Method for preparing nitrogen contained syrup in use for ferment through wheat beta starch multienzyme method
A technology of B starch and amylase, which is applied in the field of producing nitrogen-containing syrup for fermentation by wheat B starch multi-enzyme method, can solve the problems of hindering refining, hindering filtration, strength, difficulty, etc., to increase water solubility and improve recovery rate , Reduce the formation and quantity of foam
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Embodiment 1
[0022] (1) Slurry: Add appropriate amount of water in the slurry tank, start stirring, add wheat B starch, control the concentration of dry solids in the powder slurry to 25%, add calcium chloride, and control the Ca of the slurry 2+ content of 20mg / kg, adjust the pH value of the powder slurry to 5.9, and finally add 0.2kg / t of Liquozyme Supra high-temperature α-amylase, stir and mix evenly.
[0023] (2) Liquefaction: continuous jet liquefaction at 110°C, heat preservation treatment of liquefied liquid at 98-100°C for 140 minutes.
[0024] (3) Saccharification: Cool the liquefaction solution to 60°C, pH value is 4.1-4.3, add 0.6kg / t Dextrozyme DX glucoamylase for 40 hours, then add 0.001kg / t Shearzyme 2X pentosanase and lipase Hydrolysis, the total hydrolysis time is 70 hours, and the DE value of the product is 97.8.
[0025] (4) Protein conversion: After saccharification, control the temperature of the material at 45-50°C and the pH value at 6.5-6.8, add 0.005 kg / t of papain o...
Embodiment 2
[0028] (1) Slurry: Add appropriate amount of water to the slurry tank, start stirring, add wheat B starch, control the concentration of the dry solids of the powder slurry to 45%, add calcium chloride, and control the Ca of the slurry 2+ The content of 45mg / kg, adjust the pH value of the powder slurry to 6.4, finally add 1.2kg / t of Termamyl 120L high temperature α-amylase, stir and mix evenly.
[0029] (2) Liquefaction: continuous jet liquefaction at 108°C, heat preservation treatment of liquefied liquid at 98-100°C for 60 minutes.
[0030] (3) Saccharification: Cool down the liquefaction solution to 50°C, pH value is 5.6, add 1.5kg / t β-amylase, 0.05kg / t PromozymeD2 pullulan debranching enzyme to coordinate saccharification, time 15 hours, add 0.02kg / t of Shearzyme500L pentosanase and lipase for hydrolysis, and then hydrolyzed for 10 hours, the DE value of the product was 54.8.
[0031] (4) Protein conversion: the temperature of the material is raised to 60-65° C., the pH va...
Embodiment 3
[0034] (1) Slurry: Add appropriate amount of water to the slurry tank, start stirring, add wheat B starch, control the concentration of dry solids in the slurry to about 35%, add calcium chloride, and control the Ca of the slurry 2+ content of 40mg / kg, adjust the pH value of the powder slurry to 6.0-6.2, and finally add 0.8kg / t of Suhong AA Plus high-temperature α-amylase, stir and mix evenly.
[0035] (2) Liquefaction: Continuous jet liquefaction at 108-110°C, heat preservation treatment of liquefied liquid at 95-98°C for 140 minutes.
[0036] (3) Saccharification: Cool down the temperature of the liquefied liquid to 54-58°C, pH value is 4.1-4.4, add 1.0kg / t Suhong GA II or DextrozymeGA glucose glucoamylase for saccharification for 50 hours, add 0.01kg / t Shearzyme500L pentosanase , Finizym W lysophospholipase for hydrolysis, and then hydrolysis for 15 hours, the DE value of the product was 98.2.
[0037] (4) Protein conversion: the material temperature is 56-50°C, the pH val...
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