharmacy, etc., specifically, using amylase, protease, pentosanase and other enzymes to enzymolyze wheat B starch to prepare nitrogen-containing starch for fermentation Syrup method. Background technique [0002] Wheat is mainly used as food, and it is one of the main grains grown in large quantities all over the world, and it is also grown in large quantities in northern China. Wheat is dry-processed and ground into flour, and various pasta and pasta products made from flour processing have become the staple food of the people in the north. The main components of wheat flour (hereinafter referred to as flour) are starch, protein, ash, fat and water. After wheat flour is milled and sieved, there is almost no cellulose content. The (gluten) protein in wheat is known to have special properties that, of all grain flours, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/09C13K1/06C12P19/04A23L29/30
Inventor 黄立新徐正康赵谋明温其标周彦斌罗建勇许世枫
Owner SOUTH CHINA UNIV OF TECH
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